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Molecular marker KB1 for identifying clubroot resistance of brassica rapa pekinensis, primer and application

An anti-clubroot, molecular marker technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. The results are easy to confuse and other problems, so as to achieve the effect of simple and fast detection, accurate distinction and good specificity.

Active Publication Date: 2018-05-11
TIANJIN RES INST OF VEGETABLE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some molecular markers linked to this site have been published, their linkage is not high enough, and there may be some false positives, or when testing, the difference between resistant and susceptible materials is not obvious, and the results are easy to confuse

Method used

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  • Molecular marker KB1 for identifying clubroot resistance of brassica rapa pekinensis, primer and application
  • Molecular marker KB1 for identifying clubroot resistance of brassica rapa pekinensis, primer and application
  • Molecular marker KB1 for identifying clubroot resistance of brassica rapa pekinensis, primer and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Chinese cabbage contains clubroot disease resistance gene Crr1a Obtaining of F2 segregating populations

[0032] The Chinese cabbage clubroot disease-resistant material G6 was used as the female parent, and the susceptible material G4 was used as the male parent to obtain the F1 generation. The F1 generation was tested for disease resistance in the field and all of them were disease-resistant materials. The segregation population of F2 generation obtained by selfing of F1 generation was used for phenotype identification and molecular marker detection of Chinese cabbage clubroot.

Embodiment 2

[0034] Phenotypic Identification and Molecular Marker Detection of Clubroot Resistance in F2 Generation Segregation Population of Chinese Cabbage

[0035] 1. Identification of clubroot resistance phenotypes in isolates

[0036] Taking the F2 population obtained in Example 1 as material, inoculate Physiological race No. 4 of Plasmodium phylloxera after sowing, the inoculation and investigation methods refer to the literature "Wang Weihong et al., Identification of Physiological Races of Cruciferous Vegetable Physiological Races and Resistance Source Screening, Chinese Vegetables, 2013 Vol. 1(12): 55-60". The results showed that among the 66 F2 generation Chinese cabbage plants, 46 were resistant and 20 were susceptible ( figure 1 )

[0037] Molecular Marker Detection of Clubroot Resistance in F2 Population of Chinese Cabbage

[0038]2. Use the CTAB method to extract the genomic DNA of the leaves of the clubroot-resistant material G6, the susceptible material G4, and the F2 ...

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Abstract

The invention discloses an InDelmolecular marker KB1 co-separated with a brassica rapa pekinensis clubroot resistance gene Crr1a, a primer and application of the molecular marker. The invention provides a method for identifying or auxiliary identifying whether brassica rapa pekinensis contains the clubroot resistance gene Crr1a or not. The method comprises the steps of adopting a brassica rapa pekinensis genome to be detected as a template; carrying out PCR (Polymerase Chain Reaction) amplification by the primer provided by the invention; according to the size of an amplified product, judgingwhether the brassica rapa pekinensis to be detected contains the clubroot resistance gene Crr1a or not and is the brassica rapa pekinensis with clubroot resistance or not. The molecular marker provided by the invention is utilized for auxiliary selecting the brassica rapa pekinensis with clubroot resistance, is high in accuracy, low in cost, and time and labor saving.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker KB1 for identifying clubroot resistance of Chinese cabbage, a primer and an application thereof. Background technique [0002] Chinese cabbage ( Brassica rapa L. ssp. pekinensis ) belongs to the Brassicaceae genus, and is one of the vegetable crops with the largest planting area and output in my country. Clubroot is caused by the clubroot fungus ( Plasmodiophora brassicae Woronin), which can cause abnormal swelling and nodules in the roots of cruciferous plants such as Chinese cabbage, is a serious worldwide root disease of cruciferous plants, which can cause global The 10%-15% reduction in production, in areas with severe disease, even leads to complete production, which seriously threatens the production of cruciferous vegetables. The disease was first discovered in Europe. In recent years, there have been reports on the occurrence of clubroot in most prov...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王超楠华德平黄志银李梅范伟强王涛
Owner TIANJIN RES INST OF VEGETABLE
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