Annual culturing method for marine medaka in lab
A breeding method and laboratory technology, which is applied in the field of laboratory annual breeding of seawater medaka (also known as black-spotted medaka), can solve the problems of limited breeding conditions, inability to continue spawning, difficult breeding conditions, etc., and reach the market Good prospects and scientific research significance, good for growth and reproduction, and the effect of simplifying the breeding process
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[0016] Specific embodiments of the present invention will be described in detail below.
[0017] A species of seawater medaka (Black-spotted medaka, Oryzias melastigma ) laboratory annual cultivation method, characterized in that: the cultivation conditions of the cultivation method are as follows:
[0018] Breeding water: the seawater after five-stage filtration of nylon membrane, biosorbent, activated carbon, biochemical filter and ultraviolet sterilization system, the salinity is controlled at 28-30, and the dissolved oxygen level is 4-8mg / L.
[0019] Breeding water temperature: 26-30 degrees Celsius,
[0020] Breeding density: the growth period is 0.2-0.5L / tail, and the black-spotted medaka that has grown for more than three months enters the breeding period, and the breeding density during the breeding period is 0.5-1L / tail.
[0021] Photoperiod: 14 hours of light, 10 hours of darkness, and the lighting time is 5:00-19:00
[0022] Breeding sex ratio: male: female = 2:3...
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