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Microalgae culturing method and grease producing method

A technology of microalgae and algal liquid, applied in the methods of using microorganisms, methods based on microorganisms, microorganisms, etc., can solve the problems of cumbersome process, degradation of chlorella quality, unfavorable clear liquid and other problems

Active Publication Date: 2014-12-24
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Heterotrophic culture requires the addition of organic carbon sources, such as glucose. Although heterotrophic culture can rapidly increase algae cell density and growth rate, the process must strictly control the contamination of bacteria, otherwise it will lead to culture failure, and the pellets of heterotrophic culture Algae quality will decrease
In addition, this method is obviously more cumbersome and costly
Chinese patents 200980128146.1, 201010545871.3 and 201110029154.x disclose similar microalgae culture methods, these methods include the heterotrophic culture step of microalgae species and the photoautotrophic culture step with algae cells obtained from heterotrophic culture as seeds, these methods It can improve the efficiency of microalgae cultivation, but the two separate steps obviously make the operation more complicated
This method still has the following deficiencies: ① adjust the strong pH pH to make the microalgae self-flocculate, which will increase the cost and affect the vitality of the microalgae, which is not conducive to the recycling of algae species; ② in some cases, a slight disturbance will make the microalgae Microalgae redispersion, which is not conducive to the separation of supernatant after sedimentation

Method used

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  • Microalgae culturing method and grease producing method
  • Microalgae culturing method and grease producing method
  • Microalgae culturing method and grease producing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The culture of microalgae is carried out in a 5L glass triangular flask, filled with 3-4L medium (the medium is prepared by the above method, and the culture water is filtered and sterilized tap water), and the OD value of the final algae liquid is 0.3-0.6. Insert the pellets Algae species, and add glucose at 20g / L, urea at 3g / L, and ethanol at 3ml / L. Use natural light as the light source, control the light intensity in the range of 2000-20000 lux, and cultivate at room temperature. Introduce 2% (v / v) CO 2 / air mixed gas for aeration culture, and the mixed gas is filtered and purified by filter membrane. Microalgae growth curve see figure 1 .

Embodiment 2

[0044] The culture of microalgae is carried out in a 5L glass triangular flask, filled with 3-4L medium (the medium is prepared by the above method, and the culture water is filtered and sterilized tap water), and the OD value of the final algae liquid is 0.3-0.6. Insert the pellets Algae species, and add glucose at 10g / L, urea at 2g / L, and ethanol at 5ml / L. Use natural light as the light source, control the light intensity in the range of 2000-20000 lux, and cultivate at room temperature. Introduce 2% (v / v) CO 2 / air mixed gas for aeration culture, and the mixed gas is filtered and purified by filter membrane. Microalgae growth curve see figure 1 .

Embodiment 3

[0046] The culture of microalgae is carried out in a 5L glass triangular flask, filled with 3-4L medium (the medium is prepared by the above method, and the culture water is filtered and sterilized tap water), and the OD value of the final algae liquid is 0.3-0.6. Insert the pellets Algae species, and add glucose at 15g / L, urea at 1.5g / L, and ethanol at 6ml / L. Use natural light as the light source, control the light intensity in the range of 2000-20000 lux, and cultivate at room temperature. Introduce 2% (v / v) CO 2 / air mixed gas for aeration culture, and the mixed gas is filtered and purified by filter membrane. Microalgae growth curve see figure 1 .

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Abstract

The invention relates to a microalgae culturing method and a grease producing method. The culturing method includes: adding glucose and an alcohol into a culture medium of microalgae, and culturing under photoautotrophy conditions, wherein the alcohol is methanol and / or ethanol. The culturing method and the grease producing method can be used for large-scale low-cost efficient culturing of the microalgae, guarantee rapid growth of the microalgae and allow the microalgae to have a high grease content.

Description

technical field [0001] The invention relates to a method for cultivating microalgae and a method for producing oil. Background technique [0002] Microalgae are a class of low-level plants that grow in water with a wide variety and are extremely widely distributed. It is a cell factory driven by sunlight, and absorbs CO through efficient photosynthesis of microalgae cells. 2 , convert light energy into chemical energy of carbohydrates such as fat or starch, and release O 2 . The use of microalgae to produce bioenergy and chemicals can simultaneously achieve "replacing fossil energy and reducing CO 2 The three purposes of "emission, purification of waste gas and sewage". The advantages of "microalgae biotechnology" lie in the following aspects: ① Microalgae is the original plant with the highest photosynthetic efficiency. Compared with crops, the yield per unit area is dozens of times higher Microalgae is also the fastest-growing plant in nature, usually within 24 hours, t...

Claims

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Application Information

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IPC IPC(8): C12N1/32C12P7/64C12R1/89
Inventor 荣峻峰周旭华朱俊英黄绪耕
Owner CHINA PETROLEUM & CHEM CORP
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