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Method for cultivating chroococcus by domestic-wastewater-diluted kitchen waste anaerobic digestion solution

A technology for domestic sewage and kitchen waste, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of reducing the concentration of nitrogen and phosphorus, and the high cost of microalgae cultivation, so as to increase the growth rate, Effect of increasing oil content and biomass content and reducing cost

Inactive Publication Date: 2016-04-13
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the above-mentioned problems of high cost of sewage treatment and microalgae cultivation, the present invention provides a method for diluting digestive juice with domestic sewage and using it to cultivate microalgae to obtain biomass
This method can not only solve the problem of high cost of microalgae cultivation, but also treat two kinds of sewage at the same time, reduce the concentration of nitrogen and phosphorus in water and obtain valuable biomass at the same time

Method used

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  • Method for cultivating chroococcus by domestic-wastewater-diluted kitchen waste anaerobic digestion solution
  • Method for cultivating chroococcus by domestic-wastewater-diluted kitchen waste anaerobic digestion solution
  • Method for cultivating chroococcus by domestic-wastewater-diluted kitchen waste anaerobic digestion solution

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Experimental program
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Effect test

Embodiment 1

[0020] Example 1 Growth characteristics of Chroococcus SDEC-6 in different culture media

[0021] The method of the present invention for diluting digestive liquid with domestic sewage and culturing Chroococcus SDEC-6 while obtaining biomass

[0022] Including the following steps:

[0023] (1) Put the Chroococcus SDEC-6 algae solution stored in the laboratory in BG11 medium for expansion. The composition of BG11 medium is as follows: NaNO 3 1.5g / L, K 2 HPO 4 0.04g / L,MgSO 4 .7H 2 O0.075g / L,CaCl 2 .2H 2 O0.036g / L, Citricacid 0.006g / L, Ferricammoniumcitrate 0.006g / L, EDTANa 2 0.001g / L,Na 2 CO 3 0.02g / L, A 5 1ml / L.

[0024] (2) The absorbance at 680nm of the obtained liquid seed liquid after ten times dilution is 0.203.

[0025] (3) Inoculate the seed liquid in the diluted sewage, conduct constant temperature cultivation in an artificial climate chamber, and sample the biomass every day during the cultivation process and conduct water quality analysis.

[0026] (4) After the algae body descr...

Embodiment 2

[0036] Example 2 Oil accumulation characteristics of Chroococcus SDEC-6

[0037] Oil content analysis:

[0038] Weigh about 0.1g of dry algae flour into a 50mL centrifuge tube, add 10mL methanol / chloroform (v / v=1:2) solution, treat with an ultrasonic breaker for 10min, centrifuge at 4000r / min for 10min, and separate the layers The organic phase in the two phases was transferred to a 60 mL separatory funnel, and the entire extraction process was repeated twice. According to the volume of the extract in the separatory funnel, add 0.9% sodium chloride solution (the added volume is 1 / 5 of the extract), shake well for about 1 min, and let stand for 15 min to measure the volume of the lower extract and take 5 mL in In a 10mL glass test tube, blow dry with nitrogen, and place the glass tube in an oven at 60°C to a constant weight (about 30min). The oil content is calculated as follows:

[0039] L W = ( m 2 - m 0 ) X V 5 X m 1

[0040] In the...

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Abstract

The invention discloses a method for cultivating chroococcus by a domestic-wastewater-diluted kitchen waste anaerobic digestion solution and belongs to the technical field of microalgae biology. The method is characterized in that BG11, domestic wastewater, a digestive fluid and culture solutions diluted by different times are utilized for constant-temperature light culture of chroococcus SDEC-6 and chroococcus SDEC-6 is harvested in the last growth stage. Results show that the biomass, the growth rate, the lipid content and the like of chroococcus SDEC-6 in domestic wastewater and at different dilution times are higher than those of BG11, the biomass and the lipid content, obtained by diluting a culture medium by 100 times, are higher than those of domestic wastewater. In conclusion, a mixed culture solution obtained by the domestic-wastewater-diluted kitchen waste anaerobic digestion solution can serve as a high-quality substitutive medium, promote biomass yield and lower the culture cost and is worthy of popularization.

Description

Technical field [0001] The invention belongs to the field of microalgae biotechnology, and particularly relates to a method and application for culturing Chroococcus using domestic sewage to dilute the anaerobic digestion liquid of kitchen waste. Background technique [0002] In recent years, due to the increasing energy demand of mankind, the widespread use of fossil fuels has not only caused a global energy crisis but also caused environmental pollution worldwide. The crisis of water resources and energy has become a major challenge facing mankind in this century. Finding an environmentally friendly new energy source is an effective means to solve these challenges. As an important part of the ecosystem, microalgae have the advantages of fast growth, short cycle, no arable land, and can be used to prepare biodiesel. However, due to its relatively high cost of cultivation, large-scale cultivation is relatively difficult. [0003] Many researchers have begun to use sewage to culti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C02F3/32C12R1/89
CPCC02F3/322C12N1/12
Inventor 裴海燕成娟韩飞蒋丽群聂昌亮张立杰
Owner SHANDONG UNIV
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