Microalgae cultivation method and grease production method
A technology of microalgae and algae liquid, applied in the field of microalgae cultivation, can solve the problems of high energy consumption, cumbersome process, and unsuitability for large-scale industrial production
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Embodiment 1
[0047] The cultivation of Chlorella is carried out by adopting the two-stage expanded basic technological process. Primary inoculation culture: 1L glass triangular flask is filled with about 700ml of culture medium, the medium is BG11 medium (Table 1), the primary inoculation medium uses urea as nitrogen fertilizer, the concentration is 0.3g / L, and the light and temperature are controlled appropriately. Inject 2% (v / v) CO2 air mixture for aeration culture, and the mixture is purified by filter membrane. Detect the OD value of the algae liquid. When the OD value of the algae liquid reaches more than 5, the secondary expansion culture can be carried out. Secondary expanded culture adopts the method of the present invention: expanded culture is carried out in 5L glass Erlenmeyer flask, packs 3~4L culture medium (culture medium mother liquor is through autoclaving high temperature sterilization or boiling disinfection, culture water adopts the tap water of filter disinfection), ac...
Embodiment 2
[0049] The culture of chlorella, method is the same as example 1, and difference is that the nitrogenous fertilizer that adds is urea, and addition is 9mg / L.d. Harvest after continuous cultivation for 20 days, its growth curve is shown in figure 1 .
Embodiment 3
[0051] The cultivation of Scenedesmus, method is with example 1. Harvest after continuous cultivation for 20 days, its growth curve is shown in figure 2 .
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