Method for producing oil through immobilized culture of microalgae
An immobilized culture, microalgae technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of exchanging the illumination frequency of algal cells, difficult to induce oil, and cannot directly use microalgae to immobilize oil production. and other problems to achieve the effect of filling the gap
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specific Embodiment 1
[0075] Evenly smear Scenedesmus species on the immobilized carrier, use full nutrient medium (BG11 liquid medium) to supplement water and nutrition, and pass through the culture medium containing 1% CO 2 of compressed air. During the experiment, the outdoor light intensity at noon was 1500μmol / m 2 / s, the light intensity in the morning and evening is about 300μmol / m 2 / s, the average light intensity during the day is 500μmol / m 2 / s, the average temperature is 25°C. Continuous culture for 7 days, at this time the cell concentration reached 110g / m 2 During the detection period, the chlorophyll fluorescence value (Fv / Fm) of each layer of cells on the growth carrier was stable at about 0.6 on average. Then change the concentration of sodium nitrate in the BG11 medium to 0.5g / L, and induce oil production for 5 days. During the process of inducing oil production, continue sampling to detect the chlorophyll fluorescence value of the microalgae cells on the growth carrier, wherein...
specific Embodiment 2
[0076] Evenly smear Scenedesmus species on the immobilized growth carrier, use full nutrient medium (BG11 liquid medium) to supplement water and nutrients, and pass through the culture medium containing 2% CO 2 of compressed air. During the experiment, the outdoor light intensity at noon was 1500μmol / m 2 / s, the light intensity in the morning and evening is about 300μmol / m 2 / s, the average light intensity during the day is 500μmol / m 2 / s, the average temperature is 25°C. Continuous culture for 4 days, at this time the cell concentration reached 60g / m 2, during which the photosynthetic oxygen evolution of the growth carrier algae cells was detected. Then change the concentration of sodium nitrate in the BG11 medium to 0.3g / L, and induce oil production for 5 days. Algae cell layer cells (the cell layer with almost no oxygen evolution and close to the carrier is classified as the regrowth microalgae cell layer, and the layer that detects a large amount of oxygen and is far ...
specific Embodiment 3
[0077] Evenly smear the chlorella species on the immobilized growth carrier, use the full nutrient medium (BG11 liquid medium) to supplement water and nutrients, and pass through the culture medium containing 2.5% CO 2 of compressed air. During the experiment, the outdoor light intensity at noon was 1500μmol / m 2 / s, the light intensity in the morning and evening is about 300μmol / m 2 / s, the average light intensity during the day is 500μmol / m 2 / s, the average temperature is 25°C. Continuous culture for 4 days, at this time the cell concentration reached 66g / m 2 During the detection period, the chlorophyll fluorescence value (Fv / Fm) of each layer of cells on the growth carrier was stable at about 0.6 on average. Then change the concentration of sodium nitrate in the BG11 medium to 0.4g / L, induce oil production and culture for 4 days, during the process of inducing oil production culture, take samples to detect the chlorophyll fluorescence value on the growth carrier, wherei...
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