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Application of lactobacillus plantarum DP189 in preparation of medicine for preventing and/or treating Alzheimer's disease

A technology of Alzheimer's disease and Lactobacillus plantarum, applied in the biological field, can solve the problems of delaying and preventing the occurrence and development of AD, and achieve the effects of shortening the escape latency period, improving the level, and improving the antioxidant capacity

Active Publication Date: 2021-06-08
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these drugs temporarily relieve AD symptoms by increasing neurotransmitter levels, but cannot delay or prevent the occurrence and development of AD

Method used

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  • Application of lactobacillus plantarum DP189 in preparation of medicine for preventing and/or treating Alzheimer's disease
  • Application of lactobacillus plantarum DP189 in preparation of medicine for preventing and/or treating Alzheimer's disease
  • Application of lactobacillus plantarum DP189 in preparation of medicine for preventing and/or treating Alzheimer's disease

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Experimental program
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Effect test

preparation example Construction

[0066] Preparation method of D-galactose solution: take D-galactose powder (Sigma, USA), and prepare D-galactose solution with a concentration of 12 mg / ml with physiological saline.

[0067] The preparation method of the aluminum chloride solution: take the aluminum chloride powder and prepare an aluminum chloride solution with a concentration of 2 mg / ml with physiological saline.

[0068] The content of total superoxide dismutase (SOD) in serum was detected with Nanjing Jiancheng total superoxide dismutase (SOD) assay kit (A001-3-2). Reduced glutathione (GSH) content in serum was detected using Nanjing Jiancheng Reduced Glutathione (GSH) Assay Kit (A006-2-1). The content of glutathione peroxidase (GSH-PX) in serum was detected with Nanjing Jiancheng glutathione peroxidase (GSH-PX) assay kit (A005-1-2). The content of malondialdehyde (MDA) in serum was detected by enzyme-labeled biological mouse malondialdehyde enzyme-linked immunosorbent assay kit (product number MB-5829A). ...

Embodiment 1

[0069] The preparation of embodiment 1 plant lactobacillus (Lactobacillus plantarum) DP189 bacterial suspension

[0070] 1. Strain activation: Pick the freeze-dried bacterial powder of Lactobacillus plantarum (Lactobacillus plantarum) DP189, inoculate it in MRS liquid medium, and cultivate the activated strain in a constant temperature incubator at 37°C for 16 hours.

[0071] 2. Strain subculture: take the activated culture medium of the strain, inoculate it into a new MRS liquid medium with an inoculation amount of 3%, and cultivate it in a constant temperature incubator at 37°C for 16 hours. Repeat this step to subculture the strain 3 times, repeat this step Carry out expanded culture to obtain more bacterial fluid.

[0072] 3. Calculation of the number of viable bacteria: Dilute the bacterial solution by 10 and 10 respectively by coating plate counting method 2 、10 3 、10 4 、10 5 、10 6 、10 7 、10 8 and 10 9 times, the diluted bacterial solution was evenly spread on th...

Embodiment 2

[0074] Example 2 Effects of Lactobacillus plantarum (Lactobacillus plantarum) DP189 on preventing and treating Alzheimer's disease

[0075] 1. Materials and methods:

[0076] 1. Establishment of Alzheimer's disease mouse model and experimental grouping

[0077] 30 SPF-grade ICR mice, half male and half female, weighing 20±5 (g), kept at constant temperature (20±2°C) and constant humidity (40-60%), kept circadian rhythm for 12 hours, free to eat and drink, and fed normally for one week , were randomly divided into 3 groups according to body weight and sex: blank control group (n=10), AD model group (n=10), DP189 prevention group (n=10), AD model group was fed with sterile normal saline every day, 90min Afterwards, intraperitoneal injection of D-galactose solution (120mg / kg), intragastric administration of AlCl 3 solution (20mg / kg); the DP189 prevention group was intragastrically administered Lactobacillus plantarum DP189 bacterial suspension every day, and after 90 minutes, i...

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Abstract

The invention relates to application of lactobacillus plantarum DP189 in preparation of medicine for preventing and / or treating Alzheimer's disease, and belongs to the field of biology, the lactobacillus plantarum DP189 is deposited in China Center for Type Culture Collection on March 25, 2019, and has a deposit number of CCTCC NO: M2019199. The lactobacillus plantarum DP189 is prepared into bacterial suspension with number of viable bacteria greater than or equal to 1 * 10 < 9 > CFU / ml. The lactobacillus plantarum DP189 can improve learning and memory ability, improve cognitive impairment, relieve damage degree of hippocampal neurons, reduce a level of malonaldehyde in serum, improve a level of superoxide dismutase in serum and improve levels of glutathione and glutathione peroxidase in serum; reduces levels of interleukin-1beta and tumor necrosis factor-alpha in serum, increases a level of interleukin-6 in serum, increases a level of dopamine in serum, and increases a level of 5-hydroxytryptamine in serum.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of plantarum lactobacillus DP189 in the preparation of drugs for preventing and / or treating Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD) is a degenerative disease of the central nervous system and belongs to the common type of senile dementia. depression etc. According to the international AD report, more than 50 million people worldwide suffer from AD or other types of dementia in 2019. It is estimated that the number of patients will reach 152 million in 2050, and a patient will be diagnosed every three seconds. The main pathological features of AD include deposition of β-amyloid protein (Aβ) to form neuritic plaques, hyperphosphorylation of Tau protein leading to neurofibrillary tangles, loss of neurons and synapses, and activation of microglial cells. So far, a large number of studies on the disease have not been able t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P25/28C12N1/20C12R1/25
CPCA61K35/747A61P25/28C12N1/20
Inventor 李盛钰赵子健赵玉娟王超杨舸高磊
Owner JILIN ACAD OF AGRI SCI
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