125 results about "Water maze" patented technology

The invention discloses a closed rotary maze device for an animal memory training system. The maze device comprises a round box body and a circuit control system. Key technical points of the maze device are that: the bottom of the box body of the maze device is provided with a turntable driven by a motor; the motor is arranged on a central cylinder, and an output shaft of the motor is in fastenedwith the turntable along the axial direction of the central cylinder; the turntable rotates around the central cylinder under the driving of the motor; the periphery of the lower part of a frame of the box body is provided with a plurality of pulleys for supporting the turntable; and a sieve-shaped supporting net is arranged above the turntable. The maze device overcomes the defect of the lateralization in the detection of the conventional maze devices such as a Y-maze and a T-maze. In addition, a device driven by gradient voltage is removed; the maze device effectively prevents animals from finishing experiments by means of smell, and respective disadvantages of a Morris water maze, the Y-maze and a drought maze are weighted; the starlight detection can be performed through program control according to the nocturnal habits of the experimental animals; and the maze device can ensure that the experimental animals are subjected to sensory stimulation in real environment, which is more favorable for acquisition of experimental data.

The invention provides a method and a device for enhancing spatial memory of water maze test animal. The method comprises a water tank for animal swimming and a platform arranged in the water, a camera and a computer system. The invention is characterized in that the method also comprises a visual reference system; the visual reference system arranges a septum above the surrounding of the water tank to isolate interference from outside; the visual reference system also arranges a plurality of fixed pictures on the internal side of the septum as memory reference of the animal and the fixed picture is contrast with the color of the septum. By building the water maze standard visual reference system, the invention ensures that the test animal can avoid interference from outside in the process of swimming, meanwhile, when the test animal swims in the water tank, the fixed picture in the system as the visual reference of the animal is good for enhancing spatial memory ability of the animal and reducing system error of a plurality of repeated tests. The test result shows that the invention can significantly enhance spatial memory of a rat in the water maze test and ensures that the spatial memory can maintain a longer time.

The invention relates to a qingkailing preparation and the application of a composition of effective components thereof including baicalin, jasminoidin, cholic acid and mother of pearl to improving cognitive dysfunction, preventing and treating senile dementia. Morris water maze experiments prove that the qingkailing preparation and the effective components thereof can improve the cognitive dysfunction of senescence accelerated mice; an in-vivo experiments prove that the qingkailing preparation can improve plasticity change of Alzheimers disease model rats synapse damage resulting from A beta 1-42 hippocampal injection; an in vitro experiments prove that the qingkailing preparation and the composition of the effective components thereof including baicalin, jasminoidin, cholic acid and the mother of pearl can improve complete cerebral acetylcholine content of the senescence accelerated mice, can cause relative increment in the dopamine and serotonin levels of central nerve by staying the degradation of the acetylcholine, the dopamine and the serotonin, thus improving the intracerebral monoaminergic system activity, therefore, the Morris water maze experiments are possibly related to the effect of the qingkailing formula on improving the cognitive dysfunction and preventing and treating the senile dementia.

The invention discloses a preparation method of a walnut active peptide for improving the memory function, and belongs to the technical field of active peptide preparation. Degreased walnut dregs usedas a raw material are crushed and homogenized, then are fully hydrolyzed by an alkaline protease and a neutral protease through a two-step controlled enzymatic hydrolysis technology, and undergoes amembrane separation technology to obtain the polypeptide with having a molecular weight of above 10 kDa, the polypeptide with having a molecular weight of 10~3 kDa and a polypeptide having a molecularweight of below 3 kDa. The method allows the walnut peptides with high hydrolysis degree and high activity to be obtained under the co-action of above two enzymes. The influences of the peptides on improvement of the memory of experimental animals are evaluated by using spatial exploration test, navigation test, step-through test and step-down test in a mouse Morris water maze as indicators. Results show that the small peptide (<3 kDa)with a short peptide chain can improve the spatial cognition and learning ability of dysmnesia mice, is a food-borne peptide, has a high safety, and can be taken for a long term.

The invention discloses a butyrylcholine esterase selective inhibitor and a preparation method and application thereof. The invention discloses a compound shown in a formula (I), and also discloses application of the compound in preparation of medicines for preventing or treating Alzheimer's disease. An inventor uses the inhibiting activity of butyrylcholine esterase, selective screening and Morris water maze experiment as carriers to evaluate the function of the compound in the formula (I) for treating the Alzheimer's disease (especially medium and severe Alzheimer's disease), the good in-vitro and in-vivo activities and higher selectivity are found, and the compound can be further developed into a precursor substance which can selectively inhibit the butyrylcholine esterase to reach thefunction of treating the Alzheimer's disease. The formula (I) is shown in the specification.

The invention discloses a traditional Chinese medicine composition for improving memory and a preparation method thereof. The composition is prepared from such medicinal raw materials as panaxadiol glucoside, ophiopogon root, asparagus, spina date seed, platycladi seed, dangshen, radix scrophulariae, dogwood, fructus alpiniae oxyphyllae, semen cuscutae and the like. The invention also provides a preparation method of the traditional Chinese medicine composition for improving memory. For a mouse dosed with the traditional Chinese medicine composition disclosed by the invention, in a step-down test and an Morris water maze test, the number of errors is decreased significantly (P is smaller than 0.05, P is smaller than 0.01), which is obviously superior to that of a control group and has an obvious difference with the control group (P is smaller than 0.05, P is smaller than 0.01), so that the traditional Chinese medicine composition disclosed by the invention can be considered to play a memory perfecting and consolidating effect on the mouse.

The invention belongs to the field of chemical pharmaceutical technology, and in particular relates to application of a genistein derivative 4', 5'-dyhydroxy-7-[4-(N, N-diethylamino) butoxy] isoflavone in preparing a medicament for treating learning and memory disorder. 4', 5'-dyhydroxy-7-[4-(N, N-diethylamino) butoxy] isoflavone has the effect of promoting macrophage chemotactic fator (MCF)-7 cell proliferation and the action effect is in a bell jar type. The latent time of female castrated mice (oophorectomy is performed) in a water maze experiment is shortened and the error time of the mice is greatly decreased in a Y maze experiment. The fact indicates that 4', 5'-dyhydroxy-7-[4-(N, N-diethylamino) butoxy] isoflavone can effectively improve learning and memory capability. The improvement of learning and memory is consistent with the enhancement of phosphorylation of calcium-calmodulin dependent protein kinase (CaMk) II. 4', 5'-dyhydroxy-7-[4-(N, N-diethylamino) butoxy] isoflavone has good effects of improving learning and memory and can be used for preparing the medicament for improving learning and memory.

The invention relates to a building method and application of an STCH (stress and chaperone) gene knock-out animal model. The model can function as an animal model for the research of occurrence of depression and / or dementia. The model is prepared by using a gene knock-out technique, animal behavior detections show that a mouse suffers depression-like symptoms (forced swimming test and tail suspension test) and memory dysfunction (water maze test) while it grows older and has depression and dementia-like symptoms, and the model is applicable to screening anti-depression and anti-dementia drugs.

The invention provides a high-grade full-automatic intelligent analysis system of a Morris water maze. The system comprises a water basin and a bracket for arranging the water basin and is characterized in that the water basin is provided with a water draining opening; the system further comprises a light uniform distribution system which is installed in the water basin, wherein the light uniform distribution system comprises a plurality of spot lights which have the same light and shade degree; light spots which are uniformly distributed on the periphery of the water basin; and the system further comprises a mobile terminal and an adapted connector of a desk type terminal and the connector is used for transmitting experimental data and control signals among a control cabinet, a plurality of platforms and a plurality of terminals. The invention further provides a corresponding expansion system. The high-grade full-automatic intelligent analysis system of the Morris water maze has the advantages that: the interference to experiments by the outer side is reduced, the scene distinguishing is easy to realize, the water drainage is controlled by an electronic valve, a water faucet is prevented from being forgotten closing by a water level limited valve and the like; therefore, the analysis system can be applied to biological experiments of scientific research academies.

The invention discloses an annular platform used for testing the learning and memory capacity of a mouse and a control method thereof. The annular platform comprises a support (1), a working platform (2), a camera (5), a sound box (6), an information acquisition controller (7), a computer (8), etc. The computer (8) outputs a control signal according to a designed test plan and displays test data and images on an operational interface. The technology and method having good versatility and expandability can not only be applied to the programmed control and test of the annular platform, but also be applied to other ethological apparatuses such as a water labyrinth, an autonomous activity apparatus, an exploration exercise tester, etc. after undergoing corresponding transformations.

The invention discloses a composition of extractives of hedysarum polysaccharide and rhodiola rosea, a preparation method thereof and application of compatibility of the same in preparing medicines for treating AD. The compatibility of the extractives of hedysarum polysaccharide and rhodiola rosea in the invention can remarkably protect SH-SY5Y cells from being damaged by Abeta25-35 and increase the viability of the SH-SY5Y cells; according to results of Morris water maze tests, it is revealed that the extractives of hedysarum polysaccharide and rhodiola rosea can substantially enhance learning and memory ability and cognitive impairment of AD model rats; according to results of further detection, the compatibility of the extractives of hedysarum polysaccharide and rhodiola rosea enables the level of glucose metabolism in brains of the AD model rats to recover, which indicates that the compatibility of the extractives of hedysarum polysaccharide and rhodiola rosea has the effect of treating AD and has a remarkable effect.

The invention discloses an animal learning memory individualized evaluating method based on a water maze. The animal individualized evaluating method is used for determining cognition abnormity by analyzing data of the time (incubation period) from water entering to platform boarding of multiple batches of mice / rats with a Morris water maze experiment as a tool and normal animals and cognition abnormal animals as the objects. Compared with a traditional method emphasizing comparison among groups of samples, the method has the advantages that the overgeneralization means of the pure 'average value + / - standard deviation' form in the traditional method is abandoned, and model animals are separated according to the cognition difference and studied one by one; by means of the method, the animal cognition level difference can be more objectively reflected, and the state is closer to the real state of animals; the method is easy to operate and feasible, and the reliability and scientificity of the method are ensured through repeated experiments of multiple batches of animals and the confirmatory experiments of models.

The invention discloses application of cobra neurotoxin monomer molecules in treatment of senile dementia. The senile dementia, also known as Alzheimer disease, is a senile disease with progressive memory disorder, obstacle of judgment and reasoning ability as well as dyskinesia as main clinical features. Evidence shows that strong focal inflammatory responses exist in the brains of the patients with the senile dementia, and activated microglia and astrocytes exist near senile plaques; and the activated microglia and astrocytes can express a plurality of inflammatory cytokines, including interleukin-1[beta] (IL-1[beta]), tumor necrosisfactor-alpha (TNF-alpha) and the like. Therefore, experts suggest that neurodegeneration may be caused by inappropriate activation of immune and inflammatoryreactions in the brain; and super-strong immune reactions can attack nerve tissue "in the wrong direction", thereby causing neuron damage and death. In order to solve the problems, the invention discloses a group of cobra neurotoxin monomer molecules which can inhibit increase of contents of related inflammatory factors, namely the IL-1[beta]) and the TNF-alpha, in the hippocampus of rats with the senile dementia, as well as significantly shorten escape latency of the rats with the senile dementia after treatment in Morris water maze experiment.

The invention discloses application of a composition and a compatible method of ligustrazine, tenuigenin and stibene glucoside in preparation of a drug for treating Alzheimer disease. In the invention, the composition of ligustrazine, tenuigenin and stibene glucoside can be compatible according to a ratio to obviously prevent damage on the cognitive function of APP695V717I transgenic mice; proved by a Y electric maze experiment and a Morris water maze experiment, the composition of ligustrazine, tenuigenin and stibene glucoside can obviously improve the capabilities of passive avoidance, active avoidance and spatial memory exploration competence of the model mice, and has the obvious functions of preventing ageing hypomnesia and dysmnesia related to the Alzheimer disease; the further detection shows that the compatible composition of ligustrazine, tenuigenin and stibene glucoside can reduce deposition of age pigments in brains of AD transgenic mice, and improve the activity of acetylcholine esterase, so as to indicate that the composition of ligustrazine, tenuigenin and stibene glucoside compatible according to the ratio can treat the Alzheimer disease remarkably.

The invention relates to a fishing, temporarily-storing and parching device special for water maze experimental rats. The device comprises a moving frame body, experimental rat storing cages and an experimental rat temporarily-storing platform, the moving frame body has four layers, multiple experimental rat cage placement cells are made on each layer, each experimental rat storing cage is slidably mounted in the corresponding experimental rat cage placement cell, an infrared electric parching lamp is arranged at the top end of each experimental rat cage placement cell, a retaining plate is hinged at the top end edge of each experimental rat cage placement cell, the lower end of each retaining plate does not contact with the corresponding experimental rat storing cage, and an experimentaltool storing cabinet and the experimental rat temporarily-storing platform are arranged on the lower portion of the moving frame body; a water maze special lamp mechanism is arranged at the top end ofthe moving frame body and comprises an inverted lamp cover and an incandescent lamp mounted in the lamp cover, and one side of the inverted lamp cover is connected with the top end of the moving frame body through hinges. The device is simple in structure, simple and convenient in operation and capable of greatly improving water maze experiment efficiency.

The invention discloses a moringa seed and novel use of a moringa seed extract for medicine, health-care food or food capable of promoting learning memory. Proved by a pharmacological experiment, when hyoscine is used for inducing learning amnesia hyoscine, the moringa seed extract is capable of improving an incubation period and reducing the amount of errors remarkably in an avoidance test, and improving an incubation period, increasing the amount of platform passing times and prolonging target quadrant stopping time of a mice remarkably in a water maze test; the moringa seed extract has an exact function of promoting learning memory and can be applied to improvement on learning memory amnesia caused by various reasons.

The invention relates to a construction method of an AD model rat hippocampus difference gene expression chart by combining Abeta and D-gal. The construction method comprises the following steps: firstly, treating a rat with the Abeta and the D-gal to establish an AD model rat; secondly, measuring memory damage of the AD model rat by using a Morris water maze so as to determine successful construction of the AD model rat; thirdly, obtaining hippocampus samples of an AD model group and a normal control group, and respectively obtaining gene chip data of all tested hippocampus gene expressions;fourthly, uploading all the gene chip data to gene chip data analysis software, obtaining differential expression genes and screening a gene spectrum of the differential expressions of the AD model rat; fifthly, carrying out functional annotation classification and a biological pathway analysis on the differently-expressed genes to reveal molecular basis associated with the AD. According to the construction method disclosed by the invention, the Abeta and the D-gal are used as a whole AD model to study the gene expression spectrum of the AD model rat hippocampus genes, so that other errors changing the expressions and induced by exogenous genes are effectively avoided.

The invention provides new application of green tea polyphenol in preventing and treating psychological stress injuries, which further shows that the green tea polyphenol has application in preparing medicaments or foods for preventing and treating symptoms or diseases correlative with the psychological stress injuries. The green tea polyphenol can improve the prolongation of latency and the reduction of grill-passing times and erection times of a Wistar rat in an open field test in a psychological stress state; the latency is prolonged and the number of electric shocks is increased in a step-through test; and the performance that the time for finishing a water maze and the number of errors are obviously increased is abnormal, which shows that the green tea polyphenol can improve the space exploration capacity, the cognitive function and the learning and memory ability of a psychological stress animal, has the effect of preventing and treating the psychological stress injuries, and is hopeful to be used for preparing the medicaments or the foods for preventing and treating the symptoms or the diseases correlative with the psychological stress injuries.

The invention discloses an application of cornflower-3-O-galactoside in preparing foods or medicines for improving cognitive functions of old people. Results from animal experiments show that: compared with a control group (mice are fed with distilled water), the step-through latencies of animals fed with the cornflower-3-O-galactoside are all evidently prolonged; the escape latencies of mice in Morris water maze are evidently shortened; the duration time on a platform quadrant is evidently prolonged, and times of crossing the platform are evidently increased. The hint shows that the spatial memory ability of the old-aged mice compensated with the cornflower-3-O-galactoside can be evidently improved. Compared with an H2O2 model group, the LDH activity of hippocampal nerve cell supernate inthe group fed with the cornflower-3-O-galactoside evidently decreases and the nerve cells apoptosis rate evidently decreases. The hint shows that the cornflower-3-O-galactoside can evidently suppressthe hippocampal nerve cell apoptosis induced by H2O2 and play a protection role in the hippocampal nerve cells damaged by H2O2.

The invention relates to application of lithium chloride to preparation of a methamphetamine cognitive disorder medicament. The experiments of the invention prove that a treatment effect of the lithium chloride on the methamphetamine cognitive disorder is reflected to show that the lithium chloride can serve as a new medicament for improving methamphetamine cognitive disorder by observing that methamphetamine has an inhibit effect on learning and memory of a rat, while the lithium chloride has an obvious promoting effect on the learning and memory of a mouse which is addicted to amphetamines in a water maze test of the lithium chloride on the mouse which is addicted to methamphetamines.

The invention discloses a spica prunellae extract, and a preparation method and application thereof. The weight percentage of caffeic acid in the spica prunellae extract is greater than or equal to 5 percent; the weight percent of rosmarinic acid is greater than or equal to 15 percent. The preparation method of the spica prunellae extract provided by the invention is simple, convenient and practical; the operation is easy; the spica prunellae extract is more suitable for industrial production; in addition, the extract has an improving effect on the study and memory of fast-aging model rats due to D-galactose; the escape latency period and the platform passing times of the fast-aging model rats in the water maze tests can be reduced; immunohistochemical results show that the extract can reduce the hippocampal CA1 area Caspase-3 and cerebral cortex GFAP expression of the fast-aging model rats. HE and Nissl dyeing results show that the extract can relieve the neuron injury due to the D-galactose. The condition that the spica prunellae extract has the effect of resisting Alzheimer's diseases is discovered; the spica prunellae extract is applied to the preparation of medicines for resisting the Alzheimer's diseases and for treating senile dementia.

The invention discloses application of mental treasure pills in preparation of a medicine for treating senile dementia. Due to the improvement effect of the mental treasure pills on mouse reproduction memory disorder caused by scopolamine and mouse memory consolidating hinder caused by NaNO2 and mouse reproduction memory disorder caused by ethanol through a step-down test and a Morris water maze test, the mental treasure pills have a certain effect of improving the learning and memory. In addition, the Morris water maze test of APP / PSI double transgenic mice further proves that the mental treasure pills have a certain effect of improving the dysmnesia. The experimental result proves that the mental treasure pills can be used for preparing the medicine for treating senile dementia and have the characteristics of stable curative effect, medication safety, low toxic-side effect, low price and the like.

Disclosed is a composition for treating or preventing amyloid-related diseases including 4-O-methylhonokiol as an active ingredient. More specifically, a pharmaceutical composition including 4-O-methylhonokiol, which is effective for treating or preventing amyloid-related diseases such as Alzheimer's disease, cognitive disorder, defective memory, amyloidosis, etc. is disclosed. The inventors of the present disclosure have found out for the first time that 4-O-methylhonokiol inhibits the production of β-amyloid. It has been confirmed to be useful in treating or preventing amyloid-related diseases. Through animal tests including water maze test and passive avoidance test on mice, 4-O-methylhonokiol has been confirmed to be effective for amyloid-related diseases such as Alzheimer's disease, defective memory, cognitive disorder, and the like. It was further confirmed through acetylcholinesterase activity inhibition test using mouse brain cortex and hippocampus tissue that they are particularly effective in treating or preventing Alzheimer's disease among the amyloid-related diseases.

The invention discloses a novel application of red stilbene and red stilbene polysaccharide in preparing medicament for treatment of Alzheimer disease. Red stibene and red stilbene polysaccharide in the invention can protect SH-SY5Y cells from the damage from A beta 25-35, and increase the viability of SH-SY5Y cells. The Morris water maze test indicates that red stibene and red stilbene polysaccharide can improve the cognitive dysfunction of mice with rapid brain aging and rats injected with A beta and AD in the hippocampus. Further histology detection indicates that red stilbene and red stilbene polysaccharide can increase the contents of center neurotransmitters 5-HT, 5-HIAA, NE and DA in rapid aging mice brain, wherein the neurotransmitters are monoamines.

The present invention relates to experiment model for animal's learning memory and spatial cognitive action. The water maze image processing system includes one Morris maze barrel with definite four quadrant orientation and containing certain amount of water; one transparent stage for rat to search and land inside the barrel; and one down drainage hole in any point in the bottom of the barrel; aswell as characterized video camera, image monitor, image collecting card and computer connected together. The experiment method is that by means of the image monitor and the image collecting card, the video camera records automatically the tested animal's activity and relevant data, and the data are transmitted to the computer for real-time research and analysis.

The invention provides a gynostemma pentaphyllum active ingredient dammarane type triterpenoid saponin compound and a separation method thereof. Experiments show that the 8 separated dammarane type triterpenoid saponin compounds can obviously prolong the time of resisting normal-pressure closed hypoxia and sodium nitrite hypoxia of mice and reduce the death rate of acute hypoxia, and have a better anti-hypoxia effect; wherein the falling time of a mouse on the rotating rod can be obviously delayed, the load-bearing swimming time of the mouse is prolonged, and a good anti-fatigue effect is achieved; since the hypoxia can cause cognitive impairment of mice, and water maze experiments find that pretreatment of the active site of the gynostemma pentaphylla can protect the learning cognitive ability of the mice to a certain extent, the active site of the gynostemma pentaphylla can be used for preparing drugs for resisting fatigue, resisting oxygen deficiency and improving learning cognitive.

The invention discloses an application of aspirin in treatment of cognitive and behavioral disorders after epilepsy. The inventor creates a lithium chloride-pilocarpine induced rat model of chronic epilepsy, detects the spatial learning and memory of the rat through a Morris water maze, detects the behavior depression of the rat through a tail suspension test, and finds that the cognitive function and behavior disorder of the epileptic rat are obviously perfected when being dosed with the aspirin at the chronic epilepsy attack stage.

The invention relates to the technical field of a health-care food new preparation, in particular to walnut peptide colon positioning mini-pills and a preparation method thereof. Each of the walnut peptide colon positioning mini-pills provided by the invention consists of a pill core and a coating layer, wherein the pill cores are prepared from walnut peptide, an excipient, an adhesive and a wetting agent. For the prepared walnut peptide colon positioning mini-pills, the particle diameters are 10-2000[mu]m, the release degree in stimulated gastric juice within 120min is smaller than 2%, the release degree in stimulated intestinal fluid within 5h is smaller than 10%, and the release degree in stimulated colon fluid within 1h is greater than 95%. Compared with walnut peptide raw materials, the absorption efficiency in colons is obviously improved, and the health-care food new preparation has high oral administration biological availability. The walnut peptide colon positioning mini-pillsprovided by the invention can improve the spatial memory capacity of rats for the position of a platform, and increase the speed of the rats passing through a water maze.

The invention relates to the technical field of stem cells, in particular to a retinal progenitor cell and a preparation thereof having a function of treating degenerative retinal diseases. The preservation number of the retinal progenitor cell provided by the invention is CGMCC NO.10419; and the retinal progenitor cell, which can keep a good proliferation ability and stem cell properties, can take a good curative effect on treating the degenerative retinal diseases. Experiments show that the retinal progenitor cell that the preservation number is CGMCC NO.10419, as being injected into the vitreous cavities of RCS rats, can take a protective effect and achieve multi-focal visual electric-physiology display on retinal outer nuclear layer cells; and in dark adaptation, potential change conducted by retinal nerve photoreceptor cells in a retinal progenitor cell injection group is more obvious than that in a group which is just injected with an HBSS (Hanks balanced salt solution) buffer solution. A water maze test also indicates that the visual function of the rats in the retinal progenitor cell injection group is improved. In addition, all rats injected with the retinal progenitor cell that the preservation number is CGMCC NO.10419 are free from abnormity.