Walnut active peptide for improving memory function, and preparation method thereof

Abstract

The invention discloses a preparation method of a walnut active peptide for improving the memory function, and belongs to the technical field of active peptide preparation. Degreased walnut dregs usedas a raw material are crushed and homogenized, then are fully hydrolyzed by an alkaline protease and a neutral protease through a two-step controlled enzymatic hydrolysis technology, and undergoes amembrane separation technology to obtain the polypeptide with having a molecular weight of above 10 kDa, the polypeptide with having a molecular weight of 10~3 kDa and a polypeptide having a molecularweight of below 3 kDa. The method allows the walnut peptides with high hydrolysis degree and high activity to be obtained under the co-action of above two enzymes. The influences of the peptides on improvement of the memory of experimental animals are evaluated by using spatial exploration test, navigation test, step-through test and step-down test in a mouse Morris water maze as indicators. Results show that the small peptide (<3 kDa)with a short peptide chain can improve the spatial cognition and learning ability of dysmnesia mice, is a food-borne peptide, has a high safety, and can be taken for a long term.

Description

technical field [0001] The invention belongs to the technical field of active peptide preparation, and in particular relates to a walnut active peptide prepared from degreased walnut dregs with obvious memory function and a preparation method thereof. Background technique [0002] With the increase of global population aging, the incidence of a series of neurodegenerative diseases, such as Parkinson's disease (PD) and Alzheimer's disease (AD), which are closely related to memory impairment, is increasing year by year. It has brought a heavy burden to the family and society. At present, the main treatment drugs for patients with memory loss are chemically synthesized drugs such as donepezil hydrochloride and piracetam. Although they can improve the spatial learning and memory cognition ability of patients, they will also produce some adverse reactions, such as hallucinations, easily Mental disturbances of irritability and aggressive behavior. Therefore it is particularly im...

AI Technical Summary

Problems solved by technology

At present, the main treatment drugs for patients with memory loss are chemically synthesized drugs such as donepezil hydrochloride and piracetam. Although they can improve the spatial learning and memory cognition ability of patients, they will also produce some adverse reactions, such as hallucinations, easily Mental disorder with irritability and aggressive behavior

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