702 results about "Hippocampus" patented technology

A system and / or method treating for a neurological disorder by brain region stimulation. The system and / or method comprises a probe and a device to provide stimulation. The probe has a stimulation portion implanted in communication with a predetermined brain region site. The stimulation portion of the probe may be implanted in contact with a predetermined brain region.

This technology relates generally to compounds and methods for stimulating neurogenesis (e.g., post-natal neurogenesis, including post-natal hippocampal and hypothalamic neurogenesis) and / or protecting neuronal cell from cell death. Various compounds are disclosed herein. In vivo activity tests suggest that these compounds may have therapeutic benefits in neuropsychiatric and / or neurodegenerative diseases such as schizophrenia, major depression, bipolar disorder, normal aging, epilepsy, traumatic brain injury, post-traumatic stress disorder, Parkinson's disease, Alzheimer's disease, Down syndrome, spinocerebellar ataxia, amyotrophic lateral sclerosis, Huntington's disease, stroke, radiation therapy, chronic stress, abuse of a neuro-active drug, retinal degeneration, spinal cord injury, peripheral nerve injury, physiological weight loss associated with various conditions, as well as cognitive decline associated with normal aging, chemotherapy, and the like.

Transcriptomes of individual neurons provide rich information about cell types and dynamic states. However, it is difficult to capture rare dynamic processes, such as adult neurogenesis, because isolation from dense adult tissue is challenging, and markers for each phase are limited. Here, Applicants developed Nuc-seq, Div-Seq, and Dronc-Seq. Div-seq combines Nuc-Seq, a scalable single nucleus RNA-Seq method, with EdU-mediated labeling of proliferating cells. Nuc-Seq can sensitively identify closely related cell types within the adult hippocampus. Div-Seq can track transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Dronc-Seq uses a microfluidic device to co-encapsulate individual nuclei in reverse emulsion aqueous droplets in an oil medium together with one uniquely barcoded mRNA-capture bead. Finally, Applicants found rare adult newborn GABAergic neurons in the spinal cord, a non-canonical neurogenic region. Taken together, Nuc-Seq, Div-Seq and Dronc-Seq allow for unbiased analysis of any complex tissue.

The invention provides a robot navigation map construction method based on rat brain hippocampus spatial cells. According to an information transfer loop of spatial navigation related cells in a hippocampus structure of a mammal, a robot acquires a current autonomic movement clue and color depth image information through environment exploration. The autonomic movement clue progressively forms coding to the spatial environment through path integration and characteristic extraction of the spatial cells in the hippocampus structure. The place field of place cells is gradually formed in an exploration process and furthermore covers the whole spatial environment, thereby forming a cognitive map. Meanwhile, Kinect acquires color depth image information of a view scene right in front of the current position as an absolute reference for performing closed-loop detection on the path and correcting a path integration error. At a closed-loop point, a system resets spatial cell discharging activity and performs correction on the path integration error. Nodes on a final navigation map comprise place cell mass coding information, corresponding visual cues and place topological relationships.

A clinically valuable method is provided for evaluating the onset or progression of Alzheimer's disease using a non-invasive biomarker obtained from an independent component analysis (ICA) of an individual's resting state functional MRI. The method is relatively more automated and objective than previous methods and exploits dysfunctional connectivity across an entire network of brain regions in Alzheimer's disease. It eliminates the need for investigator's intervention as much as possible and is more robust than structural and functional methods targeting the hippocampus.

The present invention relates to devices, systems, and methods for improving memory and / or cognitive function by brain delivery of compositions of small interfering RNA or vectors containing the DNA encoding for small interfering RNA. Such compositions can be administered using devices, systems and methods for direct delivery of the compositions to the brain, or using devices, systems, methods of delivery, and compositions that deliver small interfering RNA or vectors containing the DNA encoding the small interfering RNA across the blood-brain barrier. The present invention also provides valuable small interfering RNA vectors, and methods for reduction of BACE1 levels in the hippocampus, cerebral cortex, or other regions of the brain that have beneficial effects on improving memory and / or cognitive function in a subject.

A method and apparatus for treating Alzheimer's disease is disclosed. The method comprises delivering indomethacin or nonsteroidal anti-inflammatory agents having cyclooxygenase inhibitor action directly to the hippocampus or the lateral ventricle through an implanted catheter. The catheter has a flexible distal end that is implanted directly in the hippocampus or lateral ventricle as the preferred embodiment. The distal end has either a porous tip or a closed end. Where the distal end is closed, or a plurality of elution holes are present indomethacin is delivered to the hippocampus or lateral ventricle through either the porous tip or the elution holes. The catheter is part of a system for delivering indomethacin or nonsteroidal anti-inflammatory agents having cyclooxygenase inhibitor action to the hippocampus or lateral ventricle that includes a pump coupled to the catheter for delivering the indomethacin or nonsteroidal anti-inflammatory agents having cyclooxygenase inhibitor action through the catheter to the hippocampus or lateral ventricle.

The invention discloses a traditional Chinese medicine preparation for treating protrusion and hyperplasia of human cervical and lumbar vertebra, and belongs to the field of traditional Chinese medicines. The preparation is prepared from the following bulk drugs according to a gram weight ratio: 6 g of radix aconiti kusnezoffii preparata, 6 g of radix aconiti preparata, 15 g of lumbricus, 12 g of madder, 15 g of cassia seeds, 15 g of herba siegesbeckiae, 10 g of ophiopogon japonicas, 50 g of radix rehmanniae preparata, 15 g of alpinia oxyphylla, 15 g of gentiana macrophylla, 10 g of licorice, 15 g of alisma orientalis, 15 g of haw, 15 g of villous amomum fruit, 15 g of ligusticum wallichii, 15 g of hippocampus, 10 g of centipede, 10 g of scorpion, 20 g of gekko, 30 g of pseudo-ginseng, 20 g of phellodendron, 15 g of psoralea corylifolia, 30 g of speranskia tuberculata, 10 g of pyritum, 20 g of mollissima, 10 g of muscardine silkworm, 15 g of tortoiseshell, 30 g of ardisia gigantifolia, and the like. The bulk drugs are weighed for decoction, or weighed and immersed in wine, or weighed and mixed uniformly and grinded into powder for preparation of tablets or pills. The medicine has functions of relaxing sinews, activating collaterals, dispelling wind, eliminating dampness, activating blood, resolving stasis, dispersing nodules and repositioning. The effective rate reaches 98% and the curative rate reaches 95% or more.

Methods and compositions are provided for screening candidate compositions for activity with respect to treatment of aging-associated conditions, e.g., cognitive impairment conditions or age-related dementia. Aspects of the methods include administering a human blood product comprising plasma, to an immunocompromised animal, e.g., a mouse or a rat, and measuring the effect of the product on an endpoint, e.g., neurogenesis, locomotor activity, anxiety, spatial memory, and hippocampus-dependent memory.

The invention discloses a method for domesticating and breeding wild hippocampus. Mainly aiming at the problem of high death rate of wild hippocampus parents at present, the method is used for efficiently domesticating and managing the wild hippocampus, so that the death rate of the wild hippocampus parents can be remarkably reduced, and the mating success rate of the hippocampus and the survivalrate of the descendant young hippocampus can also be improved. The efficient and convenient method is provided for commercial culture of the hippocampus, and the method has important economic value.

The invention discloses cell frozen stock solution for freezing and storing nerve cells and a freezing storage method, wherein, the cell frozen stock solution and the freezing storage method are used for freezing and storing the nerve cells, especially primary animal nerve cells that are isolated freshly, such as hippocampus nerve cells. The frozen stock solution is formed by adding basic medium,fetal bovine serum, dimethyl sulfoxide,6-fructose phosphate and flavonoid compounds to the basic medium; 6-fructose phosphate (w / w) achieves the function of improving cell viability (effective cell rate) when the frozen cells are recovered; and in addition, the flavonoid compounds achieve definite oxidation resistance and can prevent free radical from damaging the cells. The cell frozen stock solution and the freezing storage method can improve the survival rate and the cell function of the nerve cells through freezing storage, the survival rate of unfreezing cells can be increased to reach more than 95 percent, the frozen stock solution for the nerve cells can preserve the nerve cells for a long period, the biological activity of the nerve cells can be ensured, and very high utility values can be achieved.

The invention discloses a hippocampus extraction method of a human brain nuclear magnetic resonance image based on a 3D neural network. The method comprises the following steps: preprocessing an original image data set and a label, constructing a 3D hippocampus segmentation neural network model, defining a boundary enhancement loss function, optimizing the boundary enhancement loss function, and detecting a preprocessed to-be-detected image by using the trained 3D hippocampus segmentation neural network model to obtain a hippocampus extraction result. According to the method, the 3D hippocampus segmentation neural network model is utilized, efficient, automatic and accurate segmentation of the hippocampus structure in human brain MRI is achieved, and the time for a doctor to perform earlydiagnosis on Alzheimer's disease can be shortened.

The invention relates to method(s) of use of the compound(s) described herein, e.g. method for stimulating neurogenesis, including in vitro neurogenesis, by contacting neuronal progenitor cells with an effective amount of the compound(s) described herein; method for treatment of a subject in need of treatment with a neurogenic compound; and / or for treatment of a disease or condition associated with damage to the hippocampus. The subject may be a human or a veterinary animal.

The invention discloses a healthcare wine for improving immunity. The healthcare wine is prepared from the following components by weight (gram): morinda officinalis, loranthus parasiticus, semen cuscutae, Chinese yam, radix ophiopogonis, angelica sinensis, fructus schizandrae, flos carthami, ligusticum wallichii, medicated leaven,ginger mangnolia officinalis, unhairing rosa laevigata michx, polygonum multiflorum, pulp of dogwood fruit, earthworm, clematis chinensis, pawpaw, herba epimedii, rhizoma cibotii, shawanzi (Chinese character), codonopsis pilosula, poria cocos, semen coicis, asparagus fern, radix scrophulariae, polygala tenuifolia, peach seed, panax notoginseng, rhizoma dioscoreae nipponicae, costusroot, jujube, mulberry, radix glycyrrhizae, radix sophorae flavescentis, eucommia, dipsacus root, fructus rubi, rhizoma atractylodis macrocephalae, radix rehmanniae, radix polygonati officinalis, rhizoma acori graminei, radix paeoniae rubra, sinapis alba, radix paeoniae alba, glossy privet fruit, fritillaria thunbergii miq, cuttlebone, notopterygium, hippocampus, astragalus membranaceus, pinellia ternate, cistanche salsa, rhizoma drynariae, cynomorium songaricum, cortex phellodendri, rhizoma atractylodis, radix rehmanniae preparata, dendrobium, salviae miltiorrhizae, cortex moutan, hawthorn, fructus cnidii, fatmelon kernel, dry longan, pericarpium citri reticulatae, wolfberry, lily and malt.

The invention relates to Lactobacillus reuteri DSM 17938 for promoting the establishment of healthy and normal cognitive function in young mammals. In particular, the invention relates to the use of Lactobacillus reuteri DSM 17938 for promoting development of the brain structures responsible for cognitive functions (e.g. cortex and hippocampus) and its associated neural pathways and / or to reverse retardation and / or to prevent retardation of the establishment of cognitive functions. Humans or animals and, in particular, a foetus, pre-term or term born infant, toddler or child or a young adult may benefit from the invention.

Adult neural stem cells differentiate into neurons, astrocytes, and oligodendrocytes in the mammalian CNS, but the molecular mechanisms that control their differentiation are not yet well understood. Insulin-like growth factor-I (IGF-I) can promote the differentiation of cells already committed to an oligodendroglial lineage during development. However, it is unclear whether IGF-I affects multipotent neural stem cells. Here we show that IGF-I stimulates the differentiation of multipotent adult rat hippocampus-derived neural progenitor cells into oligodendrocytes. Modeling analysis indicates that the actions of IGF-I are instructive. Oligodendrocyte differentiation by IGF-I appears to be mediated through an inhibition of BMP signaling. Furthermore, overexpression of IGF-I in the hippocampus leads to an increase in oligodendrocyte markers. These data demonstrate the existence of a single molecule, IGF-I, that can influence the fate choice of multipotent adult neural progenitor cells to an oligodendroglial lineage.

The invention discloses a hierarchical cultivation method for seahorse seedlings. The primary cultivation is carried out in a glass box filled with light green seedling-raising water. Artemia larvae are firstly fed, and then 80-mesh copepods are used for feeding. The seahorse seedlings The growth is good, and the seedlings are cultivated to a length of more than 15mm; the second, third, fourth and fifth grades are cultivated in indoor nursery ponds with light green seedling water, and the bait for the second grade cultivation is copepods over 80 mesh, which is conducive to feeding and growth , cultivated until the seedling body length is more than 25mm, the bait for the third-level cultivation is mysis or shrimp seedlings, the third-level cultivation is until the seedling body length is more than 40mm, and the fourth-level cultivation starts to domesticate and feed the bait by gradually increasing the frozen shrimp, and cultivate to the seedlings The body length is more than 55 mm, and no mysis or shrimp seedlings are added at the end of the five-level cultivation, and the frozen shrimp is completely fed, and the seedlings are cultivated to a body length of more than 70 mm; the hippocampus seedlings cultivated by the method of the present invention have neat specifications and good adaptability , a higher growth rate.

The invention relates to the field of medicine technology and provides an injection for curing ischemic-reperfusion damage of brain, heart, kidney and other tissue organs. The preparation method includes placing a medical injection flexible bag under low pressure for degasification, carrying out low-temperature pretreatment after air extraction, then injecting hydrogen for pressurized dissolution and dissolving hydrogen in the injection and storing under constant pressure and about 4 DEG C for standby. The injection can be used after 24-hour stabilization. The animal experiment proves that the injection of the invention can reduce focal cerebral ischemic-reperfusion damage in rats, neuron injuries and apoptosis number, decrease the activity of apoptosis enzyme in pallium and hippocampus, reduce the ischemic-reperfusion damage extent of heart and kidney and protect the function of ischemic-reperfusion heart and kidney. The hydrogen-included injection can be used as the medicine for curing the ischemic-reperfusion damages of brain, heart, kidney and other important organs.

An objective of the present invention is to provide a safe and effective vaccine therapy for Alzheimer's disease. A minus strand RNA viral vector carrying amyloid gene was constructed, and administered intranasally to 24- to 25-months-old APP transgenic mice. The level of serum anti-A 42 antibody was determined and showed to be markedly higher than the control. The results of histological investigation showed that the administration of a vector of the present invention markedly reduced senile plaques in all of the frontal lobe, parietal lobe, and hippocampus. The brain A level was also markedly reduced. Furthermore, the administration of a vector of the present invention did not result in lymphocyte infiltration in the central nervous system.

The invention discloses a preparation method of a hippocampus polypeptide enzyme solution. The preparation method is characterized by comprising the steps of: a, mixing hippocampus bone powder which is degreased by virtue of a CO2 supercritical fluid extraction technology and water according to a proportion of 40-60g / L, adding the mixture into a glass vessel, arranging the glass vessel into a water bath boiler at the temperature of 40-50 DEG C, carrying out heat preservation for 8-12 minutes, adding trypsin into the mixture according to a proportion of 2-4 percent, regulating a pH value to 8.5-9, carrying out enzymatic hydrolysis for 4-6 hours, and carrying out enzyme inactivation for 10 minutes; and b, continuously arranging the glass vessel after enzyme inactivation into the water bath boiler at the temperature of 50-60 DEG C, preserving the temperature for a period of time, adding 2-4 percent of alkaline protease into the glass vessel, regulating the pH value to 8.5-9.5, carrying out enzymatic hydrolysis for 5-7 hours, carrying out enzyme inactivation for 10 minutes, carrying out centrifugation for 10 minutes under the conditions of 5000r / min, and taking a supernatant to obtain the hippocampus polypeptide enzyme solution. The preparation method has the advantages of simple technology and convenience for operation; and in addition, the prepared polypeptide is high in hydrolysis degree, smaller in polypeptide fragment molecular weight and is easily digested and absorbed by a human body.

The invention discloses a method for breeding hippocampus by using a stratified net cage. The method uses the independently designed multiple-layer net cage to meet the necessary conditions of the hippocampus breeding and realize large-scale breeding of hippocampus. The method can realize the composite breeding of hippocampal seedlings, middle-aged hippocampus and parent hippocampus; the breakthrough breeding mode can reduce the technology pressures of hippocampus food supply and inflatable water body to the greatest degree, and greatly reduces the hippocampus breeding cost; at the same time, due to the very stable breeding environment and conditions, the accidental mortality of the hippocampus is reduced to a certain degree.

The invention belongs to the technical field of medical image processing, and discloses a hippocampus segmentation method for an automatic brain MRI (Magnetic Resonance Image) on the basis of multipleatlases. The method comprises the following steps that: (1) adopting a non-rigid registration method to carry out registration on atlas set and a brain MRI to be segmented; (2) calculating a similarity between an atlas image and a target image, and constructing and selecting a similar atlas which is most favorable for target image hippocampus segmentation; and (3) obtaining the confidence coefficient weighting probability matrix of the atlas image, establishing a context model based on the similar atlas, and combining the confidence coefficient weighting probability matrix of the atlas imagewith the context model to obtain a hippocampus segmentation result in the target image. By use of the method, image features and image used for segmenting the hippocampus in the atlas image can be mined, an accurate hippocampus segmentation result can be obtained under a situation that time complexity is controlled, and the problem in the prior art that the automatic segmentation accuracy of the hippocampus for the brain MRI image is low is overcome.

The invention relates to the field of computer vision and deep learning, in particular to a hippocampus segmentation method based on sequence learning. The method comprises the following steps: step 1, preprocessing an original image set A; 2, building a network model, wherein the hippocampus segmentation network model comprises an encoding part, a bidirectional convolution long and short memory network and a decoding part; Step 3, a model is trained; And performing forward propagation on the anatomical plane image set D, E and F to obtain a single iteration result, and calculating a loss function to obtain weight models J, K and L through backward propagation. According to the method, the hippocampus structure in the human brain nuclear magnetic resonance image is efficiently, automatically and accurately segmented by utilizing the method based on the deep learning network, and the operation speed is relatively high while the high segmentation precision is ensured. Besides detection of hippocampus, the network provided by the invention can be retrained, so that the network can be applied to detection and segmentation of other organs or tissues.

The invention relates to a multi-graph image segmentation based on direction and scale descriptors. The method comprises the steps of reading all graph gray images Itrain and corresponding graph mark images L, reading an image Itarget to be segmented, taking the image to be segmented as a reference image and the graph gray images as floating images, registering all graph gray images Itrain to a target image one by one to obtain the deformation fields T (T=(T1, T2 to Tk)) of graph gray image deformation and deformed graph gray images I'train, then allowing the deformation fields to act on corresponding graph mark images, obtaining deformed graph mark images L', using L' prior information, determining a hippocampus dissection position in the image to be segmented, determining an interest area, and calculating the direction and scale descriptors S of all pixels of the graph gray images and target pixels, wherein the pixels in the interest area are the target pixels, and the direction and scale descriptors S are new pixel characteristics. According to the method, the segmentation error caused by uneven brightness between graphs can be reduced, and the accuracy of segmentation is improved.

The invention relates to a fish bait feeding method for hippocampus erectus adults, which comprises the followings: bait feeding to hippocampus erectus is performed under the condition of an allocating culture system and breeding environment. The method specifically comprises the followings: A, normal cultivation period: water temperature is controlled within 25 to 28 DEG C, artificial light is adopted for prolonging photoperiod for 0.5 to 1 hour before sunrise and after sunset, bait is fed according to 12 to 15 percent of weight, and feeding is performed for three times respectively; siphon of manure and residuary bait are respectively performed; and B, overwintering period: water temperature is controlled within 22 to 23 DEG C, artificial light is adopted for prolonging the photoperiod for 0.5 to 1 hour before sunrise and after sunset, bait is feed according to 8 to 10 percent of weight and feeding is performed for three times respectively; and siphon of manure and residue bait are respectively performed. According to the invention, artificial light is adopted for prolonging photoperiod, defecation of hippocampus is accelerated, the opportunities of water pollution and the accessions of gas disease are reduced, and the sickness rate of gas disease of hippocampus is generally lower than 5 percent. Therefore, the survival rate is increased and the application prospect is excellent.

The tripeptide glycine-proline-glutamine (GPE) may be administered before, or usually after, injury to reduce damage to the central nervous system. GPE appears useful for neuronal rescue particularly but not exclusively within the hippocampus. Advantages of GPE include: (a) that it crosses the blood-brain barrier, so is effective by injected peripheral administration, (b) it is unlikely to challenge the immune system, (c) it is cheap, and (d) its therapeutic ratio is high. GPE may also be infused into the CSF. It may be administered prior to parturition or elective brain or cardiac surgery. Transdermal routes may be useful for chronic neural disorders. The CNS of mammals (including foetal mammals) after trauma including hypoxic / ischaemic experimental insults showed reduced damage under GPE protection as measured by histological assessment of cell damage or death and regional shrinkage.

Disclosed is a Chinese medicine composite for treating oligozoospermia and astenozoospermia, comprising the following active pharmaceutical ingredients in portion by weight: 3.0-7.0 portions of parched semen cuscutae, 1.5-5.0 portions of herba epimedii, 1.0-3.0 portions of rhizoma curculiginis, 0.2-2.0 portions of hippocampus and 0.3-1.0 portions of chilopod. The Chinese medicine composite can be decocted in water or made into oral liquid, powder, capsule, tablet, honeyed pill, water-paste pill and other dosage forms for administration. The therapeutic result shows that the Chinese medicine composite can effectively promote the gonepoiesis, increase the motility rate and enhance the sperm motility. The Chinese medicine composite is a new prescription for treating the oligozoospermia and the astenozoospermia due to various causes, in particular to idiopathic oligozoospermia or astenozoospermia, which is simple, safe, convenient and active. The clinical cure rate and the total effective rate in the treatment group are respectively 50.90 per cent and 92.73 per cent.

The invention relates to a navigation method based on bionic hippocampus cognitive map, which belongs to the field of bionic mobile robot navigation. A cognitive map belongs to a topology map, each node of the cognitive map comprises biological characteristic information, environment RGB-D information, and association information of multiple interdependent nodes. By combining with the cognitive map, a Dijkstra algorithm is used, through an analysis, the various information in each node and a target-heading physical distance are subjected to weighted sum, the algorithm is improved, and a globalpath with maximum characteristic information and shortest physical distance can be obtained. According to inflection point numbers, the node with the most abundant characteristic surrounding the inflection point is found by the local navigation as a terminal point of the local navigation, through SURF image characteristic point extraction, a translation matrix and a rotation matrix between two frames are compared, and the error correction is carried out. The global navigation is segmented to multiple segments, the local navigation is carried out, and the accumulation error in continuous navigation can be reduced. The bionic hippocampus cognitive map can be effectively used in a practical navigation process, and a good utility effect can be obtained.