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AD model rat hippocampus gene expression difference chart constructed by combining A beta and D-gal as well as determination method and application of AD model rat hippocampus gene expression difference chart

A technology for gene expression differences and determination methods, which is applied in biochemical equipment and methods, microbial determination/inspection, genomics, etc., and can solve problems such as inability to accurately represent changes in vivo and unstable induction effect

Inactive Publication Date: 2018-09-28
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the model used is a cell model, and in vitro experiments cannot accurately represent changes in vivo, and Aβ is used alone 25-35 The induction effect is unstable

Method used

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  • AD model rat hippocampus gene expression difference chart constructed by combining A beta and D-gal as well as determination method and application of AD model rat hippocampus gene expression difference chart
  • AD model rat hippocampus gene expression difference chart constructed by combining A beta and D-gal as well as determination method and application of AD model rat hippocampus gene expression difference chart
  • AD model rat hippocampus gene expression difference chart constructed by combining A beta and D-gal as well as determination method and application of AD model rat hippocampus gene expression difference chart

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] (1) Establish AD model mice: the flow chart of establishment is as follows Figure 1-A Rats were injected intracerebroventricularly with 2 μl of aged amyloid peptide Aβ at a concentration of 1 μg / μl as indicated. 25-35 ; Subsequent treatment with 1 × 104U / day of penicillin dosage for 3 days; two weeks later, subcutaneous injection of D-galactose in rats with a dosage of 100mg and a concentration of 100mg / kg every day for 50 days;

[0066] (2) Behavioral characterization analysis: 14 days after the establishment of AD model mice, randomly put several AD model mice into the Morris water maze, and place the AD model mice on the hidden platform within 60 seconds. If the platform is not found within the period, it will be guided to the platform and stay for 10s; after an interval of 8 minutes, the AD model mice will be put back into the Morris water maze; continuous training for 3 days, 3 times a day, while recording the AD model mice from The time to escape to the platform ...

Embodiment 2

[0078] (1) Establish AD model mice: Inject 10 μl of aged amyloid peptide Aβ at a concentration of 10 μg / μl into the rat ventricle 25-35 ; Subsequent treatment with 8 × 104U / day of penicillin dosage for 5 days; two weeks later, subcutaneous injection of D-galactose in rats with a dosage of 200mg and a concentration of 200mg / kg every day for 50 days;

[0079] (2) Behavioral characterization analysis: 14 days after the establishment of AD model mice, randomly put several AD model mice into the Morris water maze, and place the AD model mice on the hidden platform within 60 seconds. If the platform is not found within the period, it will be guided to the platform and stay for 20s; after an interval of 15min, the AD model mice will be put back into the Morris water maze; continuous training for 7 days, 5 times a day, while recording the AD model mice from The time to escape to the platform in the Morris water maze;

[0080] Subsequently, the platform is removed from the Morris wate...

Embodiment 3

[0088] (1) Establish AD model mice: Inject 6 μl of aging amyloid peptide Aβ at a concentration of 5 μg / μl into the rat ventricle 25-35 ; Subsequent treatment with 4.5 × 104U / day of penicillin dosage for 4 days; two weeks later, subcutaneous injection of D-galactose in rats with a dosage of 150mg and a concentration of 150mg / kg every day for 50 days;

[0089] (2) Behavioral characterization analysis: 14 days after the establishment of AD model mice, randomly put several AD model mice into the Morris water maze, and place the AD model mice on the hidden platform within 60 seconds. If the platform is not found within the period, it will be guided to the platform and stay for 15s; after an interval of 12min, the AD model mice will be put back into the Morris water maze; continuous training for 5 days, 4 times a day, while recording the AD model mice from The time to escape to the platform in the Morris water maze;

[0090] Subsequently, the platform is removed from the Morris wat...

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Abstract

The invention relates to a construction method of an AD model rat hippocampus difference gene expression chart by combining Abeta and D-gal. The construction method comprises the following steps: firstly, treating a rat with the Abeta and the D-gal to establish an AD model rat; secondly, measuring memory damage of the AD model rat by using a Morris water maze so as to determine successful construction of the AD model rat; thirdly, obtaining hippocampus samples of an AD model group and a normal control group, and respectively obtaining gene chip data of all tested hippocampus gene expressions;fourthly, uploading all the gene chip data to gene chip data analysis software, obtaining differential expression genes and screening a gene spectrum of the differential expressions of the AD model rat; fifthly, carrying out functional annotation classification and a biological pathway analysis on the differently-expressed genes to reveal molecular basis associated with the AD. According to the construction method disclosed by the invention, the Abeta and the D-gal are used as a whole AD model to study the gene expression spectrum of the AD model rat hippocampus genes, so that other errors changing the expressions and induced by exogenous genes are effectively avoided.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for determining the difference spectrum of gene expression in the hippocampus of AD model mice, in particular to a method for combining A β The difference profile of gene expression in the hippocampus of AD model mice constructed with D-gal and its measurement method and application. Background technique [0002] Alzheimer's disease (AD) is the most prevalent age-related progressive neurodegenerative disease characterized by cognitive decline and memory deficits, accounting for about 2 / 3 of all elderly patients with cognitive impairment. Although AD has a devastating impact on the behavior of patients and ultimately poses a serious threat to their lives, clinical evidence based on the presence of intracellular neurofibrillary tangles (NFTs) in the cerebral cortex and extracellular amyloid plaques (APs) in the hippocampus Pathological diagnosis is only applicable to mo...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883G06F19/18A01K67/027
CPCA01K67/027A01K2207/10A01K2207/20A01K2227/105A01K2267/0312C12Q1/6883C12Q2600/156G16B20/00
Inventor 黄飞娟吴正治秦鉴
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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