The invention relates to the bio-technical field, and discloses a cultural method for retina progenitor cells and a culture medium of the retina progenitor cells. According to the cultural method for cells disclosed by the invention, anchorage-dependent cells or suspension cells grow in an extracellular matrix with a certain three-dimensional space, so that so as to the growth velocity of cells is prevented effectively, cells are prevented from being aged or differentiated, the gene expression of the cells is not changed, high-quality and high-density cell reproduction is guaranteed and limitations of low yield of cells, poor quality of cells and the like in the traditional cultural method can be broken, so that the cultural method has a wide application prospect.