151 results about "Retinal pigment epithelium" patented technology

The invention discloses a vessel-based automatic registration method for an eye fundus image and a spectral-domain optical coherence tomography (SD-OCT) projection image, belonging to the technical field of image processing. The method comprises the following steps: performing tissue layer segmentation on an input SD-OCT image, locating the upper boundary of a photoreceptor inner-segment / outer segment (IS-OS) layer and the lower boundary of a retinal pigment epithelium (RPE), generating an SD-OCT projection image according to the upper boundary of the IS-OS layer and the lower boundary of an RPE, and segmenting the SD-OCT projection image by using an improved level set method; segmenting a colored eye fundus image by using a morphology multi-scale top hat method; searching for the local maximum value of a maximum similar function by using an iteration blocking method to extract a control point, and calculating an affine variation coefficient according to the control point to realize registration of the SD-OCT projection vessel image and the colored eye fundus image. As proved by an experimental result, the image registration accuracy is high in different modes.

The invention relates to a method for identification of substances which are applicable for treatment or prevention of an insufficient longitudinal growth of the eye (hypermetropia) or for treatment or prevention of an excessive longitudinal growth of the eye (myopia); substances identified by the method for treating or preventing conditions related to the longitudinal growth of the eye; substances and mixtures of substances for the preparation of a pharmaceutical composition for the treatment or prevention of abnormal growth of the axial length of the eye. The identification involves measuring the effect of the substances on the retinal pigment epithelium of the eye, e.g. by detecting the metabolic effect of the substance on the retinal epithelium, the effect on the standing potential or the effect on the proteoglycanes of the scleral tissue of the eye, by way of EOG examination, by way on the size of the so-called c-wave in ERG-recordings, or by the state of the Ca<2+>-channels or on the [<3>H]-ryanodine receptors of the retinal pigment epithelium.

This invention relates to methods for improved cell-based therapies for retinal degeneration and for differentiating human embryonic stem cells and human embryo-derived into retinal pigment epithelium (RPE) cells and other retinal progenitor cells.

This invention relates to methods for improved cell-based therapies for retinal degeneration and for differentiating human embryonic stem cells and human embryo-derived into retinal pigment epithelium (RPE) cells and other retinal progenitor cells.

Geographic atrophy of the eye can be detected and measured by imaging the eye at a depth greater than the retinal pigment epithelium (RPE) at a plurality of locations of the eye, for example, using optical coherence tomography (OCT); determining a ratio of the intensities of imaging signals of a retinal layer(s) with respect to the intensity of imaging signals of a sub-RPE layer(s) at each location; determining representative values based at least in part on the determined ratios; generating a map of the representative values; and identifying diseased areas from the map. Contours and binary maps may be generated based on the identified diseased areas. The size and shape of the identified areas may be analyzed and monitored over a period of time.

An image processing apparatus including a storage unit configured to store tomographic images in relation to fundus of an examined eye; a detection unit configured to detect a boundary of retinal pigment epithelium and an inner limiting membrane from each of the images and to detect a part where the boundary is discontinuous; a determination unit configured to determine a surface of a sclera model for each of the images by use of the boundary and the inner limiting membrane; a generation unit configured to generate a sclera model including an optic papilla periphery by use of the surface of the sclera model and the part where the boundary is discontinuous; a combining unit configured to combine each of the images and the sclera model to generate a combined image; and a display unit configured to display the combined image generated by the combining unit, is provided.

The invention discloses a method for preparing retinal pigment epithelia. According to the method, stem cells are induced by utilizing a liver X receptor (LXR) activating agent to be differentiated into the retinal pigment epithelia. Compared with the prior art, the method for inducing the stem cells to be differentiated into the retinal pigment epithelia by utilizing the LXR receptor activating agent has the advantages that: a feed layer is not used, so that potential risks caused by an animal source feed layer are avoided; and the differentiation efficiency can be improved, and the differentiation time can be shortened.

The present invention provides devices and methods for applying radiation to the retina of a patient. In one embodiment, an apparatus includes a radiation source for generating a radiation beam suitable for absorption by retinal pigment epithelial cells. One or more optical components are included to direct the beam onto the retina. A scanner is optically coupled to the radiation source to control movement of the beam in two dimensions to allow a scan over the retina. A controller applies control signals to the scanner to adjust beam movement to illuminate a plurality of retinal locations in a temporal sequence according to a predefined pattern. The device can be operated in one mode to effect selective targeting of retinal pigment epithelial cells, or in another mode to effect thermal photocoagulation of the retina.

Provided herein are methods of enriching a retinal pigment epithelium (RPE) cell population derived from stem cells. Such a method may comprise removing contaminating cells through the depletion of CD24 positive cells, CD56 positive cells, and / or CD90 positive cells from a starting population of RPE cells.

The production of high quality retinal pigmented epithelium (RPE) cells is necessary for research and potential therapeutic uses. Especially desirable are methods for the production of RPE cells using xeno-free culture conditions. Disclosed herein are novel methods for the production of RPE cells from pluripotent cells with high yields, including xeno-free production methods. Also provided are methods of efficiently isolating RPE cells from cultures containing heterogeneous cell types, allowing for substantially pure RPE cell cultures to be established. Additionally, novel methods for the cryopreservation of RPE cells are provided.

An image processing apparatus acquires a tomographic image of an eye to be examined. The image processing apparatus quantifies a distortion in a region determined from the tomographic image. The region includes a photoreceptor layer or a retinal pigment epithelium.

Surgical instruments, surgical techniques, retinal cell grafts, retinal cell and tissue isolation techniques, and a method for transplanting retinal cells, including photoreceptors, and / or retinal pigment epithelium, with the cells in the isolated tissue having their normal cell to cell configuration are disclosed.

The invention provides a culture solution for retinal pigment epitheliums (hESC-RPE). The culture solution is characterized in that the culture solution contains, 96-97.5 volume fractions of a basic culture medium, 1 volume fraction of non-essential amino acids (NEAA), 1 volume fraction of L-glutamine, 0.5-21 volume fractions of an N2 additive, a component a and a component b. The invention further provides a preparation method of the culture solution and a method for culturing the retinal pigment epitheliums (hESC-RPE) by using the culture solution. Compared with existing culture media, the culture medium of the culture solution is simple composition, the costs are lower, no ethical problem is related, and cell proliferation can be promoted.