The invention discloses a method for hydroponically culturing lettuce by means of an LED light source. The method is characterized by comprising the following steps that 1, after lettuce seeds are germinated, seedling raising is conducted, and hydroponic culturing is conducted in a nutritional solution till seedlings growing out 3-4 true leaves are obtained; 2, transplanting and field planting are conducted on the seedlings; 3, after transplanting, light treatment is conducted on the seedlings, and the lettuce seedlings with the 3-4 true leaves are placed under an LED combining red light, blue light and green light to be cultured for 30 days to 33 days, wherein the wave length of the red light ranges from 655 nm to 660 nm, the wave length of the blue light ranges from 455 nm to 460 nm, the wave length of the green light ranges from 520 nm to 525 nm, the light intensity is 400 mumol.m<-2>.s<-1>, and the effective photosynthetic light quantum density percentage ratio of the red light to the blue light to the green light is 36:24:40. According to the method, a red-blue-green LED light control console is adopted, a specific proportion of green light is added in the red light and the blue light on the condition that the total light intensity keeps consistent, therefore, the lettuce which is high in yield, good in quality and high in beneficial mineral element content can be produced, and high-quality and efficient production of the hydroponically-cultured lettuce is achieved.