232 results about "Glutathione peroxidase" patented technology

A novel strain of lactic acid bacteria was found to be heat resistant and able to grow in a sulfur-limiting medium (SLM) containing a high concentration of sodium selenite. The microorganism is a non-spore forming and Gram-positive coccus, which is identified with >90% confidence using the API biochemical and sugar fermentation tests, ribotyoing and 16S rRNA sequencing as Pediococcus pentosaceus SP80. In the current study, P. pentosaceus SP80 grown on SLM containing 250 ppm sodium selenite produced both organic and inorganic forms of selenium. These selenium compounds can be separated using an anion exchange chromatography technique. The concentrations of selenium detected in the organic and inorganic fractions were 4.34 and 21.7 ppm, respectively. Selenium-enriched bacteria are useful as a source of selenium for supplementing the diets of animals and humans. Animals fed efficacious amounts of the selenium-enriched bacteria show improved feed conversion rates and higher levels of glutathione peroxidase (GPx) activity in heart, kidney and liver tissues indicating an increased absorption and retention of selenium over control diets.

A process for preparing the oligose of fucosan sulfate includes such steps as preparing the aqueous solution of fucosan sulfate, adding oxidant chosen from hydrogen peroxide, hypochlorous acid, nitrous acid, or their salts, heating, membrane ultrafilter, vacuum concentrating and freeze drying. Its advantages are high medical effect to protect liver and take care of health, and low cost.

The present invention relates to the use of a mitochondrially targeted antioxidant, e.g. derivatives of vitamin E, coenzyme Q10 or a glutathione peroxidase mimetic, in the treatment and prevention of liver diseases and / or epithelial cancers. The present invention also relates to pharmaceutical compositions containing the antioxidant(s) intended for such use. Furthermore the invention relates to the manufacture of medicaments containing the antioxidant(s) useful for such prevention and treatment.

The present invention relates to a method for identifying and / or validating candidate substances for the treatment of Friedreich Ataxia (FRDA). Furthermore, the present invention relates to the use of selenium, Ebselen and Glutathione peroxidase (GPX) mimetics for the preparation of a medicament for the treatment of FRDA. Another aspect of the present invention relates to the use of cells with reduced frataxin expression for identifying and / or validating candidate substances for the treatment of Friedreich Ataxia.

The invention discloses an amino acid compound fertilizer, a preparation method and an agriculture ecological cycle development method. The amino acid compound fertilizer is prepared from the following raw materials: amino acid solution, copper sulfate, zinc sulfate, magnesium sulfate, ferrous sulfate, manganese sulfate, ammonium molybdate, sodium selenite, borax, sodium nitrophenolate, citrate, metasilicate or silicate, and urea. According to the amino acid compound fertilizer, the amino acid or micromolecular active protein in the amino acid solution are chelate with various essential elements for plant, cotton wilt disease can be effectively prevented and controlled, the restoration of crops which suffer from chilling damage or hails can be promoted, and the crops with toxicity due to excessively applied elements and herbicide toxicity can be effectively detoxified; functionalized health-care crop products rich in selenium, silicon, glutathione peroxidase, superoxide dismutase, peroxidase and catalase can be harvested.

The present invention relates to a compound of Antrodia camphorata used for liver protection, in particular to an extract, 4-hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone which is isolated from Antrodia camphorata. The cyclohexenone compound according to the invention helps to alleviate liver injury and fibrosis induced by chemicals and reduces the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). By increasing the contents of glutathione peroxidase (GSHPx) and catalase (CAT), cyclohexenone further decreases the liver damage and the oxidative pressure caused by free radicals, enhances the antioxidant ability and achieves the purposed of liver protection.

The invention belongs to the technical field of biological monitoring environment, and specifically relates to a shellfish monitoring method for ocean oil spill pollution. The shellfish monitoring method comprises the steps of utilizing typical bivalve mollusk such as short-necked clam and chlamys farreri in China to determine the shellfish visceral mass autioxidant enzyme activities including superoxide dismutase, glutathione glutathione S-transferase, peroxidase, glutathione peroxidase and catalase activity, integrating the enzyme activities so as to form an integration biomarker responding index (IBR), establishing a dose-effect relationship between the IBNNR and petroleum concentration, adopting a one-dimensional variance components method (ANOVA) to carry out notable difference analysis, determining the forcing degree of petroleum pollution on organisms, and finally judging pollution level of the ocean oil spill.

Methods for treating cancer are provided including administering a patient needing treatment a therapeutically effective amount of one or more antioxidants selected from the group of catalase, N-acetylcysteine, glutathione peroxidase, salen-transition metal complexes, dicumarol, and derivatives thereof.

The invention discloses lactobacillus gasseri and application thereof in relieving and treating hyperuricemia, and belongs to the technical field of microorganisms. According to the lactobacillus gasseri CCFM1133 disclosed by the invention, the serum uric acid level of hyperuricemia mice and the xanthine oxidase (XOD) activity of serum and a liver can be reduced, and the occurrence of hyperuricemia and gout is reduced; the blood glucose and serum triglyceride (TG) level of a patient with hyperuricemia can be regulated, and the activity of liver catalase (CAT) and glutathione peroxidase (GSH-Px) is improved; expression of ileum ABCG2 is improved, and excretion of intestinal uric acid is promoted; and the intestinal short-chain fatty acid level is improved, and the health is promoted. The lactobacillus gasseri CCFM1133 disclosed by the invention can be used for preparing foods, functional foods or medicines, and has a wide application prospect.

The present invention relates to the method of treatment or prophylaxis of patients with drug-induced liver diseases which comprises administering to the patient in need thereof a therapeutically effective amount of a mitochondrially targeted antioxidant compound e.g. derivatives of vitamin E, coenzyme Q10 or a glutathione peroxidase mimetic. The present invention also relates to pharmaceutical compositions containing the antioxidant(s) intended for such use.

This invention is directed to methods and compositions for the treatment of cardiovascular disorders. Specifically, the invention is directed to compositions comprising vitamin E, statins and / or glutathione peroxidase mimetics; methods of treating diabetic patients expressing the Hp-2-2 haptoglobin genotype; a method of inhibiting or suppressing a cardiovascular disorder in a diabetic subject, treating cardiovascular disease in subjects exhibiting the Haptoglobin Hp-2-2 genotype; and methods of treating cardiovascular disease in subjects exhibiting the Haptoglobin Hp-2-2 genotype.

The invention discloses an anti-oxidation active extract of citrus peels as well as an extraction process and application thereof. The active extract of the citrus peels mainly contains a citrus flavonoid substance and pectic polysaccharide. The extraction process of the active extract of the citrus peels comprises the steps of preprocessing raw materials, refluxing and extracting by a dipping method, decompressing and concentrating and drying in vacuum. The invention takes the in-vitro oxidation resistance of the extract to combine with the extraction yield as indexes, adopts a response surface method to optimize the extraction process, has reasonable technological conditions and good effect stability and is suitable for industrialized production. The active extract of the citrus peels, which is obtained by extraction, has an obvious effect to a model mouse with senility caused by D-galactose and can lower the content of the MDA (malondialdehyde) of the plasma, the liver and the brain obviously; the activity of the SOD (superoxide dismutase) of the plasma, the liver and the brain is obviously enhanced; the activity of the GSH-Px (glutathione peroxidase) of the plasma and the liver is obviously enhanced; and the invention can be used for preparing an anti-oxidation medicine, an anti-oxidation food or an anti-oxidation health-care product.

The present invention relates to a compound of Antrodia camphorata used for liver protection, in particular to an extract, 4-hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone which is isolated from Antrodia camphorate. The cyclohexenone compound according to the invention helps to alleviate liver injury and fibrosis induced by chemicals and reduces the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). By increasing the contents of glutathione peroxidase (GSHPX) and catalase (CAT), cyclohexenone further decreases the liver damage and the oxidative pressure caused by free radicals, enhances the antioxidant ability and achieves the purposed of liver protection.

The present invention relates to a method for investigating body fluids for cancer cells, the use thereof and corresponding analysis kits, and the possibilities for cancer treatment derived therefrom. The method is based essentially on determining the expression of the manganese superoxide dismutase, thioredoxin reductase and / or glutathione peroxidase genes. Use of this method permits in particular reliable tumor diagnosis and prognosis. Diminishing an elevated expression of these genes has therapeutic value and may be utilized for cancer treatment.

This invention relates to compositions and methods for the treatment of dermatological conditions with a glutathione peroxidase mimetic.

The invention belongs to the technical field of biology, relating to a modified glutathione peroxidase and a preparation method thereof. The preparation method comprises the following steps of: reacting single-chain monomethoxypolyethylene glycol (mPEG1) with amino-groups of glutathione peroxidase through free chlorine atoms so as to connect the mPEG1 to the glutathione peroxidase, wherein the activated single-chain mPEG1 is used as a modifier to react with the glutathione peroxidase at 4-37 DEG C with a pH value of 7.0-10.0, and the mass ratio of the single-chain mPEG1 to the glutathione peroxidase is (20-100):1; adding glutathione (GSH) by a proportion of 1:1 of the modifier to the GSH used as a terminator to terminate the reaction after reacting for 15-60min; and then removing the terminator by using 30 thousands of ultrafiltration membranes for ultrafiltering to obtain the modified glutathione peroxidase. The amino-group modification rate of the modified glutathione peroxidase is controlled to be 40-45 percent, and the enzymatic activity retention rate exceeds 100 percent.

The invention relates to a compound Chinese medicinal preparation with antifatigue and antioxidation effects, and a preparation method thereof. The compound Chinese medicinal preparation is characterized by comprising the following components in part by weight: 2 to 10 parts of manyflower solomonseal rhizome, 3 to 10 parts of astragalus, 1 to 5 parts of American ginseng, 1 to 3 parts of lucid ganoderma, 0.5 to 1.5 parts of cordyceps sinensis, and 0.5 to 1.5 parts of cornu cervi pantotrichum. According to a formula, the medicines can be prepared into various formulations. Through the administration experiment of aging mice and crowd, compound Chinese medicinal preparation oral liquid can obviously improve the content of superoxide dismutase (SOD) and glutathione (GSH-Px) of an organism, has an effect of resisting and removing free radicals of the organism and delays the aging process; and by observing time of swimming with a load attached to the body of mice, SOD and the content of malondialdehyde (MDA), large and middle doses of the Chinese medicinal preparation can prolong the time of swimming with the load attached to the body of mice, improve the SOD activity of serum, and reduce MDA value, so that the compound Chinese medicinal preparation can enhance the antifatigue and antioxidation effects of mice by resisting free radicals.

The present invention provides a method for diagnosing a disease state associated with oxidative stress in a subject. The method includes the step of measuring the level of non-selenium glutathione peroxidase protein in a biological fluid or tissue obtained from the subject over time to detect an increase in the level of non-selenium glutathione peroxidase protein in the subject and / or measuring the level of non-selenium glutathione peroxidase protein in a biological fluid or tissue obtained from the subject and comparing the measured level of non-selenium glutathione peroxidase protein with a control level.

The invention relates to traditional Chinese medicine composition with functions of relieving alcoholism and protecting the liver. The traditional Chinese medicine composition comprises raw medicinalmaterials as follows: flos puerariae, semen hoveniae, medicated leaven, fructus amomi rotundus, white poria cocos, radix ginseng, raw fructus crataegi, fructus schisandrae chinensis and raw oysters, and the raw medicinal materials are compatible and have the functions of eliminating dampness caused by alcohol and regulating qi-flowing for strengthening spleen. The pharmacological experiment indicates that with the adoption of the traditional Chinese medicine composition, drunkenness latency time of a mouse can be prolonged, drunkenness time of the mouse is shortened, content of alcohol in blood is reduced, level of ALT (alanine aminotransferase), AST (aspartate aminotransferase) and MDA (malondialdehyde) in the liver is reduced, activity of GSH-PX (glutathione peroxidase) and ADH (alcoholdehydrogenase) is improved, and the traditional Chinese medicine composition has excellent effects of relieving alcoholism and protecting the liver.

The invention relates to a red special diet instant drink for females and a processing method, and belongs to the technical field of the manufacturing of modern functional food. The processing method comprising the following steps of: performing combined energy-saving and quality-guaranteeing drying of hot pump-microwave-hot air on natural, safe, green and non-pollution grain, fruits, vegetables and algae which serve as raw materials, crushing to form 80 to 120-mesh powder, and blending and mixing uniformly according to the requirement to prepare health nutritional instant drink products which can be instant to drink. The red special diet instant drink satisfies the demands for daily energy of human bodies to a maximum degree, reserves functional factors such as bioactivity polysaccharide, superoxide dismutase, glutathione peroxidase, catalase, flavone, vitamins, and has the health-care functions of slimming, strengthening bodies, maintaining beauty and keeping young, delaying aging, regulating immunity and the like on the females.

The invention relates to the technical field of healthcare food, in particular to a healthcare food with antioxidant function. The healthcare food is made by 50-150 parts of haematococcus pluvialis, 50-150 parts of grate skin extracts, 10-100 parts of soya bean lecithin, 90 parts of safflower and 1.5 parts of oryzanol according to weight. Taking the healthcare food can increase content of SOD (superoxide dismutase), T-AOC (total-antioxidative capacity) and GSH-Px (glutathione peroxidase) in blood serum and decrease H2O2 content, and the healthcare food is remarkable in effect and capable of expelling in-vivo free radicals, has certain antioxidant function and can improve antioxidant ability in climacteric. Besides, the healthcare food is simple in recipe, easy to manufacture and low in side effect on humans.

The invention belongs to the field of biotechnology, and particularly relates to adipose tissue cryopreservation liquid, a cryopreservation method and application of the adipose tissue cryopreservation liquid. The adipose tissue cryopreservation liquid is prepared from a DMEM culture medium, sucrose, propylene glycol, proline, tetrahydromethyl pyrimidine, dismutase superoxide, glutathione peroxidase, and tocopherol. No serum is added into the prepared cryopreservation liquid, pathogenic microorganism pollution and rejection reaction are avoided, no DMSO with strong toxicity is added, and thustoxic adipose tissue iss are prevented from being cultured. The cryopreservation liquid has a good cryopreservation effect on the adipose tissues, the adipose tissue iss are high in cell activity andlow in apoptosis rate, a preparation method is simple and easy to operate, and new ideas can be provided for research and development of the field.

The invention provides an antioxidative animal bifidobacterium strain with the preservation number is CCTCC (China Center For Type Culture Collection) NO:M2013282. The animal bifidobacterium can be used for preparing pharmaceutics, food or health-care products. With the animal bifidobacterium, the enzymatic activity of superoxide dismutase, glutathione peroxidase and catalase in an organism can be obviously improved, the level of malondialdehyde in the organism can be lowered, so that the antioxidation capability of the organism can be effectively improved, the immunity of the organism can be enhanced, and the antioxidative animal bifidobacterium can be widely applied in pharmaceutics, food or health-care products.

The invention is a preparing process of novel organic selenium-methionine selenium, and its characteristic: the process of the methionine selenium product includes the working procedures of selenium dichloride synthesis, selenium tetrachloride synthesis, methionine salt synthesis, methionine selenium synthesis, drying, crushing, etc, and the novel methionine selenium has high biological utilization ratio, low toxicity, improves the oxidation resistance and immune function of the organism and regulates internal secretion by enhancing the activity of glutathione peroxidase in tissue cells and plays a remarkable role in oxidation resistance, immune efficiency of body fluid, promoting the growth of domestic fowls and animals, improving quality of muscles, etc.

The present invention discloses a muscle atrophy preventing and relieving health-care food for the elderly, which is characterized in that the health-care food comprises the following raw materials in mass percentages: 60-70% of soybean oligopeptides, 16-22% of compound sweeteners, 10-15% of xylitol, 1-5% of siraitia grosvenorii extract, and 0.08-0.15% of citrus sinensis essence. The soybean oligopeptides in the health-care food can improve the activities of superoxide dismutase and glutathione peroxidase, inhibit lipid peroxidation, scavenge hydroxyl radicals, help to reduce tissue oxidation, and protect human body. Besides, the soybean oligopeptides can instantly repair damaged skeletal muscle cells during exercise, maintain the integrity of the structure and function of the skeletal muscle cells. At the same time, the health-care food can increase the secretion of testosterone and promote protein synthesis, which are conductive for the prevention and relieving of the elderly muscle atrophy.

The invention provides an anti-alcoholism beverage using tea saponin as a main body. The anti-alcoholism beverage comprises the following ingredients: tea saponin, extracts from pueraria, extracts from pericarpium citri reticulatae, jujube date polysaccharide, extracts from medlar, extracts from haw apple, honey, citric acid, vitamin B1, vitamin B6 and vitamin C. The disclosed anti-alcoholism beverage is high in raw material utilization ratio, low in cost and good in taste, and can protect liver and stomach. According to the anti-alcoholism beverage provided by the invention, the drunkenness endurance time of mice can be prolonged, the sleeping time is reduced, the concentration of ethyl alcohol in blood can be obviously reduced, the activity of in vivo glutathione peroxidase and the activity of superoxide dismutase after drinking are increased, the metabolism of the ethyl alcohol and the elimination of free radicals in the liver are accelerated, the generation of peroxides is reduced, the increase of malondialdehyde content caused by alcohol is inhibited, the activity of ethanol dehydrogenase is further increased to a certain extent, the metabolism of the ethyl alcohol in the liver is quickened, the damage of the ethyl alcohol on human bodies is reduced, and the anti-alcoholism beverage has good taste.

The invention discloses application of donkey-hide gelatins to preparation of a medicament or healthcare product for treating respiratory injury caused by fine air particulate matters, and belongs to the field of novel medicinal uses of donkey-hide gelatins. SD rats continuously inhale the fine air particulate matters for 8 weeks, and are given by gavage with different dosages of donkey-hide gelatin solutions for medicinal intervention at the same time of contamination, researches on an injury mechanism caused by toxicity and a protection mechanism of the donkey-hide gelatins for respiratory injury caused by the fine air particulate patterns are made from weights, organ coefficients and pulmonary functions in combination with the aspects of the hematology, blood biochemical index measurement, respiratory functions, histopathological examination, oxidative damage and the like, and experimental results show that the donkey-hide gelatins can be used for effectively reducing the content of malondialdehyde and the number of pulmonary alveolar phagocytes and increasing the content of glutathione peroxidase, have an exact improvement effect on rat pulmonary function and pulmonary pathological changes caused by the fine air particulate matters and can be applied to preparation of the medicament or healthcare product for treating respiratory injury caused by the fine air particulate matters.

The present invention relates to a control technical system for important metabolic disease of dairy cattle after delivery, belonging to the technical field of veterinary medicine. The technical system is used for controlling and preventing important metabolic diseases of dairy cattle after delivery by detecting dairy cattle before and after delivery in the aspects of dietary cation anion balance (DCAB), oxidation resistance capability of cow plasma (glutathione peroxidase GSH-Px, superoxide dismutase SOD, methylene dioxyamphetamine MDA), plasma leptin hormone levels, liver function indexes (ornithine carbamoyl transferase OCT, aspartate transaminease AST, gamma-glutamyl transpeptidase GGT, total bilirubin Tbil, total protein TP, albumin ALB), blood biochemical indexes (acetone bodies, nonesterified fatty acid NEFA, lactic acid, blood sugar, blood fat, triglyceride TG, total cholesterol TC, calcium, phosphorus and magnesium), cow appetite, milk output increase speed, etc. The important metabolic diseases include cow parturient paresis, retention of afterbirth, endometritis, ketosis, ruminant acidosis, mastitis and fatty liver. The present invention is suitable for preventing and controlling metabolic diseases of dairy cattle after delivery in large-scale dairy farms.

The invention discloses an application of a mulberry polysaccharide extract in preparing a medicine or a health product capable of preventing or inhibiting chemical liver injury. The mulberry polysaccharide extract has relatively wide biological activity and high activity, and can obviously reduce the levels of alanine aminotransferase and aspartate transaminase in serum, effectively improve the activities of superoxide dismutase and glutathion peroxidase in the liver, and obviously reduce the content of glutathione and malonaldehyde. The mulberry polysaccharide extract can be used for preparing a medicine or a health product, in particular for preparing the medicine or the health product having an inhibiting or preventing effect for the chemical liver injury.

The invention discloses application of dicliptera chinensis polysaccharide. According to the application, the liver protecting and enzyme reducing activities of polysaccharide with different molecular weights are researched and summarized, particularly including application of a polysaccharide group with the molecular weight of less than 6000 and a total polysaccharide group to preparation of a medicament for treating TP (Total Protein) rise and / or SOD (Superoxide Dismutase) enzymatic activity reduction symptom caused by liver damage or health-care products, application of the polysaccharide group with the molecular weight of less than 6000 and the total polysaccharide group to preparation of a medicament for treating GSH-Px (Glutathione Peroxidase) enzymatic activity reduction symptom caused by liver damage or health-care products, and the like. According to the application disclosed by the invention, the phenomena of complex components and lower content of crude polysaccharides are improved and the relation between the molecular weight composition and the functions of polysaccharide is summarized. The dicliptera chinensis can be applied to preparation of a medicament for protecting liver and reducing enzyme. The product prepared by the invention is easy to dissolve and simultaneously retains the liver protecting physiologically activity of the dicliptera chinensis polysaccharide; and the product has high yield, high content and low preparation cost.