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Shellfish monitoring method for ocean oil spill pollution base on integration biomarker method

A biomarker and oil pollution technology, applied in the field of biological monitoring environment, can solve the problems of not being able to truly and comprehensively reflect the state of marine oil spill pollution, the accuracy of marine oil spill pollution is not high, long-term oil pollution cannot be detected, etc., to achieve Accurate assessment and prediction of risks and development trends, clear environmental characteristics, and small system errors

Inactive Publication Date: 2013-06-12
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0007] The purpose of the present invention is to overcome the low precision when using chemical analysis methods to detect marine oil spill pollution in the prior art, especially low-dose long-term oil pollution often cannot be detected, and cannot truly and comprehensively reflect the impact of marine oil spill on the marine environment. At the same time, the dose-effect relationship between single biomolecular indicators and oil pollution is not ideal, so as to provide an advanced, comprehensive and accurate biological monitoring method for marine oil spill pollution, using typical bivalve mollusks in my country such as Philippine clam , Chlamys farreri, measured the activity of antioxidant enzymes in shellfish viscera, and integrated these enzyme activities to form the Integrated Biomarker Response Index (IBR), and established the dose-effect relationship between IBR and oil concentration, using one-dimensional variance Analytical method (ANOVA) to conduct significant difference analysis to determine the degree of stress of oil pollution on organisms, and finally determine the pollution level of marine oil spills

Method used

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  • Shellfish monitoring method for ocean oil spill pollution base on integration biomarker method
  • Shellfish monitoring method for ocean oil spill pollution base on integration biomarker method
  • Shellfish monitoring method for ocean oil spill pollution base on integration biomarker method

Examples

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Embodiment 1

[0036] Embodiment 1: A method for monitoring shellfish polluted by marine oil spills based on the integrated biomarker method of the present invention adopts the following process steps:

[0037] The experimental subject organism, Philippine clam, was taken from the Hongdao Breeding Area of ​​Qingdao City. Wash them before the experiment, take them back and put them in the laboratory aquarium (130cm×50cm×60cm) for 5-7 days for later use. The water used in the experiment was natural seawater along the coast of Qingdao, which was treated by sand filtration. The salinity was 29.08±0.18, the pH was 7.85±0.03, and the temperature was (21.05±0.43)°C. During the temporary cultivation period, the water was continuously aerated and the water was changed every 24 hours. Feed Chlorella (3×10 6 individual / mL), feeding 2 times a day. During the holding period, the experimental shellfish had normal activities and were healthy and disease-free. Select 30 Philippine clams of uniform size a...

Embodiment 2

[0039] Embodiment 2: A method for monitoring shellfish polluted by marine oil spills based on the integrated biomarker method of the present invention adopts the following process steps:

[0040] The test organism Chlamys farreri was taken from Changdao breeding area in Shandong Province. Wash them before the experiment, take them back and put them in the laboratory aquarium (130cm×50cm×60cm) for 5-7 days for later use. The water used in the experiment was natural seawater along the coast of Changdao, treated by sand filtration, with a salinity of 29.25±0.18, a pH of 7.81±0.03, and a temperature of (21.21±0.43)°C. During the temporary cultivation period, the water was continuously inflated and changed every 24 hours. Feed Chlorella (3×10 6 individual / mL), feeding 2 times a day. During the holding period, the experimental shellfish had normal activities and were healthy and disease-free. Select 28 scallops of uniform size and specification (shell length: 4.9-5.1cm; shell hei...

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Abstract

The invention belongs to the technical field of biological monitoring environment, and specifically relates to a shellfish monitoring method for ocean oil spill pollution. The shellfish monitoring method comprises the steps of utilizing typical bivalve mollusk such as short-necked clam and chlamys farreri in China to determine the shellfish visceral mass autioxidant enzyme activities including superoxide dismutase, glutathione glutathione S-transferase, peroxidase, glutathione peroxidase and catalase activity, integrating the enzyme activities so as to form an integration biomarker responding index (IBR), establishing a dose-effect relationship between the IBNNR and petroleum concentration, adopting a one-dimensional variance components method (ANOVA) to carry out notable difference analysis, determining the forcing degree of petroleum pollution on organisms, and finally judging pollution level of the ocean oil spill.

Description

technical field [0001] The invention belongs to the technical field of biological monitoring environment, and in particular relates to a method for monitoring shellfish polluted by marine oil spills. Background technique [0002] The 21st century is the "century of the ocean". How to effectively protect the marine environment and maintain the ecological balance of coastal waters has become an important part of the national sustainable development strategy for each country. There are many ways to cause marine pollution, among which marine oil spill is considered to be one of the most serious and widespread types of marine pollution, which has attracted widespread attention from all countries in the world. In recent years, major oil spill accidents such as oil spills from oil fields and oil tankers have occurred frequently around the world, such as the 2010 Gulf of Mexico oil spill and the 2011 "Penglai 19-3" oil spill in the Bohai Sea. my country's marine environmental qual...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/48C12Q1/30C12Q1/28
Inventor 夏斌陈碧鹃辛福言孙雪梅崔毅
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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