48results about How to "Avoid cell damage" patented technology

An electrical current is created across an electrically conductive medium comprising a cell which may be part of a tissue of a living organism. A first electrical parameter which may be current, voltage, or electrical impedance is measured. A second electrical parameter which may be current, voltage or a combination of both is then adjusted and / or analyzed. Adjustments are carried out to facilitate analysis and / or obtain a desired degree of electroporation. Analysis is carried out to determine characteristics of the cell membrane and / or tissue.

Images created by electrical impedance tomography (EIT) are used to adjust one or more electrical parameters and obtain a desired degree of electroporation of cells in tissue. The parameters include current, voltage and a combination thereof. The cells are subjected to conditions such that they become permeabilized but are preferably not subjected to conditions which result in irreversible pore formation and cell death. The electroporation can analyze cell membranes, diagnose tissues and the patient as well as to move materials into and out of cells in a controlled manner.

A dietary supplement blend composition is disclosed, the basic formulation of the composition containing vitamins, minerals, and carotenoids. The composition can also contain bioflavonoids, cartilage protectors such as glucosamine or chondroitin, alpha-lipoic acid, coenzyme Q10, and a source of omega-3 fatty acids such as flax seed oil. The composition is beneficial for improving health and preventing disease, particularly for degenerative conditions. A method for supplementing the diet is also disclosed, wherein the quantity of daily rations of the dietary supplement blend composition is determined based on the person's age, body weight, and quality of diet.

Electroporation is performed in a controlled manner in either individual or multiple biological cells or biological tissue by monitoring the electrical impedance, defined herein as the ratio of current to voltage in the electroporation cell. The impedance detects the onset of electroporation in the biological cell(s), and this information is used to control the intensity and duration of the voltage to assure that electroporation has occurred without destroying the cell(s). This is applicable to electroporation in general. In addition, a particular method and apparatus are disclosed in which electroporation and / or mass transfer across a cell membrane are accomplished by securing a cell across an opening in a barrier between two chambers such that the cell closes the opening. The barrier is either electrically insulating, impermeable to the solute, or both, depending on whether pore formation, diffusive transport of the solute across the membrane, or both are sought. Electroporation is achieved by applying a voltage between the two chambers, and diffusive transport is achieved either by a difference in solute concentration between the liquids surrounding the cell and the cell interior or by a differential in concentration between the two chambers themselves. Electric current and diffusive transport are restricted to a flow path that passes through the opening.

A battery module with high thermal conductivity and its assembling method are disclosed. The battery module includes multiple battery cells, a primary retaining frame, a secondary retaining frame, common electrodes and a heatsink device. The primary and second retaining frames are combined together to constitute accommodation chambers for housing the battery cells and formed with interference flanged to secure the battery cells in position. Upon being inserted into the accommodation chambers, the battery cells are electrically connected to the common electrodes. The heatsink device is then coupled to the primary retaining frame and / or the secondary retaining frame, such that the heatsink device abuts against the top common electrode and / or the bottom common electrode of the battery cells.

An electric circuit for equalizing voltages between serially arranged cells. The circuit is operable to generate AC voltages at amplitudes dependent on the DC voltages of the received cells. The AC voltages are all connected to an inductive coupling circuit. The inductive coupling allows higher-voltage cells to induce a recharging current flow in lower voltage cells and so equalize the cell voltages. The circuit also operates to ensure that cell voltages are maintained in equilibrium during charging or discharging of received cells, irrespective of the individual charging and discharging characteristics of the cells. The circuit thus prevents individual cells in a battery from being over-charged or over-discharged and allows more efficient use of the full capacity of a battery without risking damage to the individual cells. The cell equalizing circuit is thus especially useful for rechargeable cells, such as lithium-ion cells, which are sensitive to damage by over-charging and over-discharging.

A battery cell charging system, including a charger and a controller, for rapidly charging a lithium ion battery cell, the battery cell charging system having a circuit for charging the battery cell using an adjustable voltage charging-profile to apply a charging voltage and a charging current to the battery cell wherein the adjustable voltage charging-profile includes: a first charging stage with a constant first stage charging current and an increasing battery cell voltage with the first stage charging current provided until the first stage charging voltage is about equal to a first stage complete voltage less than a maximum battery cell voltage; an intermediate ramped charging stage, the intermediate ramped charging stage including both an increasing ramped voltage and a decreasing ramped iBat current for the battery cell for the voltage charging range of the first stage complete voltage to about the maximum battery cell voltage; and a final charging stage with a constant final stage charging voltage about equal to the maximum battery cell voltage and a decreasing final stage charging current with the final stage charging voltage provided until the final stage charging current reaches a desired charge complete level.

Compositions and methods for enhancing repair of damaged DNA in skin cells. Topical compositions comprising at least one agent that upregulates circadian gene expression in skin cells and at least one non-circadian agent that delays mitosis in skin cells. Preferably, such compositions comprise one or more keratinocyte clock and per1 gene activators, along with SIRT1 or one or more sirt1 activators. More preferably, the sirt1 activator is a synergistic combination of specific peptidic sirt1 activators and resveratrol. Preferably, such compositions are easy to use, efficacious, cosmetically acceptable, chemically, thermodynamically and light stable, safe for topical use, have little or no side effects, and commercially feasible in a personal care marketplace.

A battery cell charger for rapidly charging a lithium ion battery cell (or string of series-parallel connected cells) having a maximum battery cell voltage the battery cell charging system including: a circuit for charging the battery cell using an adjustable voltage charging-profile to apply a charging voltage and a charging current to the battery cell wherein the adjustable voltage charging-profile includes: a first charging stage with a constant first stage charging current and an increasing battery cell voltage with the first stage charging current provided until the first stage charging voltage is about equal to a first stage complete voltage less than the maximum battery cell voltage; one or more intermediate charging stages, each intermediate stage selected from the group consisting of one or more of an intermediate constant voltage stage that provides a decreasing charging current, an intermediate constant current stage that produces an increasing battery cell voltage, and combinations thereof; and a final charging stage with a constant final stage charging voltage about equal to an intermediate stage complete voltage and a decreasing final stage charging current with the final stage charging voltage provided until the final stage charging current reaches a desired charge complete level.

A battery cell charging system, including a charger and a controller, for low-temperature (below about zero degrees Celsius) charging a lithium ion battery cell, the battery cell charging system includes: a circuit for charging the battery cell using an adjustable voltage charging-profile to apply a charging voltage and a charging current to the battery cell wherein the adjustable voltage charging-profile having: a non-low-temperature charging stage for charging the battery cell using a charging profile adapted for battery cell temperatures above about zero degrees Celsius; and a low-temperature charging stage with a variable low-temperature stage charging current that decreases responsive to a battery cell temperature falling below zero degrees Celsius.

A cell culture microcarrier includes a polymer formed from copolymerization of a mixture including (i) an uncharged hydrophilic unsaturated monomer having a hydroxyl group; (ii) a hydrophilic carboxylic acid containing unsaturated monomer; and (iii) a hydrophilic multifunctional unsaturated monomer. The microcarrier may further include a polypeptide, such as a polypeptide that promotes cell adhesion, conjugated to the surface of the microcarrier; e.g. via the carboxyl group from the hydrophilic carboxylic acid containing unsaturated monomer. Some of the microcarriers support attachment of human embryonic stem cells.

A battery cell charging system, including a charger and a controller, for rapidly charging a lithium ion battery cell, the battery cell charging system having a circuit for charging the battery cell using an adjustable voltage charging-profile to apply a charging voltage and a charging current to the battery cell wherein the adjustable voltage charging-profile includes: a first charging stage with a constant first stage charging current and an increasing battery cell voltage with the first stage charging current provided until the first stage charging voltage is about equal to a first stage complete voltage less than a maximum battery cell voltage; an intermediate ramped charging stage, the intermediate ramped charging stage including both an increasing ramped voltage and a decreasing ramped iBat current for the battery cell for the voltage charging range of the first stage complete voltage to about the maximum battery cell voltage; and a final charging stage with a constant final stage charging voltage about equal to the maximum battery cell voltage and a decreasing final stage charging current with the final stage charging voltage provided until the final stage charging current reaches a desired charge complete level.

A battery cell charger for rapidly charging a lithium ion battery cell (or string of series-parallel connected cells) having a maximum battery cell voltage the battery cell charging system including: a circuit for charging the battery cell using an adjustable voltage charging-profile to apply a charging voltage and a charging current to the battery cell wherein the adjustable voltage charging-profile includes: a first charging stage with a constant first stage charging current and an increasing battery cell voltage with the first stage charging current provided until the first stage charging voltage is about equal to a first stage complete voltage less than the maximum battery cell voltage; one or more intermediate charging stages, each intermediate stage selected from the group consisting of one or more of an intermediate constant voltage stage that provides a decreasing charging current, an intermediate constant current stage that produces an increasing battery cell voltage, and combinations thereof; and a final charging stage with a constant final stage charging voltage about equal to an intermediate stage complete voltage and a decreasing final stage charging current with the final stage charging voltage provided until the final stage charging current reaches a desired charge complete level.

A novel HIV vaccine is provided. In particular, the vaccine comprises an avirulent and non-cytolytic recombinant HIV wherein the NSS of the virus' envelope glycoprotein is replaced with a non-cytolytic signal sequence and nef gene of the virus is deleted which renders the virus avirulent.

The present invention relates to the use of a mitochondrially targeted antioxidant, e.g. derivatives of vitamin E, coenzyme Q10 or a glutathione peroxidase mimetic, in the treatment and prevention of liver diseases and / or epithelial cancers. The present invention also relates to pharmaceutical compositions containing the antioxidant(s) intended for such use. Furthermore the invention relates to the manufacture of medicaments containing the antioxidant(s) useful for such prevention and treatment.

The present invention relates to the method of treatment or prophylaxis of patients with drug-induced liver diseases which comprises administering to the patient in need thereof a therapeutically effective amount of a mitochondrially targeted antioxidant compound e.g. derivatives of vitamin E, coenzyme Q10 or a glutathione peroxidase mimetic. The present invention also relates to pharmaceutical compositions containing the antioxidant(s) intended for such use.

The invention discloses essence for a facial mask for moistureizing, replenishing water and repairing skin and a preparation method of the essence. The essence for the facial mask comprises sodium hyaluronate, a soluble protein polysaccharide solution and a saccharide isomer, can act on skin and cuticle to form a water retention protection screen, and besides, can permeate into a derma layer to promote the synthesis of sodium hyaluronate, so as to achieve double effects of moisturizing and replenishing water. The essence for the facial mask also contains natural plant extraction complex liquid, folic acid and soybean fermentation complex liquid, oat beta-glucan and ectoine. Water molecules around cells can be gathered, and a hydration housing having the effects of protecting, nourishing and stabilizing cells is formed outside a cell membrane so as to prevent cell injury. An active immunity system can be activated for reaction, collagen can be synthetized, the immunity of the cells is improved, the release quantity of mast cells and leucocyte histamine with inflammation, can be reduced; and therefore, the effects of protecting and repairing skin and muscle protection screen can be achieved. The production technology of the essence for the facial mask is simple, and large-scale industrialized production is easy to realize.

A method of treating a problem relating to tissue metabolism by administering to a patient in need a single dose of glutamine in a pharmaceutically acceptable carrier. Also provided is glutamine for the treatment of an injury relating to tissue metabolism and for preventing cardiac cell damage. A therapeutic composition for treating and preventing cellular metabolic injury, preventing cardiac cell damage, and increasing heat shock protein expression where in the therapeutic includes a single dose of glutamine in a pharmaceutically acceptable carrier is also provided. A method of increasing the expression of heat shock proteins by administering a single dose of glutamine in a pharmaceutically acceptable carrier to a location in need is further provided.

Disclosed are novel protective compositions for dermal papilla cells. In an embodiment the protective compositions of the present invention comprise 0.25% w / w or above of compositions comprising at least 10% w / w and above of 1-O-galloyl-β-D-glucose (β-glucogallin). In an embodiment, the said protective composition additionally comprises 50% to greater than 50% gallates including mucic acid 1,4-lactone 5-O-gallate, mucic acid 2-O-gallate, mucic acid 6-Methyl ester 2-O-gallate, mucic acid 1-Methyl ester 2-O-gallate and ellagic acid. In another embodiment the invention also encompasses synergistic protective compositions comprising the said protective compositions and 0.5% concentrate of liquid endosperm of Cocos nucifera, for dermal papilla directed towards helping the dermal papilla cells to form sufficient numbers and to retain a healthy morphology conducive for hair growth.

The present invention is directed, in part, to fluorescent metal sensors for detecting metal ions, and methods of making and using the same.

This disclosure provides a method to reduce cell damage caused by near UV light absorption of algal or cyanobacterial cultures. The algal or cyanobacterial cells are transformed to express one or more fluorescent proteins, that absorb the harmful UV or near UV wavelengths and emits wavelengths that are photosynthetically more active. The photosynthetic pigments of the transgenic algal or cyanobacterial cell culture will then absorb the photosynthetically active light emitted by the fluorescent proteins. Accordingly the harmful effects of the UV and near UV radiation are reduced and the photosynthetic activity of the algal or cyanobacterial cells is improved.

An encapsulant sheet for solar cell modules comprising a transparent soft resin film having a plurality of hemispherical concave parts of varying sizes formed on one or both surfaces of the transparent soft resin film, can disperse pressure generated in the process of combining the encapsulant sheet and a solar battery cell to prevent damage to the solar battery cell and suppress bubble generation by allowing air to escape easily.

The present invention is directed, in part, to fluorescein-based ligands for detection of metal ions, and methods of making and using the same.

The invention discloses a cell preservation solution and a use thereof. The preservation solution comprises hydroxyethylpiperazineethanesulfonic acid, sodium hydrogencarbonate, glucose, trehalose, sodium L ascorbate and one or more antioxidants (such as MnTBAP, MnTMPyP, L-reduced glutathione and N-acetyl-L-cysteine) in a solution containing an electrolyte and amino acids. The cell preservation solution provides nutrients and metabolic energy for cells, contains various pH buffers and radical scavengers, can retain cell activity and functions at 2-8 DEG C or normal temperature, facilitates cellpreservation and transport and solves the problem that the cells need long function recovery time after traditional transport.

A cell culture microcarrier includes a polymer formed from copolymerization of a mixture including (i) an uncharged hydrophilic unsaturated monomer having a hydroxyl group; (ii) a hydrophilic carboxylic acid containing unsaturated monomer; and (iii) a hydrophilic multifunctional unsaturated monomer. The microcarrier may further include a polypeptide, such as a polypeptide that promotes cell adhesion, conjugated to the surface of the microcarrier; e.g. via the carboxyl group from the hydrophilic carboxylic acid containing unsaturated monomer.

An improved anticoagulant is based on a higher level of citric acid than is usual (at least about 0.2% weight by volume). The higher citrate is combined with an amino acid as a counterion. The amino acid prevents cellular damage often caused by elevated citrate levels. The amino acid citrate mixture also serves to preserve platelet concentrates and platelet rich plasma during room incubation. Not only does the amino acid citrate combination enhance platelet integrity, it completely inhibits the growth of bacteria such as Staphylococcus epidermidis. Collecting blood of plasma into such higher levels of citrate prevents activation of blood proteins so that fractions made from the blood or plasma have superior characteristics.