813results about How to "The right amount" patented technology

An electrical current is created across an electrically conductive medium comprising a cell which may be part of a tissue of a living organism. A first electrical parameter which may be current, voltage, or electrical impedance is measured. A second electrical parameter which may be current, voltage or a combination of both is then adjusted and/or analyzed. Adjustments are carried out to facilitate analysis and/or obtain a desired degree of electroporation. Analysis is carried out to determine characteristics of the cell membrane and/or tissue.

The invention relates to oligonucleotides labeled with an energy transfer acceptor useful in conjunction with fluorescent nucleic acid stains. The resulting oligonucleotides are useful for decreasing background fluorescence during amplification assays and in ligation assays, and for detecting hybridization.

An intelligent database infrastructure wherein the management of all database components is performed by and within the database itself by integrating management of various components with a central management control. Each individual database component, as well as the central management control, is self-managing. A central management control module integrates and interacts with the various database components. The database is configured to automatically tune to varying workloads and configurations, correct or alert about bad conditions, and advise on ways to improve overall system performance.

The instant invention describes an analytical assay to accurately measure an analyte in the presence of an interfering substance.

Electroporation is performed in a controlled manner in either individual or multiple biological cells or biological tissue by monitoring the electrical impedance, defined herein as the ratio of current to voltage in the electroporation cell. The impedance detects the onset of electroporation in the biological cell(s), and this information is used to control the intensity and duration of the voltage to assure that electroporation has occurred without destroying the cell(s). This is applicable to electroporation in general. In addition, a particular method and apparatus are disclosed in which electroporation and/or mass transfer across a cell membrane are accomplished by securing a cell across an opening in a barrier between two chambers such that the cell closes the opening. The barrier is either electrically insulating, impermeable to the solute, or both, depending on whether pore formation, diffusive transport of the solute across the membrane, or both are sought. Electroporation is achieved by applying a voltage between the two chambers, and diffusive transport is achieved either by a difference in solute concentration between the liquids surrounding the cell and the cell interior or by a differential in concentration between the two chambers themselves. Electric current and diffusive transport are restricted to a flow path that passes through the opening.

A fluid delivery system capable of delivering a precise amount of fluid and monitor certain properties of the fluid so that the correct fluid is safely delivered to its intended destination. The system makes use of a flow sensor comprising a freestanding tube portion vibrated at a resonant frequency, wherein the resonant frequency corresponds to the density of the fluid flowing through the tube portion and the tube portion exhibits a degree of twist that varies with the mass flow rate of the fluid flowing therethrough. Movement of the tube portion is then sensed to produce a first output signal corresponding to the fluid density and a second output signal corresponding to the mass flow rate. The system is also equipped to measure elapsed time and to stop fluid flow in response to either of the first and second output signals.

Disclosed is a medical infusion device including a dedicated processing unit for detecting abnormal operation of the operation of the device. Specifically, a watchdog controller is employed as an independent monitor of the functioning of microprocessors used in the medical infusion device for controlling things such as RF communication, insulin infusion, and system integrity. Through the use of an independently-powered watchdog control system, the accuracy and reliability of the device is enhanced, resulting in greater assurance to the patient receiving periodic or continuous infusion of a drug.

A user interface system for providing insurance including: a media capture unit including a camera; a controller in communication with the media capture unit; and a memory in communication with the controller; wherein the memory includes first asset information previously captured by an asset verification process in which a user captures first visual media information including at least one visual media information including an asset shown from a perspective, wherein the memory additionally including an asset verification software application that, when executed by the controller, causes the controller to: in response to receiving a notification of a loss regarding the asset, prompt the user to capture second asset information using a media prompt including directions for capturing at least one image including a view of the asset from the perspective; and transmit the second asset information to an underwriting server.

A control system for continuous mining machine to control the mining horizons of roof and floor horizons relative to the rock boundaries of an underground coal seam or ore vein. The mining machine is provided with a cutting drum mounted on a pivoted arm to allow for vertical displacement of the cutter to control the mining height. A plurality of sensors is carried on the rotatable arm to locate the roof and floor boundaries. Attitude sensors are mounted on the machine for providing signals indicative of the inclinations of the body of the machine, and, a microprocessor receives the signals from the boundary sensors and the attitude sensors for calculating the required roof cut and floor cut. A display device receives the signals from the microprocessor and displays the calculated results to the machine operator.

The new invention allows for easy retrieval of fall backs and eases the initial threading process upon initial opening/use of the package. Threading is done without removing the cap. Several of the embodiments allow refills to be inserted into the canister without removing the lid/cap. The layout of the dispensing system and the geometry and shape of the dispensing orifice/aperture minimize and mitigate product fall backs. The new invention improves performance of the orifices/apertures through unique geometry and shape as well as using different materials from existing products or the combination of multiple materials. Varying orifice diameter, co-molded density and stiffness or geometry of the actual lobes defining the dispensing aperture allows the precise amount of friction to be created in the dispensing opening for selectively grabbing or releasing the towelette, thereby tearing the towelette connecting perforations at just the right time.

A washing machine includes an electric motor generating a driving force for wash, rinse and dehydration operations, a current detector detecting electric current flowing into the motor, a torque control unit performing a vector control for the motor on the basis of the current detected by the current detector so that torque developed by the motor is rendered optimum for at least the wash and dehydration operations, a speed control unit controlling a rotational speed of the motor, based on the current detected by the current detector, and a laundry amount determining unit determining an amount of laundry in a rotating tub, based on a magnitude of torque current in a period when a rotational speed of the motor varies.

Methods for commercial production of transdermal formulations comprising a hormone compound are provided. In particular, methods for commercial scale production under an inert atmosphere of a transdermal formulation comprising a therapeutically effective amount of a hormone, preferably a testosterone compound, useful for the treatment of hypoactive sexual desire disorder (HSDD) in postmenopausal women are provided.

A method and apparatus is used to confirm the charge amount and degradation state of a battery. Various cycle test battery measurements are conducted at prescribed time intervals until the end of the battery life, and the measured values are used to generate a determination table or determination tables showing relationships between battery charge amount and degradation state. To establish the charge amount and degradation state of a subject battery, the subject battery is measured and the results compared with determination table values. The existing charge amount and the state of battery degradation are estimated in accordance with a determination table location of matching values.

A slotted pin tool, a delivery system containing the pin tool, a substrate for use in the system and methods using the pin tool and system are provided. The slotted pin tool contains a plurality of pins having slotted ends designed to fit around each loci of material deposited on a surface, such as a microarray, without contacting any of the deposited material. Sample is delivered by contacting the pin tool with the surface; the amount delivered is proportional to the velocity of the pin tool as it contacts the surface or the velocity of the liquid when movement of the pin is halted.

A image sticking compensating device according to example embodiments includes a degradation calculator configured to calculate a degradation weight based on input image data, and to calculate degradation data of a frame, an accumulator configured to accumulate the degradation data, and to generate age data using the accumulated degradation data, and a compensator configured to determine a grayscale compensation value corresponding to the age data and an input grayscale of the input image data, and to output age compensation data by applying the grayscale compensation value to the input image data.