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Antioxidative animal bifidobacterium and applications thereof

A technology of animal bifidobacteria and live bacteria, applied in the direction of application, medical preparations containing active ingredients, bacteria, etc., to achieve the effect of reducing levels, enhancing immunity, and improving antioxidant capacity

Active Publication Date: 2013-09-11
QINGDAO VLAND BIOTECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no antioxidant Bifidobacterium animalis with strong hydrogen peroxide resistance and good free radical scavenging effect.

Method used

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  • Antioxidative animal bifidobacterium and applications thereof
  • Antioxidative animal bifidobacterium and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Screening and Identification of Animal Bifidobacteria

[0021] Collect the middle and rear part of the fresh feces of healthy infants who are breastfed, smash the feces with a sterile glass rod, add 10 times the weight of PBS buffer, shake vigorously for 30 minutes, centrifuge at 2000g for 5min, collect the supernatant; centrifuge the supernatant at 5000g for 10min , collect the precipitate; wash the precipitate twice with PBS buffer; add a small amount of PBS buffer to fully suspend the precipitate; spread the suspension on BBL selective medium, anaerobic culture at 37°C for 48 hours; Strains were further streaked and purified.

[0022] One isolated strain was named YJM-Bi01. The strain is a polymorphic bacillus, which is Y-shaped, V-shaped, curved, and spatula-shaped. Its typical morphological feature is a bacillus with forks; the colony is smooth, convex, and the edges are complete. The cream is white, shiny and soft. texture. The bacterium was prelimina...

Embodiment 2

[0026] Example 2 Experiment of acid resistance and bile salt resistance of animal Bifidobacterium YJM-Bi01

[0027] 2.1 Acid resistance test

[0028] Adjust the pH value of the MRS liquid medium to 2.0, 3.0, and 4.0 respectively; sterilize at 121°C for 15 minutes; inoculate the activated Bifidobacterium animalis into the cooled medium according to the 2% inoculum amount, and culture at a constant temperature of 37°C Cultivate in the box, take samples at 0h, 1h, and 2h respectively, and count the plate to determine the number of viable bacteria. The results are shown in Table 1.

[0029] Table 1: Acid resistance test of animal bifidobacteria

[0030]

[0031] As can be seen from Table 1, after Bifidobacterium animalis of the present invention is cultivated in the MRS medium of pH2.0 for 1h, the live bacteria rate is as high as 86.75%, thereby illustrating that this bacterial strain has stronger acid tolerance, can effectively resist gastric juice .

[0032] 2.2 Bile salt ...

Embodiment 3

[0040] Example 3 In vitro antioxidant experiment of animal Bifidobacterium YJM-Bi01

[0041] Inoculate Bifidobacterium animalis into MRS liquid medium after activated culture, and culture at 37°C for 24 hours; centrifuge the culture solution at 4000r / min for 10 minutes, and separate the supernatant, which is the cell-free extract; Wash twice, then resuspend the bacteria, and adjust the bacterial concentration to about 10 9 cfu / mL; the resulting bacterial suspension was divided into two groups, one group was used as living cells, and the other group was ultrasonically crushed for 10 minutes, no complete bacterial cells were detected under a microscope, then centrifuged at 4°C, 6000r / min for 10 minutes, and the supernatant was collected , which is the cell lysate.

[0042] 3.1 Determination of the ability of animal bifidobacteria to scavenge DPPH free radicals

[0043]Take 2 mL each of the above-mentioned cell-free extract, cell lysate and viable cells, add 1 mL each of 0.2 mm...

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Abstract

The invention provides an antioxidative animal bifidobacterium strain with the preservation number is CCTCC (China Center For Type Culture Collection) NO:M2013282. The animal bifidobacterium can be used for preparing pharmaceutics, food or health-care products. With the animal bifidobacterium, the enzymatic activity of superoxide dismutase, glutathione peroxidase and catalase in an organism can be obviously improved, the level of malondialdehyde in the organism can be lowered, so that the antioxidation capability of the organism can be effectively improved, the immunity of the organism can be enhanced, and the antioxidative animal bifidobacterium can be widely applied in pharmaceutics, food or health-care products.

Description

technical field [0001] The invention relates to the technical field of microorganism application, in particular to an anti-oxidation animal bifidobacterium and its application. Background technique [0002] Free radicals are normal metabolites of the human body. Under normal circumstances, free radicals in the human body are in a dynamic balance of continuous generation and removal. A small amount of free radicals not only does not pose a threat to the human body, but also can promote cell proliferation, stimulate white blood cells and phagocytes to kill bacteria, eliminate inflammation, and decompose toxins; but if the number of free radicals in the human body is too large, it will damage the cell structure and cause Lipid peroxidation interferes with the normal metabolic activities of the human body, causes diseases, and accelerates the aging process of the human body. [0003] Free radicals are almost related to most common human diseases, such as cardiovascular disease,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P39/06A61P37/04A23L1/29C12R1/01A23L33/00A61K35/745
Inventor 王浩彭虹旎朱彩彩陈琳卫镜明
Owner QINGDAO VLAND BIOTECH GRP
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