The invention discloses a kit for determining haptoglobin and a preparation method thereof. The kit comprises dual-liquid components of a reagent R1 and a reagent R2 which are independent from each other. The reagent R1 is prepared from a buffer solution, an accelerator, a surface active agent, inorganic salt ions and a corrosion remover, and a solvent of the reagent R1 is purified water; the reagent R2 is prepared from a buffer solution, a suspending agent, a surface active agent, inorganic salt ions, a corrosion remover and haptoglobin antibodies, and a solvent of the reagent R2 is purified water. The preparation method comprises the steps that the reagents are prepared according to the component content; a to-be-tested sample is mixed with the reagent R1 and the reagent R2 to be subjected to a sufficient reaction; a fully automatic biochemical analyzer is used for determining an absorptivity difference obtained after the reaction; according to an absorptivity change value, the concentration of haptoglobin in the sample is worked out. The kit has the advantages of being convenient to operate, high in accuracy and the like.