56 results about "C region" patented technology

A reshaped human antibody to the human IL-6R, comprising: (A) an L chain comprising, (1) a human L chain C region, and (2) an L chain V region comprising human L chain framework regions (FRs), and mouse L chain complementary determination regions (CDRs) of a momoclonal antibody to the IL-6 receptor (IL-6R); and (B) an H chain comprising, (1) a human H chain C region, and (2) an H chain V region comprising human H chain FRS, and mouse H chain CDRs of a monoclonal antibody to the IL-6R. Since major portion of the reshaped human antibody is derived from a human antibody and the mouse CDRs which are less immunogenic, the present reshaped human antibody is less immunogenic to human, and therefor is promised for therapeutic uses.

A reshaped antibody comprising: (A) L chains comprising: (1) a human C region, and (2) an L chain V region comprising human L chain FRs and L chain CDRs of a mouse monoclonal antibody; and (B) H chains comprising: (1) a human H chain C region, and (2) an H chain V region comprising human H chain FRs, and H chain CDRs of a mouse monoclonal antibody to human IL-6. Since the major portions of the reshaped human antibody are derived from human, and the mouse CDRs are less immunogenic, then the present reshaped human antibody is less immunogenic, and therefore inhibits information transfer by IL-6, and is promising as a therapeutic agent for diseases caused by IL-6.

An objective of the present invention is to provide a zinc oxide (ZnO) single crystal whose electroconductivity is excellent and which has a high quality. The invention relates to a zinc oxide single crystal whose concentration of metals other than zinc in the crystal fulfills the following equation:[−cM] / [+cM]≧3wherein M is a metal other than zinc, [−cM] is a concentration of M in a −c region in the zinc oxide crystal, and [+cM] is a concentration of M in a +c region in the zinc oxide crystal.

The invention provides a method for carrying out high-throughput sequencing on a TCR (T cell receptor) or a BCR (B cell receptor). The method is characterized by designing upstream primers according to gene features of a V region of the TCR or the BCR and designing downstream primers according to gene features of a C region or a J region of the TCR or the BCR and obtaining sequences of the of the TCR or the BCR in combination with the multiplex PCR (polymerase chain reaction) technology and high-throughput sequencing, thus analyzing the rearrangement information of the TCR or the BCR. Compared with 25-30 cycles of existing multiplex PCR, two cycles of the multiplex PCR technology provided by the invention can conduce to greatly reducing the sequencing errors caused by primer amplification preference. Besides, the invention also provides a method for correcting multiplex PCR (polymerase chain reaction) primer deviation by utilizing DNA (deoxyribonucleic acid) tag sequences, thus further reducing the sequencing errors caused by primer amplification preference and intrinsic sequencing errors of high-throughput sequencing.

The present invention provides a method for constructing a variable region sequencing library, and a method for determining a variable region nucleic acid sequence. The method for constructing the variable region sequencing library comprises: adopting a sample containing nucleic acid encoding the variable region as a template, amplifying the nucleic acid encoding the variable region so as to obtain a first amplification product, and adding a sequencing linker to at least one of both terminals of the first amplification product so as to obtain the variable region sequencing library, wherein the primers used by the amplifying comprise: a first primer comprising at least a nucleic acid molecule specifically recognizing the framework encoding sequence of the V region, and a second primer comprising at least a nucleic acid molecule specifically recognizing the encoding sequence of the J region or C region. With the method of the present invention, the variable region sequencing library can be effectively constructed.

The invention relates to a method of diagnosis of Huntington's Disease in a diagnostic sample of a valid body tissue taken from a human subject, which comprises detecting an altered concentration of a protein in the diagnostic sample, compared with a sample of a control human subject, the protein being selected from: Swiss Prot accession number: Protein name; P10909: Clusterin precursor; P00738: Haptoglobin precursor; P01009: Alpha-1-antitrypsin precursor; P01024: Complement C3 precursor; P01620: 1 g kappa chain V-III region; P01834: 1 g kappa chain C region P01842: 1 g lambda chain C regions; P01857: 1 g gamma-1 chain C region; P01859: Ig gamma-2 chain C region; P01876: 1 g alpha-1 chain C region P02647: Apolipoprotein A-I precursor; P02649: Apolipoprotein E precursor; P02652: Apolipoprotein A-II precursor; P02655: Apolipoprotein C-II precursor; P02656: Apolipoprotein C-II precursor P02671: Fibrinogen alpha / alpha-E chain precursor; P02763: Alpha-1-acid glycoprotein 1 precursor; P02766: Transthyretin precursor; P02768: Serum albumin precursor; P02787: Serotransferrin precursor; P04196: Histidine-rich glycoprotein precursor; P06727: Apolipoprotein A-IV precursor; P19652: Alpha-1-acid glycoprotein 2 precursor; P68871 / P02042: Hemoglobin beta chain / Hemoglobin delta chain; P60709: Beta actin.

The invention relates to the field of image processing. The objectives of the invention are to avoid the condition that superposed regions are irregular graphs after splicing and eliminate slits after the splicing of images more quickly and precisely. The technical scheme is that the rapid image fusing method for eliminating splicing slits comprises steps of: Step1: firstly setting a threshold value T, wherein the range of the threshold value is 16-64 pixel points; Step 2: searching regions in the superposed regions, wherein the distance between the regions and the splicing slits is not greater than the threshold value in the positive direction of the x axis and the negative direction of the y axis, the distance between the A region and the upper boundary slit is not greater than the threshold value, the distance between the B region and the right boundary slit is not greater than the threshold value and the distance between the C region and the upper boundary slit and the right boundary slit is not greater than the threshold value; and Step 3: carrying out weighted average operation on points in transition regions. The rapid image fusing method is mainly used in design and manufacturing occasions.

The present invention discloses a specific recognition sequence based T cell receptor high-throughput sequencing library construction and sequencing data analysis method. According to the method, a specific reverse transcription primer is designed for an mRNA sequence of a C region of a TCR constant region, cDNA is obtained by reverse transcription, and the 3' end of the cDNA is connected with a library construction linker with a specific recognition sequence; then the linker with the specific recognition sequence is added by using a splint connection method, and a TCR gene rearrangement sequence is amplified under the action of DNA polymerase by utilizing a gene specific primer with a tag; and finally a DNA library is added with a sequencing linker by PCR amplification to prepare a high-throughput sequencing library, and the high-throughput sequencing library is used for sequencing. The TCR gene diversity is comprehensively analyzed by bioinformatics, and a rearrangement rule of TCR genes including J region, D region and V region genes can be accurately obtained. The method is high in library construction efficiency, few in library construction step, low in required RNA initial quantity and low in library construction cost.

The present application relates to urinary protein markers for chronic pancreatitis and uses thereof. Specifically, this application relates to the use of urine protein markers obtained by using chronic pancreatitis disease models and mass spectrometry analysis in the early diagnosis of human chronic pancreatitis disease and the use of disease course monitoring. The urine protein markers are selected from Igγ‑1 chain C domain, nucleonectin‑2, beta‑2‑microglobulin, interleukin‑4 receptor alpha subunit, phosphoglycerate mutase 1, dipeptidyl peptidase 2, secreted pyrophosphoprotein 24, glycans Core protein, endothelial cell selective adhesion molecule, nestin-2, serum amyloid substance p, nerve cell adhesion molecule 1, etc. In this application, the obtained differential protein in urine provides a non-invasive and convenient option for early diagnosis and subsequent treatment of chronic pancreatitis.

The invention relates to a protein marker of focal segmental glomerulosclerosis, and more specifically relates to an application of a connecting part selectively connected to one or more proteins in preparing a diagnostic reagent in a development process of human focal segmental glomerulosclerosis. The one or more proteins are selected from [alpha]1 antiprotease, alpha albumin, serum albumin, beta-2-microglobulin, ceruloplasmin, fetuin-B, kininogen, serum transferrin, carboxylesterase, immune globulin gamma-2A chain C region, plasminogen, lysosomal acid phosphatase, protein YIPF3, zinc [alpha]2 glycoprotein, alpha-2-HS-glycoprotein, protein AMBP or T cell immune globulin. Specifically, the invention relates to the application of a urine protein potential marker of human focal segmental glomerulosclerosis obtained by a rat model and mass spectrometry.

The invention relates to a method and a kit for detecting mutations in the pre-C region and core promoter region (BCP) of the hepatitis B virus (HBV) genome in clinical blood samples, in particular to rapid detection of the pre-C / BCP region of the HBV gene by reverse dot hybridization technology The method of mutation and the kit used.

A structure and method where C is incorporated into the collector region of a heterojunction bipolar device by a method which does not include C ion implantation are provided. In the present invention, C is incorporated into the collector by epitaxy in a perimeter trench etched into the collector region to better control the carbon profile and location. The trench is formed by etching the collector region using the trench isolation regions and a patterned layer over the center part of the collector as masks. Then, Si:C is grown using selective epitaxy inside the trench to form a Si:C region with sharp and well-defined edges. The depth, width and C content can be optimized to control and tailor the collector implant diffusion and to reduce the perimeter component of parasitic CCB.

A releasable resin composition which has excellent thermal stability during extrusion molding, etc., does not cause problems such as fuming and foaming during compound preparation or extrusion molding, and does not foul metallic rolls. It can form a release layer which, when contacted with a pressure-sensitive adhesive layer, is free from a problem that an impurity migrates from the release layer to reduce pressure-sensitive adhesive performance. The releasable resin composition comprises a release agent (A) comprising as the main component a polymer having a C8-30 aliphatic group and having a weight-average molecular weight of 10,000-1,000,000 and a thermoplastic polymer (B) comprising constituent units derived from an olefin monomer and / or a polar monomer, the content of the release agent (A) being 0.1-20 parts by weight per 100 parts by weight of the thermoplastic polymer (B). The release agent (A) has a 2 percent weight loss temperature as determined by thermogravimetric analysis in a 260-275 DEG C region, excluding 275 DEG C, and a 5 percent weight loss temperature in a 275-330 DEG C region, excluding 330 DEG C.

The invention relates to scandium-doped zinc oxide (Sc:ZnO) crystals and a preparation method thereof. In the invention, a small amount of scandium (Sc) is added in the preparation of ZnO to effectively improve the growing speed of an m plane and make quick growing of large-size ZnO crystals possible; the shapes of the crystals are reasonably changed to improve the growing efficiency of the crystals; usually, the ZnO crystals grow in hexagonal pyramid shapes, but after being doped with the scandium, the crystals grow in hexagonal prism shapes while keeping the +c slice area available above a +c region constant; and after scandium doping, the phenomenon that twin crystals are likely to develop in the growing process of the ZnO crystals and other phenomena are improved. Compared with the prior art, the grown Zinc oxide single crystals have the characteristics of high growing speed (particularly on the m plane), high growing efficiency, high crystal quality and the like. The method has high repeatability and low cost and is applicable to batch production.

The invention relates to a rapid dynamic power control method for reducing WLAN interference, and the method comprises the following steps: setting a group of sending rates of V1, V2 and V3 in a wireless device in a WLAN network, wherein the size of the sending rates is as follows: V1 is more than V2 and more than V3; setting a group of sending success rates of P1, P2 and P3 in the wireless device in the WLAN network, wherein the size of the sending success rates is as follows: P1 is more than P2 and more than P3; using the sending rates and the sending success rates to divide power into fourregions of A, B, C and D, wherein the A region is the power slow reduction region, the B region is the power retention region, the C region is the power rapid increase region, and the D region is themaximum sending power region; and collecting the current data sending rate, comparing with the before sending success rate, and determining sending power. The rapid dynamic power control method has the advantages that the rapid dynamic power control method can reduce the interference of the device while retaining the stable high bandwidth, and not affect the coverage and the anti-interference performance of the device.

The invention discloses a dual-core controller online upgrading system of DSP and ARM based on WIFI and a method thereof. The system comprises servers, wireless routers and several devices; the deviceincludes ARM, DSP and a WIFI module. The device establishes wireless connection with a wireless router through the WIFI module, the wireless router and the server establish communication link, and ARM connects with DSP through an SPI bus. The internal FLASH of ARM has four regions: A, B, C and D. The A region is used to store the BootLoader of ARM, the B region and the C region are used to storethe user program, the D region is used to store the related parameters. DSP includes internal FLASH, internal RAM, and external SRAM. The invention has the following beneficial effects: 1, the invention can upgrade the DSP and the ARM of the controller at the same time; 2, the peripheral circuit of the upgrade system in the invention is simple, and the remote upgrade can be realized without complex circuit; 3, the software of the host compute in the invention supports upgrade DSP or ARM program separately; 4, the upper computer software of the invention supports the simultaneous batch online upgrade program.

The invention belongs to the field of molecular biological detection, and in particular relates to an RNA linker and a single pair of primers for constructing a high-throughput sequencing library for a T cell receptor (TCR) of T cell leukemia minimal residual disease based on high-throughput sequencing, and a preparation method of the DNA library. The method comprises the following steps: acquiring 10mL of a human blood sample, and placing the human blood sample in an EDTA anticoagulative tube; separating peripheral blood mononuclear cells (PBMC) by adopting lymphocyte separating liquid Ficoll-1077; extracting total RNA of PBMC by adopting a Trizol method, wherein the used reagent is RNAzol RT; carrying out inverse transcription on RNA to form cDNA, and meanwhile, adding a linker at the 5' terminal of cDNA; carrying out PCR1; carrying out PCR2 and purification; and carrying out high-throughput sequencing, and thus the full-length information of a TCR gene sequence is obtained from an upstream primer of the linker and a downstream primer of the C region.

The invention provides a kit for detecting the drug-resistant mutation in the YMDD motif region of hepatitis B virus, and belongs to the technical field of molecular biology. Specifically, we designedspecific primers and probes for rtM204I / V (C region) + / - rtL180M (B region) mutation of HBV YMDD resistance gene by using RNase H2 and Taq DNA polymerase biphasic enzyme system to detect drug resistance mutation of HBV YMDD motif region. The kit provided by the invention has the advantages of high sensitivity, fast detection, good stability and closed detection, and reduces the possibility of later pollution.

The invention discloses a green sterilization and color protecting method for fresh-cut fruits and vegetables. The method comprises the following steps: S1, cutting the fruits and vegetables, puttingthe cut fruits and vegetables into micro-acid electrolyzed water, and soaking the cut fruits and vegetables under ultrasonic waves; and S2, irradiating the ultrasonically treated fruits and vegetablesby using ultraviolet light in a UV-C region. The green sterilization and color protection method for the fresh-cut fruits and vegetables ultrasonically soaks the fruits and vegetables in the micro-acid electrolyzed water, and carries out ultraviolet irradiation, so that the propagation of harmful microorganisms can be effectively inhibited; the method has strong sterilization capability, and hasbetter effects of inhibiting bacterial growth, preventing browning of the fruits and vegetables and improving the storage quality compared with single water ultrasonic soaking, ultraviolet irradiationand single micro-acid electrolyzed water ultrasonic soaking.

An assay for testing a subject for diabetes or a predisposition to diabetes including analyzing a biological fluid from a subject for the presence of one or more proteins selected from the group consisting of Alpha 2 macroglobulin, Apolipoprotein AII, Immunoglobulin alpha heavy chain constant region, Immunoglobulin mu chain C region, Chain A of Human IgA1, Inter-alpha-trypsin inhibitor heavy chain H4 precursor, and Apolipoprotein B11; wherein detection of the protein is indicative of diabetes or a predisposition to diabetes in the subject.

An assay for testing a subject for diabetes or a predisposition to diabetes including analysing a biological fluid from a subject for the presence of one or more proteins selected from the group consisting of Alpha 2 macroglobulin, Apolipoprotein All, Immunoglobulin alpha heavy chain constant region, Immunoglobulin mu chain C region, Chain A of Human IgA1, Inter-alpha-trypsin inhibitor heavy chain H4 precursor, and Apolipoprotein B100; wherein detection of the protein is indicative of diabetes or a predisposition to diabetes in the subject

Protective high molecular weight (HMW) proteins are produced recombinantly by expression from E. coli by using a promoter effective in E. coli and a nucleic acid molecule which contains a modified operon of a non-typeable strain of Haemophilus. The modified operon contains the portion only of the A region which encodes the mature HMW protein and the complete B and C regions of the operon. Enhanced levels of expression of the HMW proteins can be achieved by including the E. coli cer gene, a further copy of the portion of the A region of the operon encoding the mature protein or both in the expression vector. Nucleotide and deduced amino acid sequences of the hmw1 and hmw2 genes and HMW1 and HMW2 proteins, respectively of several non-typeable Haemophilus influenzae strain have been identified.

The invention relates to a method of diagnosis of Huntington's Disease in a diagnostic sample of a valid body tissue taken from a human subject, which comprises detecting an altered concentration of a protein in the diagnostic sample, compared with a sample of a control human subject, the protein being selected from: Swiss Prot accession number: Protein name; P10909: Clusterin precursor; P00738: Haptoglobin precursor; P01009: Alpha-1-antitrypsin precursor; P01024: Complement C3 precursor; P01620: 1g kappa chain V-III region; P01834: 1 g kappa chain C region P01842: 1g lambda chain C regions; P01857: 1g gamma-1 chain C region; P01859: Ig gamma-2 chain C region; P01876: 1g alpha-1 chain C region P02647: Apolipoprotein A-I precursor; P02649: Apolipoprotein E precursor; P02652: Apolipoprotein A-II precursor; P02655: Apolipoprotein C-II precursor; P02656: Apolipoprotein C-II precursor P02671: Fibrinogen alpha / alpha-E chain precursor; P02763: Alpha-1-acid glycoprotein 1 precursor; P02766: Transthyretin precursor; P02768: Serum albumin precursor; P02787: Serotransferrin precursor; P04196: Histidine-rich glycoprotein precursor; P06727: Apolipoprotein A-IV precursor; P19652: Alpha-1-acid glycoprotein 2 precursor; P68871 / P02042: Hemoglobin beta chain / Hemoglobin delta chain; P60709: Beta actin.

The invention provides a method for carrying out high-throughput sequencing on a TCR (T cell receptor) or a BCR (B cell receptor). The method is characterized by designing upstream primers according to gene features of a V region of the TCR or the BCR and designing downstream primers according to gene features of a C region or a J region of the TCR or the BCR and obtaining sequences of the of the TCR or the BCR in combination with the multiplex PCR (polymerase chain reaction) technology and high-throughput sequencing, thus analyzing the rearrangement information of the TCR or the BCR. Compared with 25-30 cycles of existing multiplex PCR, two cycles of the multiplex PCR technology provided by the invention can conduce to greatly reducing the sequencing errors caused by primer amplification preference. Besides, the invention also provides a method for correcting multiplex PCR (polymerase chain reaction) primer deviation by utilizing DNA (deoxyribonucleic acid) tag sequences, thus further reducing the sequencing errors caused by primer amplification preference and intrinsic sequencing errors of high-throughput sequencing.

The present invention provides a breast cancer marker selected from the group consisting of fucosylated hornerin, fucosylated Zn-α-2-glycoprotein, fucosylated Ig γ-1 chain C region, and fucosylated desmoplakin; a method for determining breast cancer comprising detecting the breast cancer marker in a sample, and determining on the basis of the results of the results; and a kit to be used for the determination.

Disclosed are a chemical vapour deposition method for an organic metal compound and an apparatus therefor. The method comprises: providing a base and at least one substrate; providing a first gas inlet device and a second gas inlet device, the spraying direction of a first gas along a first gas outlet forming an included angle with the spraying direction of a second gas along a second gas outlet; depositing the first gas and the second gas on the surface of the substrate to obtain a layer of organic metal compound; the first gas being distributed in the reaction region in a concentration gradient, comprising an A region and a B region, with the average gas concentration in the A region being higher than that in the B region; the second gas being distributed in the reaction region in a concentration gradient, comprising a C region and a D region, with the average gas concentration in the C region being higher than that in the D region; the A regions and the C regions being arranged alternately; and the substrate passing through the A region and the C region in sequence. The present invention can not only avoid the premature reaction of the reactive gases, but also improve the reaction rate and reduce production costs.

The present invention addresses the problem of providing a combination of biomarkers, whereby integration dysfunction syndrome can be highly accurately diagnosed, and the utilization thereof. Provided is an integration dysfunction syndrome marker set which comprises two or more protein molecules combined together, said protein molecules being selected from the group consisting of trifunctional purine biosynthetic protein adenosine-3, uroporphyrinogen decarboxylase, interferon-induced GTP-binding protein Mx1, glutaredoxin-3, microtubule-associated protein RP / EB family member 1, tubulin folding cofactor B, immunoglobulin mu chain C region and heat shock 70 kDa protein 4L. Also provided is a method for examining integration dysfunction syndrome with the use of the level of the aforesaid marker set in a specimen as an index.

The invention relates to a dimmable Tai-Chi style dual-color COB, which includes a substrate, an LED chip fixed on the substrate, positive and negative soldering lugs electrically connected with the LED chip, and a phosphor layer applied to the LED chip. The dimmable Tai-Chi style dual-color COB is characterized in that the LED chip is arranged into a circular illumination area on the substrate, and the circular illumination area is surrounded by an annular dam; a curved dam is arranged in the illumination area to divide the illumination area into a Tai-Chi style; and the illumination area isdivided into two blocks which are a C region and a W region respectively, wherein the C region and the W region have different color temperatures, the area of the C region and the area of the W regionare the same, and the power of the LED chip in the C region is equal to that of the LED chip in the W region. The dark area of illumination can be eliminated. Color mixing can be more uniform. The lighting effect is better. A wider color range is achieved.