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Application of mulberry polysaccharide extract in preparing medicine or health product

A technology of mulberry polysaccharides and extracts, which is applied in the application field of medicine or health care products, and can solve the problems that there is no mulberry polysaccharide extract, no mulberry liver protection effect, etc.

Inactive Publication Date: 2015-12-16
GUIZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although it has been found that mulberry has the effect of protecting the liver, there is no in-depth research report on the effect of mulberry on protecting the liver in the prior art. Related reports on polysaccharide extracts used in chemical liver injury protection products

Method used

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  • Application of mulberry polysaccharide extract in preparing medicine or health product
  • Application of mulberry polysaccharide extract in preparing medicine or health product
  • Application of mulberry polysaccharide extract in preparing medicine or health product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1 Mulberry polysaccharide extract is prepared according to the following method steps:

[0071] (1) Impurity removal: wash 100g of mulberry raw material with water, dry, pulverize, and set aside;

[0072] (2) Degreasing and decolorization: Place the raw material obtained in step (1) in a container, first add 200mL75% ethanol to reflux twice, then add 200mL90% ether to reflux twice, each time to reflux for 60 minutes, after the reflux is completed, dry in the air, spare;

[0073] (3) Put the product obtained in step (2) in a container, add water 25 times the weight of the product obtained in step (2), adjust the pH value to 7, keep the temperature at 75 ° C, add 0.006 times the weight of the product obtained in step (2) Glucose oxidase is used for enzymolysis, and the enzymolysis time is 120 minutes;

[0074] (4) ultrasonically treating the enzymatic hydrolysis product in step (3), the ultrasonic frequency is 20KHz, and the time is 10 minutes to obtain an ex...

Embodiment 2

[0077] Embodiment 2 Mulberry polysaccharide extract is prepared according to the following method steps:

[0078] (1) Impurity removal: wash 150g of mulberry raw material with water, dry, pulverize, and set aside;

[0079] (2) Degreasing and decolorization: Place the raw material obtained in step (1) in a container, first add 200mL90% ethanol to reflux twice, then add 200mL95% ether to reflux twice, each time to reflux for 150 minutes, after the reflux is completed, dry in the air, spare;

[0080] (3) Put the product obtained in step (2) in a container, add water 6 times the weight of the product obtained in step (2), adjust the pH value to 3, keep the temperature at 25°C, add 0.001 times the weight of the product obtained in step (2) Glucose oxidase was used for enzymolysis, and the enzymolysis time was 15 minutes;

[0081] (4) ultrasonically treating the enzymatic hydrolysis product in step (3), the ultrasonic frequency is 100KHz, and the time is 40 minutes to obtain an ex...

Embodiment 3

[0084] Embodiment 3 Mulberry polysaccharide extract is prepared according to the following method steps:

[0085] (1) Impurity removal: wash 200g of mulberry raw material with water, dry, pulverize, and set aside;

[0086] (2) Degreasing and decolorization: Place the raw materials obtained in step (1) in a container, first add 400mL of absolute ethanol to reflux twice, then add 400mL of anhydrous ether to reflux twice, and reflux for 105 minutes each time. dry, spare;

[0087] (3) Put the product obtained in step (2) in a container, add water 11 times the weight of the product obtained in step (2), adjust the pH value to 5, keep the temperature at 58 ° C, add 0.003 times the weight of the product obtained in step (2) The glucose oxidase was used for enzymolysis, and the enzymolysis time was 38 minutes; the glucose oxidase was formulated into a 0.2wt%-0.4wt% aqueous solution for use;

[0088] (4) ultrasonically treating the enzymatic hydrolysis product in step (3), the ultras...

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Abstract

The invention discloses an application of a mulberry polysaccharide extract in preparing a medicine or a health product capable of preventing or inhibiting chemical liver injury. The mulberry polysaccharide extract has relatively wide biological activity and high activity, and can obviously reduce the levels of alanine aminotransferase and aspartate transaminase in serum, effectively improve the activities of superoxide dismutase and glutathion peroxidase in the liver, and obviously reduce the content of glutathione and malonaldehyde. The mulberry polysaccharide extract can be used for preparing a medicine or a health product, in particular for preparing the medicine or the health product having an inhibiting or preventing effect for the chemical liver injury.

Description

technical field [0001] The invention relates to a new application of a mulberry polysaccharide extract, in particular to the application in the preparation of medicines or health care products for preventing or inhibiting chemical liver damage, and belongs to the technical field of traditional Chinese medicine. Background technique [0002] Mulberry (Fructus Mori) has another name called mulberry jujube, mulberry fruit, mulberry fruit, black berry, etc. It has a wide range of cultivation and abundant resources. Fresh mulberry not only has a sweet taste, but also contains a lot of vitamins, amino acids, mineral elements, trace elements, inorganic salts, pectin, flavonoids, alkaloids, phenols and sugars and other nutrients, and has edible and medical properties. The role of health care, and thus loved by the majority of people. Mulberry is listed as one of the first batch of agricultural products "both food and medicine" by the Ministry of Health. Many famous works of tradi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P1/16A23L1/09
Inventor 周欣陈华国邓青芳赵超龚小见
Owner GUIZHOU NORMAL UNIVERSITY
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