174 results about "Aspartate Aminotransferases" patented technology

The invention provides a stable composition containing NAD+ or NADH. Bioactivity of NAD+ or NADH can be stably maintained for a long time. By the use of a stabilizer which contains trehalose, glycerol, a buffer solution and a surfactant, stability of NAD+ and NADH dissolved state is raised. The invention also provides a method for stabilizing NAD+ or NADH, and also provides an application of a composition composed of trehalose, glycerol, the buffer solution, the surfactant and a pH regulator in increasing stability of NAD+ or NADH. The composition can be used for a lactate dehydrogenase reagent, an alanine aminotransferase reagent, an aspartate aminotransferase reagent, a urea kid and the like, and is widely applied.

The present invention relates to a compound of Antrodia camphorata used for liver protection, in particular to an extract, 4-hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone which is isolated from Antrodia camphorata. The cyclohexenone compound according to the invention helps to alleviate liver injury and fibrosis induced by chemicals and reduces the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). By increasing the contents of glutathione peroxidase (GSHPx) and catalase (CAT), cyclohexenone further decreases the liver damage and the oxidative pressure caused by free radicals, enhances the antioxidant ability and achieves the purposed of liver protection.

Disclosed is a strain of Escherichia sp., capable of producing O-acetyl homoserine in high yield, with the introduction and enhancement therein of the activity of: homoserine acetyl transferase, aspartokinase and homoserine dehydrogenase; and at least one enzyme selected from a group consisting of phosphoenolpyruvate carboxylase, aspartate aminotransferase and aspartate semi-aldehyde dehydrogenase. Also, a method of producing O-acetyl homoserine using the strain is provided.

The invention discloses an interference-removing dry chemical quantitative test strip. The strip comprises a bottom liner, wherein the bottom liner is provided with a sample addition region and also a starting region; the sample addition region is fixed with interference-removing reagents and signal providing reagents; the starting region is fixed with test reaction starting reagents; and the sample addition region and the starting region are arranged on the test strip in a mode of maintaining a non-contact under a normal state and mutually contacting under a test state. The test method comprises the following steps of: adding a sample solution to be tested into the sample addition region, re-dissolving materials fixed on the sample addition region into the sample solution to be tested, and starting to perform a signal reaction to obtain a background signal value; and then enabling the starting region to contact with the sample addition region, re-dissolving materials fixed on the starting region, starting a test reaction, performing a second-time signal reaction on a product again, and estimating out a content of a biological enzyme to be tested according to a tested second-time characteristic value and the background signal value. The test method provided by the invention has the advantages of convenience for operation, low test cost, accurate test result and high test precision and can specifically be used in the alanine and aspartate aminotransferase quantitative test.

The invention relates to the technical field of medicaments and discloses the novel application of a penthorum chinense pursh extract or compounds of ursolic acid, pinocembrin, scopoletin, gallic acid, ursolic acid-28-xylose-(1-3)-glucoside, pinocembrin-7-glucoside, pinocembrin-7-neohesperidoside, scopolin or galloyl glucoside separated from the penthorum chinense pursh extract. Pharmacological and pharmacodynamic tests show that the penthorum chinense pursh extract or the compounds can obviously reduce the rising of aspartate aminotransferase (AST), glutamic-pyruvic transaminase (ALT) and alkaline phosphatase (ALP), caused by acute liver injury, alcoholic liver and virus hepatitis in mice, chronic hepatic fibrosis in rats and the like, has an obvious inhabitation effect on HepG2 tumor cells and can reduce the cholesterol and triglycercide content of blood serum. The penthorum chinense pursh extract or the compounds are simple in preparation method and low in cost. The invention provides a new medicament source for preventing and treating hepatitides, liver injury, hepatic fibrosis, cirrhosis, alcoholic liver, liver cancer and diseases associated with high blood fat.

Provided is a pharmaceutical or food or drink composition for preventing or treating alcohol-induced fatty liver or steatohepatitis comprising metadoxine (pyridoxol 1-2-pyrrolidone-5-carboxylate) and garlic oil as active ingredients. The concurrent administration of metadoxine and garlic oil according to the present invention exhibits outstandingly superior effects of inhibiting the accumulation of triglyceride and increase of blood AST (aspartate aminotransferase) level in liver el tissue, inhibiting the expression of FAS (fatty acid synthase), CYP2E1 and iNOS (inducible nitric oxide synthase) and inhibiting the deactivation of AMPK (AMP-activated protein kinase), as compared to the administration of metadoxine or garlic oil only.

The invention discloses a method for extracting coreopsis tinctoria procyanidins and an application of the coreopsis tinctoria procyanidins in delaying senescence. The method comprises the following steps: extracting coreopsis tinctoria procyanidins from the raw material coreopsis tinctoria which grows 2600m over the sea level by adopting a dynamic high-pressure microfluidization assisted extraction technology for 30 minutes with 70% ethanol at the extracting pressure of 120MPa and the extracting temperature of 50 DEG C, and treating the coreopsis tinctoria twice by means of microjet pressure. The total antioxidation activity of the prepared procyanidins is higher than that of ascorbic acid, and has an effect of delaying senescence of drosophila melanogaster by combining results of drosophila melanogaster surviving experiments and in-vivo biochemical index detection; liver injury experiments in mice indicate that the procyanidins extracted from coreopsis tinctoria is capable of reducing the activities of ALT (alanine aminotransferase) and AST (aspartate aminotransferase) in serum and playing a certain role in protecting CC14 caused liver injury in mice. According to the application, the procyanidins extracted from coreopsis tinctoria have an excellent effect of delaying senescence, and has a wide practical value.

The invention relates to the application of Ilex latifolia total glycosides and a preparation method thereof. Studies finds that the Ilex latifolia total glycosides can reduce the content of malonaldehyde (MDA) and triglyceride (TG) in liver, increase the content of reduced glutathione (GSH) in each dose group of liver, reduce the content of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in liver, and has alcoholic hepatitis and fatty liver resisting activity. Compared with the prior art, the invention provides a new application of Ilex latifolia total glycosides in preparing drugs and / or functional food for preventing and / or treating alcoholic hepatitis and fatty liver; the invention also provides a preparation method of the Ilex latifolia total glycosides with the advantages of safe and simple process, saved cost, high efficiency, and easy quality control; and the invention further provides a preparation of the Ilex latifolia total glycosides.

The invention discloses refined glossy privet fruit total glycosides capable of preventing and treating liver injury and a preparation method thereof. The preparation method is characterized in that the chemical compositions and pharmacological activity of the glossy privet fruit are researched and selected systematically; and the experiment results show that the selected refined glossy privet fruit total glycosides can obviously reduce serum ALT (Alanine Transaminase) and AST (Aspartate Aminotransferase) activities of a CCl4-induced liver injury model mice and the MDA (Methyl Di Aldehyde) content in the hepatic tissue of an alcohol-induced liver injury model mice and improve the GSH (Glutathione) activity of the liver, and also show that the refined glossy privet fruit total glycosides provided by the invention not only can obviously protect the liver from chemical liver injury and long-term drinking induced liver injury and can be developed into novel medicines or health products for preventing liver injury.

The invention belongs to the field of pharmacology of Chinese medicines and discloses an application of a Chinese herb Pien Tze Huang and a preparation thereof in preparation of drugs for treating non-alcoholic fatty liver disease (NAFLD). The Chinese herb Pien Tze Huang can be used for remarkably improving the liver function of a fatty liver rat, reducing the AST (Aspartate Aminotransferase), TG (Triglyceride) and (gamma-Glutamyl Transpeptidase) level of serum, reducing the degree of fatty liver and relieving inflammatory reaction and has a remarkable curative effect on the aspect of treating NAFLD.

The invention belongs to the field of molecular immunology, and in particular relates to an exosome secreted and released from the epidermal cells of a chicken liver, gall bladder and bile duct, and a preparation method of the exosome. The exosome contains hepatogenic proteins, such as C-reactive protein, aspartate aminotransferase, and vitronectin, epithelium-derived mucin and proteins related to an immune reaction, such as immunoglobulin, and can be taken as a biological indicator for studying liver diseases and as an adjuvant for immune reaction for immune-regulation, and can also be takenas a carrier for treating the diseases of livers, gall bladders and intestines.

The present invention relates to electrochemical detection technology, and is quick detecting method of aspartate aminotransferase vitality. Electrochemical or optical change is produced corresponding to the vitality of the measured matter. The method includes: A. sucking the measured matter liquid drop into the reaction area between electric poles to generate oxidation-reduction on the surface of the electric poles so as to produce electron transfer and form current; and B. converting the aspartic acid into glutamic acid and oxaloacetic acid under the cooperated action of current and auxiliary factors and measuring the amount of glutamic acid and oxaloacetic acid so as to obtain the corresponding aspartate aminotransferase vitality. The present invention has raised response speed and detection sensitivity of current type enzyme sensor and lowered reaction voltage.

The invention relates to an aspartate amino transferase (AST) detection kit and belongs to the technical field of clinic in vitro diagnostic reagents. The invention aims to provide the aspartate amino transferase detection kit with capability of effectively eliminating endogenous alpha-oxoglutarate interference and high stability in order to achieve more accurate and reliable determination results of aspartate amino transferase. The kit provided by the invention comprises a reagent 1 and a reagent 2, wherein the reagent 1 comprises the following components: Tris-HCl buffer solution, L-asparaginic acid, reduction coenzyme (NADH), malic dehydrogenase (MDH), enzyme protective agent, stabilizer and preservative, and the reagent 2 comprises the following components: Tris-HCl buffer solution, alpha-oxoglutarate, a stabilizer and a preservative. The enzyme protective agent and the stabilizer are added into the AST detection kit provided by the invention so that the stability of the aspartate amino transferase detection kit is promoted; the aspartate amino transferase detection kit is suitable for various full-automatic biochemical analyzers; the operation is simple and safe; the aspartate amino transferase detection kit has convenience in clinical application and popularization.

The invention discloses a co-culture system for liver cells and Kupffer cells and application thereof. The co-culture system is characterized in that in a Millicell double-layer culture room, 1-100 ng / mL LPS (lipopolysaccharides) is added into a culture medium, the Kuffer cells are cultured in the upper layer of the Millicell double-layer culture room, and the liver cells are cultured in the lower layer of the Millicell double-layer culture room; and half of the fresh culture medium is replaced every other day so as to keep the concentration of the LPS. The co-culture system for liver cells and Kupffer cells provided by the invention has the advantages that the Kupffer cells continuously secrete inflammatory cell factors (such as TNF-alpha) in vitro after being stimulated by the LPS; the liver cells can generate a great number of enzymes, such as AST (aspartate aminotransferase), ALT (alanine transaminase) and GGT (gamma-glutamyl transpeptidase) in the environment so as to stimulate the liver injury based on immunoreaction caused by the immune / inflammation response after the liver tissue undergoes virus infection in vivo; and moreover, the co-culture system is used for screening medicaments resisting immune liver injury according to the influence of the medicaments on the secretion amount of immune factors and the enzymatic activity.

The invention relates to traditional Chinese medicine composition with functions of relieving alcoholism and protecting the liver. The traditional Chinese medicine composition comprises raw medicinalmaterials as follows: flos puerariae, semen hoveniae, medicated leaven, fructus amomi rotundus, white poria cocos, radix ginseng, raw fructus crataegi, fructus schisandrae chinensis and raw oysters, and the raw medicinal materials are compatible and have the functions of eliminating dampness caused by alcohol and regulating qi-flowing for strengthening spleen. The pharmacological experiment indicates that with the adoption of the traditional Chinese medicine composition, drunkenness latency time of a mouse can be prolonged, drunkenness time of the mouse is shortened, content of alcohol in blood is reduced, level of ALT (alanine aminotransferase), AST (aspartate aminotransferase) and MDA (malondialdehyde) in the liver is reduced, activity of GSH-PX (glutathione peroxidase) and ADH (alcoholdehydrogenase) is improved, and the traditional Chinese medicine composition has excellent effects of relieving alcoholism and protecting the liver.

The invention relates to preparation of standards, and particularly relates to a recombinant human alanine aminotransferase protein standard, a recombinant human aspartate aminotransferase protein standard, and preparation methods thereof. A nucleotide sequence of encoding the recombinant human alanine aminotransferase protein standard is shown in SEQ ID NO:3; the nucleotide sequence of encoding the recombinant human aspartate aminotransferase protein standard is shown in SEQ ID NO:6. The invention also relates to the preparation method of the recombinant human alanine aminotransferase protein standard or the recombinant human aspartate aminotransferase protein standard. The preparation method comprises the following steps: obtaining a target gene; building a gene expression strain; expressing and purifying target protein; processing matrix serum, evaluating the standard, and evaluating a constant value and the uncertainty of the standard. The recombinant human alanine aminotransferase protein standard and the recombinant human aspartate aminotransferase protein standard prepared by adopting a gene recombination technique disclosed by the invention can be applied to clinical test of a laboratory and mass control of a kit.

The invention discloses an application of a mulberry polysaccharide extract in preparing a medicine or a health product capable of preventing or inhibiting chemical liver injury. The mulberry polysaccharide extract has relatively wide biological activity and high activity, and can obviously reduce the levels of alanine aminotransferase and aspartate transaminase in serum, effectively improve the activities of superoxide dismutase and glutathion peroxidase in the liver, and obviously reduce the content of glutathione and malonaldehyde. The mulberry polysaccharide extract can be used for preparing a medicine or a health product, in particular for preparing the medicine or the health product having an inhibiting or preventing effect for the chemical liver injury.

The invention discloses a kit for determining mitochondria aspartate aminotransferase, containing a reagent 1 and a reagent 2, wherein the reagent 1 contains aspartic acid protease, alpha- ketoglutaric acid, L-aspartic acid, malate dehydrogenase, lactate dehydrogenase, a preservative and PEG6000. The enzyme activity ratio of the aspartic acid protease to the malate dehydrogenase to the lactate dehydrogenase in the reagent 1 is (0.1-50):(0.1-50):(0.1-10); the ratio of the alpha- ketoglutaric acid to the malate dehydrogenase is (0.1-60)g:(0.1-50)KU; the mass ratio of the alpha- ketoglutaric acid to the L-aspartic acid to the preservative to the PEG6000 is (0.1-60):(5-200):(0.2-10):(1-100); and the mass ratio of NADH (Reduced Form of Nicotinamide-Adenine Dinucleotid) to a preservative to EDTA (Ethylene Diamine Tetraacetic Acid).2Na is (0.5-10):(0.2-10):(1-50). The kit for determining the mitochondria aspartate aminotransferase is used for determining the activity of the determining mitochondria aspartate aminotransferase, is free from the interference of high cell plasma aspartate aminotransferase and obtains accurate result.

The invention relates to an extraction method of a kiwi fruit root extract and an anti-liver injury application thereof, belonging to the fields of preparation and application technology of the kiwi fruit root extract. The invention takes kiwi fruit root or root bark as raw material, utilizes ethanol solution for extraction, further separates the extract on a column and extracts to obtain the kiwi fruit root extract containing active ingredients with anti-liver injury effect. The kiwi fruit root extract can significantly reduce alanine aminotransferase ALT in plasma of mice with acute carbon tetrachloride-induced liver injury, the decreasing range achieves 64.72 percent, which is greater than the effect of ammonium glycyrrhizinate, the kiwi fruit root extract can also significantly reduce serum aspartate aminotransferase AST, the decreasing range achieves 28.26 percent, which is not as good as the effect of the ammonium glycyrrhizinate. The invention provides a new valuable use for comprehensively utilizing the kiwi fruit root.

The invention is applicable to the field of Chinese patent medicines. A lipopolysaccharide-induced rat diffuse intravascular coagulation model is adopted for experimenting, and the experiment result shows that a compound thrombosis capsule can significantly inhibit the rise of liver-function biochemical indexes of aspartate aminotransferase and alanine aminotransferase, reduces the level of lipopolysaccharide-induced liver inflammation, and reduces congestion and hemorrhage phenomena in liver veins, namely finding out the new application of the compound thrombosis capsule playing a role of protecting the liver.

The invention relates to a method for producing sea cucumber bio-alcohol. The method has the following characteristics that: sea cucumber is soaked in water, the produced soak is centrifugally filtered and concentrated, the produced concentrated solution is cooled, fucoidan hydrolytic enzyme is added to enzymatically hydrolyze the fucoidan in the concentrated solution, the temperature is increased to 80 DEG C to 90 DEG C, so that the fucoidan hydrolytic enzyme is inactivated, the produced enzymatically hydrolyzed solution is filtered, edible alcohol and auxiliary materials are added and blended, and the produced sea cucumber bio-alcohol is filtered by diatomite, filled, sterilized and packed. The sea cucumber bio-alcohol can increase the pH value of the acidic body fluid of the human body, enhance the activity of insulin, repair islet cells injured by the acidic constitution, recover the normal functions of islet cells and effectively reduce postprandial plasma glucose; and the sea cucumber bio-alcohol can strengthen metabolism, improve the acidic constitution, stabilize blood pressure, reduce blood sugar, blood fat, blood viscosity and the like, prevent and improve diabetes complication, notably reduce the activity of liver-injuring serum alanine aminotransferase and aspartate aminotransferase, increase the activity of liver SOD and reduce the content of MDA, thus having the function of protecting the liver.

The invention discloses a blueberry liver protection beverage and a manufacture method of the blueberry liver protection beverage. The manufacture method comprises the steps of raw material pre-treatment, blueberry juice extraction, oriental wormwood, wild chrysanthemum, tuckahoe and houttuynia cordata active ingredient extraction, proportioning, sterilization, bottle loading and the like. Compared with the prior art, the method has the advantages that the blueberry juice yield can reach a value higher than 70 percent, the production cost is reduced, and the production period is shortened for two days. After the beverage product is drunk for a long time, the alanine amino transferase (ALT) in the human serum can be reduced by 20 to 55U / L, and the aspartate aminotransferase (AST) can be reduced by 10 to 25U / L, so the liver fibrillation and various kinds of chemical liver injury can be effectively prevented and reduced. The flavor of the product is harmonious, the pure, intense, elegant, harmonious and unique blueberry flavor is realized, the prepared blueberry juice beverage is subjected to microwave sterilization for 3 to 5 minutes, the browning of the blueberry juice beverage can be effectively prevented, and the quality guarantee period can reach 12 months, so the scaled and industrialized production can be realized.

The present invention discloses mutant aspartate aminotransferase and its preparation process and application. The mutant aspartate aminotransferase has the amino acid sequence as shown in SEQ IN No.2 with the 221st position of substituted Leu by Asn or the 338th position mutated from Phe to Lys. The mutant aspartate aminotransferase has raised biological function and is used in producing L-phenylalanine.

The invention discloses a composite stabilizer for an aspartate aminotransferase assay kit. The composite stabilizer comprises trihydroxymethyl aminomethane, disodium ethylenediaminetetraacetate, trehalose, gelatin, propylene glycol, a surfactant Brij-35 and a liquid biological preservative Proclin300. Through the technical scheme, the composite stabilizer for the aspartate aminotransferase assay kit comprises the components of a buffer solution, a stabilizer, a chelating agent, an organic solvent, a surfactant and a preservative and the like. After the composite stabilizer is added into the aspartate aminotransferase assay kit in a certain volume ratio, not only the accuracy of an assay result can be ensured, but also the stability thereof is higher, and the expiry date after the aspartate aminotransferase assay kit is opened can be effectively prolonged.

The invention discloses a water-soluble fullerene structure for preparing a medicine for treating fatty liver, and discloses a pharmaceutical composition and a method for treating fatty liver. Activeingredients for treating fatty liver are water-soluble fullerene, water-soluble inlaid metal fullerene, composition of the water-soluble fullerene and the water-soluble inlaid metal fullerene, and pharmaceuitical ester of above three and pharmaceutical salt of the three. The active ingredient water-soluble fullerene structure can reduce fatty vesicles in the liver, lower blood lipids, and obviously improve liver function, so that the content of alanine aminotransferase and aspartate aminotransferase tends to be normal, and the effect of treating fatty liver is highly effective. The active ingredient water-soluble fullerene structure can also regulate the level of redox in the liver.

The invention discloses a composite fungal intercellular polysaccharide composition with a liver protecting function and a preparation method. The composite fungal intercellular polysaccharide composition comprises 10-45 weight parts of coprinus comatus intercellular polysaccharide, 20-50 weight parts of white fungus intercellular polysaccharide and 20-45 weight parts of hohenbuehelia serotina intercellular polysaccharide. The preparation method comprises the steps of fermenting fungi, crushing the separated mycelia, adding enzyme for reacting, and separating solid and liquid phases; adding distilled water into the solid phase, then performing ultrasonic treatment, adding ethanol liquid, collecting and drying the sediment; concentrating the liquid phase, adding ethanol, standing, centrifuging to separate the sediment; combining the sediments obtained by solid and liquid phase treatment to obtain homogenous intercellular polysaccharides; and mixing the coprinus comatus intercellular polysaccharide, the white fungus intercellular polysaccharide and the hohenbuehelia serotina intercellular polysaccharide obtained by the method respectively to obtain the composite fungal intercellular polysaccharide composition with the liver protecting function. The composition has the advantages of effectively reducing the ALT (alanine aminotransferase) and AST (aspartate aminotransferase) level of serum, obviously reducing the malonaldehyde content of injured liver and obviously promoting biliary excretion so that the function of the injured liver can be recovered.

The invention discloses lactobacillus paracasei capable of relieving alcoholic intestinal injury and an application of the lactobacillus paracasei capable of relieving alcoholic intestinal injury, andbelongs to the technical field of microorganisms and medicines, The invention provides a medicine capable of preventing and / or treating alcoholic intestinal injury or alcoholic liver injury. Effective components of the medicine are lactobacillus paracasei CCFM1120, and the lactobacillus paracasei CCFM1120 can effectively relieve the alcoholic intestinal injury and the alcoholic liver injury. Thelactobacillus paracasei has the specific effects that the mRNA expression level of intestine tight junction proteins of alcohol modeled mice can be notably increased, the content of endotoxin in serumof the alcohol modeled mice can be notably reduced, the activity of alanine aminotransferase and aspartate aminotransferase in the serum of the alcohol modeled mice can be notably reduced, the oxidation stress level of the liver of the alcohol modeled mice can be notably reduced, and the pathology damage of liver tissue of the alcohol modeled mice can be notably improved.

The invention discloses application of adenosine and derivatives thereof in prevention and treatment of medicament-induced liver injury, relating to application of adenosine and derivatives thereof such as adenosine monophosphate, adenosine diphosphate and adenosine triphosphate in protection of acetaminophen-induced liver injury. A mouse model adopted in the application is an acetaminophen-induced mouse acute hepatic failure model, the dosage of the adenosine or derivatives thereof is 0.1 to 5mmol / kg, and the adenosine and derivatives thereof can reduce the activities of aspartate aminotransferase (AST) and alanine transaminase (ALT) in the medicament-induced liver injury, reduce the substantial cell necrosis area and improve the glutathione level so as to prevent and treat the acetaminophen-induced liver injury caused by liver toxic medicaments. Animal experiments prove that the adenosine and derivatives thereof have a significant effect on preventing and treating the medicament-induced liver injury, so the adenosine and derivatives thereof can be used for the preventing and treating processes of the medicament-induced liver injury, and a new method and means are provided for clinically treating the medicament-induced liver injury.