Quick detecting method for aspartate amino transferase vitality

Abstract

The present invention relates to electrochemical detection technology, and is quick detecting method of aspartate aminotransferase vitality. Electrochemical or optical change is produced corresponding to the vitality of the measured matter. The method includes: A. sucking the measured matter liquid drop into the reaction area between electric poles to generate oxidation-reduction on the surface of the electric poles so as to produce electron transfer and form current; and B. converting the aspartic acid into glutamic acid and oxaloacetic acid under the cooperated action of current and auxiliary factors and measuring the amount of glutamic acid and oxaloacetic acid so as to obtain the corresponding aspartate aminotransferase vitality. The present invention has raised response speed and detection sensitivity of current type enzyme sensor and lowered reaction voltage.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection, and is a rapid test method for detecting the activity of aspartate aminotransferase (Aspartate Aminotransferase, AST), also known as aspartate aminotransferase (Glutamine-Oxalacetate Transferase, GOT). Redox reactions occur on the electrode surface. This paper mainly introduces the detection method of biosensor for aspartate aminotransferase (AST) and the preparation method of sensitive reagent, which can quickly detect the activity of aspartate aminotransferase (AST). technical background [0002] In the just-concluded Athens Olympic Games, Chinese athletes worked hard and achieved good results that attracted the attention of the world. In addition to the hard work and sweat of the athletes, scientific training methods are also an important part of it. The evaluation of athletes' physical function status is the most critical part. A series of physiological and biochemical chan...

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Problems solved by technology

[0004] At present, there is no portable instrument product that uses electrochemical methods to detect aspartate aminotransferase (AST) on the market. Most of the detection equipment uses optical methods, which require high processing accuracy, high cleanliness, and portability. poor performance

Moreover, the traditional measurement method needs to draw a sufficient amount of blood from the forearm with a needle tube and separate the serum by centrifugation, which requires a large amount of blood, and the steps required are complicated and time-consuming, and the subjects feel a certain degree of discomfort

Specific to the production of sensor test strips, most of the current test strips and testing instruments rely on the thick film technology of screen printing. The production of sensor test strips using thick film technology is time-consuming and consumable. The electrodes obtained by screen printing method The surface of the belt is very uneven, the process is more complicated, and it is not as good as the thin film electrode based on the advanced microelectromechanical system (MEMS) manufacturing technology in terms of response time, sensitivity, stability, etc.

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