Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Dry chemical quantitative test strip for removing interference, gluten or aspartate aminotransferase quantitative test strip and detection method

An aspartate aminotransferase and dry chemical technology, which is applied in the field of dry chemical test strips and detection fields of aminotransferase, can solve problems such as easy occurrence of false positive results and the like

Active Publication Date: 2011-12-21
SINOCARE
View PDF16 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using the rate method (that is, testing the change rate of color development), removing endogenous interference requires more complicated optical instruments, often using optical devices such as integrating spheres, and the normal value of transaminase is generally very low, and alanine aminotransferase is 5U / L ~40U / L, aspartate aminotransferase is 8U / L~40U / L, and the normal reference value of pyruvate is relatively high, which is 65μmol / L (equivalent to the pyruvate produced by 65U / L transaminase in 1min). During the dry chemical test time (2min~3min), the color change rate of the chromogenic agent will also be affected by endogenous pyruvate to a certain extent, which is prone to false positive results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dry chemical quantitative test strip for removing interference, gluten or aspartate aminotransferase quantitative test strip and detection method
  • Dry chemical quantitative test strip for removing interference, gluten or aspartate aminotransferase quantitative test strip and detection method
  • Dry chemical quantitative test strip for removing interference, gluten or aspartate aminotransferase quantitative test strip and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] a kind of like figure 2 The shown dry chemical quantitative test strip for removing interference, the test strip includes a substrate 7, on which a sample application area 13 is fixed with an interference-removing reagent and a signal-providing reagent (in this embodiment, specifically includes a diffusion Layer 1, sample pad 2, blood filter membrane 3, enzyme binding pad 4 and flow pad 5), the sample loading area 13 is directly fixed on the substrate 7; the substrate 7 is also provided with a starting area fixed with a test reaction initiation reagent 14. The activation area 14 includes a reagent layer 6, the reagent layer 6 is connected to one end of the substrate 7 through an adhesive 8, and the adhesive 8 has a sufficient height to allow most of the reagent layer 6 to be suspended in the sample loading area. Zone 13 above. The sample application area 13 and the activation area 14 remain non-contact under normal conditions, and the activation area 14 (reagent layer...

Embodiment 2

[0060] a kind of like image 3 The shown dry chemical quantitative test strip for removing interference, the test strip includes a substrate 7, on which a sample application area 13 is fixed with an interference-removing reagent and a signal-providing reagent (in this embodiment, specifically includes a diffusion Layer 1, sample pad 2, blood filter membrane 3, enzyme binding pad 4 and flow pad 5), the sample loading area 13 is directly fixed on the substrate 7; the substrate 7 is also provided with a starting area fixed with a test reaction initiation reagent 14. The activation area 14 includes a reagent layer 6, the reagent layer 6 is connected to one end of the substrate 7 through an adhesive 8, and the adhesive 8 has a sufficient height to allow most of the reagent layer 6 to be suspended in the sample loading area. Zone 13 above. The sample application area 13 and the activation area 14 remain non-contact under normal conditions, and the activation area 14 (reagent layer ...

Embodiment 3

[0064] a kind of like Figure 4 Compared with the test strip provided in Example 1, the shown dry chemical quantitative test strip for removing interference does not have an enzyme binding pad 4, and the rest of the structure and application methods are the same as in Example 1 . Since the enzyme-binding pad 4 is not provided, substances originally immobilized on the enzyme-binding pad 4 (such as various catalytic reaction enzymes) can be directly immobilized on other structures in the sample loading area 13 (such as the flow pad 5 ).

[0065] The test strips of the above several embodiments, when cut into strips, may make the activation area contact with the sample application area, and the reagents fixed in the activation area to initiate the reaction may partially adhere to the sample application area. On the flow pad, the initiation reaction is uncontrollably advanced. This kind of problem can be solved by various methods, for example, an interlayer structure is provided...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses an interference-removing dry chemical quantitative test strip. The strip comprises a bottom liner, wherein the bottom liner is provided with a sample addition region and also a starting region; the sample addition region is fixed with interference-removing reagents and signal providing reagents; the starting region is fixed with test reaction starting reagents; and the sample addition region and the starting region are arranged on the test strip in a mode of maintaining a non-contact under a normal state and mutually contacting under a test state. The test method comprises the following steps of: adding a sample solution to be tested into the sample addition region, re-dissolving materials fixed on the sample addition region into the sample solution to be tested, and starting to perform a signal reaction to obtain a background signal value; and then enabling the starting region to contact with the sample addition region, re-dissolving materials fixed on the starting region, starting a test reaction, performing a second-time signal reaction on a product again, and estimating out a content of a biological enzyme to be tested according to a tested second-time characteristic value and the background signal value. The test method provided by the invention has the advantages of convenience for operation, low test cost, accurate test result and high test precision and can specifically be used in the alanine and aspartate aminotransferase quantitative test.

Description

technical field [0001] The invention relates to a dry chemical test strip and a test method, in particular to a dry chemical test strip and a detection method for transaminase. Background technique [0002] There are many interferences in dry chemical tests, such as the interference of uric acid and ascorbic acid in the determination of redox reaction, the interference of pyruvate in the detection of transaminase, especially the interference of pyruvate in transaminase is more serious. [0003] There are many kinds of transaminases, among which alanine aminotransferase (GPT / ALT) and aspartate aminotransferase (GOT / AST) are the most important. Alanine aminotransferase, also known as alanine aminotransferase, catalyzes the transamination between glutamic acid and pyruvate; of transamination. Clinically, the determination of these two transaminases in whole blood, serum, plasma, and interstitial fluid is a very important indicator in the diagnosis of heart disease, muscle dis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/52G01N33/558G01N21/31G01N21/76G01N21/64
Inventor 吉翔谢光李宗祥车宏莉
Owner SINOCARE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com