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Refined glossy privet fruit total glycosides capable of preventing and treating liver injury, preparation method and application thereof

A technology of Ligustrum lucidum and liver injury, which is applied to the field of refining total glucosides of Ligustrum lucidum and its preparation, can solve the problems of resource waste, environmental pollution, blackening of the ground, etc., and achieves reduction of MDA content, enhancement of GSH activity, and anti-liver injury. active effect

Inactive Publication Date: 2013-09-18
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Privet privet is also a common greening tree and street tree. Every autumn, a large number of mature privet privet seeds fall on the ground, blackening the ground, causing environmental pollution and waste of resources.
[0003] At present, there is no report on the extraction and separation of saponins that can prevent and treat liver damage from Ligustrum lucidum.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Refined total glycosides of Ligustrum lucidum with the effect of preventing and treating liver damage, which is prepared by the following method:

[0025] Take the dried Ligustrum lucidum, grind it, add 10 times the concentration of 50% ethanol to reflux and extract twice, each time for 2 hours, filter, combine the filtrate to recover ethanol under reduced pressure, put D101 macroporous resin on the filtrate, and use a concentration of 50% % ethanol elution, the eluent is decompressed and reclaims ethanol, and vacuum-dried to obtain 39% privetin, 17% privetin, 19% privetin Gl-13, 6% salidroside in percentages by weight Ingredients of Ligustrum lucidum refined total glycosides.

Embodiment 2

[0026] Example 2 Experimental Study on Anti-liver Injury of Carbon Tetrachloride in Rats by Refined Total Glycosides of Ligustrum lucidum

[0027] 1. Experimental method:

[0028] After feeding 60 rats normally for one week, they were randomly divided into 6 groups: normal control group (n=10), given normal feed; model group (n=10), given normal feed; positive drug intervention group (n=10) , give liver-protecting tablets (administered by intragastric administration with 150mg / kg liver-protecting tablets every day) and normal feed; the high, middle and low intervention groups (n=10×3 ), give respectively the refined total glycosides of Ligustrum lucidum 600mg / kg, 300mg / kg, 150mg / kg and normal feed prepared by embodiment 1. At the same time of administration, all groups except the normal control group were subcutaneously injected with 40% CCl4 (prepared with corn oil) on the 1st day, 4th day, 8th day, and 12th day at a dose of 4 mL / kg. On the evening of the last administratio...

Embodiment 3

[0035] Example 3 Experimental study on the effect of refined total glucosides of Ligustrum lucidum against alcoholic liver injury in mice

[0036] 1. Experimental method:

[0037]The mice were free to eat and drink, and were observed and adapted for one week before the experiment. Divide into 6 groups at random: normal control group (n=10), give normal feed; model group (n=10), fill with 58 degree Red Star Erguotou wine (0.2ml / l0g) every morning; positive drug intervention group (n= 10), give Hugan Tablets (1.0mg / 10g Hugan Tablets for intragastric administration every day) and 58-degree Red Star Erguotou Wine (0.2ml / l0g); the refined total glycosides of Ligustrum lucidum prepared in Example 1 of the present invention The high, medium and low intervention groups (n=10×3) administered 1.0 mg / 10 g, 0.5 mg / 10 g, 0.25 mg / 10 g, and 58-degree red star glycosides prepared in Example 1 by intragastric administration, respectively. Erguotou wine (0.2ml / l0g). After 30 days, the mice w...

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Abstract

The invention discloses refined glossy privet fruit total glycosides capable of preventing and treating liver injury and a preparation method thereof. The preparation method is characterized in that the chemical compositions and pharmacological activity of the glossy privet fruit are researched and selected systematically; and the experiment results show that the selected refined glossy privet fruit total glycosides can obviously reduce serum ALT (Alanine Transaminase) and AST (Aspartate Aminotransferase) activities of a CCl4-induced liver injury model mice and the MDA (Methyl Di Aldehyde) content in the hepatic tissue of an alcohol-induced liver injury model mice and improve the GSH (Glutathione) activity of the liver, and also show that the refined glossy privet fruit total glycosides provided by the invention not only can obviously protect the liver from chemical liver injury and long-term drinking induced liver injury and can be developed into novel medicines or health products for preventing liver injury.

Description

technical field [0001] The invention relates to a plant extract, in particular to refined total glycosides of Ligustrum lucidum with the functions of preventing and treating liver damage, its preparation method and application. Background technique [0002] Ligustrum lucidum is the dry ripe fruit of the plant Ligustrum privet of Oleaceae. Harvest when the fruit is ripe in winter, remove branches and leaves, steam or put in boiling water for a while, and then dry; or directly dry. Its nature and flavor are sweet and bitter, cool, human liver and kidney meridians, and it is a medicinal material commonly used with the same origin as medicine and food. Modern research shows that Ligustrum lucidum mainly contains chemical components such as triterpenoids, split ring iridoid glycosides and phenylethanol glycosides, which can improve the body's immune function, anti-aging, and protect nerve cells from hypoxia / glucose damage. and other pharmacological effects. Privet privet is al...

Claims

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Application Information

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IPC IPC(8): A61K36/638A23L1/29A61P1/16A23L33/00
Inventor 杨念云段金廒唐于平钱大玮
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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