156results about How to "Broad biological activity" patented technology

A method for using ganoderma lucidum liquid fermentation liquor to prepare feed and feed additive containing ganoderma lucidum active ingredient, pertains to the field of biofermentation and feed. The invention includes steps of: a. centrifugally separating the ganoderma lucidum liquid fermentation liquor at 5000 per-minute to obtain the ganoderma lucidum fermentation supernatant and ganoderma lucidum residues; b. adopting a rear spraying process to mixing the ganoderma lucidum fermentation supernatant with the feed in a proportionality of adding 0.5-1.5 ml of the supernatant in the feed for preparing feed containing ganoderma lucidum active ingredient; c. performing a second fermentation to the ganoderma lucidum residues for preparing the feed additive containing ganoderma lucidum active ingredient. The invention fully use the ganoderma lucidum fermentation liquor, has no waste water and waste slag discharge, simple steps, low cost, and easy large-scale industrialization. Products are rich in Ganoderma lucidum polysaccharides and ganoderma lucidum acid; have a balance immune system, and antibacterial, antiviral, anti-parasitic, anti-tumor, anti-radiation and the like extensive physiology activities, thereby largely improving the immune disease resistance, maintaining the animal intestinal health, and further improving the production performance and market efficiency of animals.

The invention relates to an imidazoline asymmetrical bi-quaternary ammonium salt. The preparation method comprises the following steps that: diethylene triamine and organic acids of octalkyl to heptadecyl react to generate alkyl imidazoline; long chain alkyl tertiary amine, hydrochloride of long chain alkyl tertiary amine and epichlorohydrin react to generate N-(3-chlorine-hydroxypropyl)-N, N-dimethyl long chain alkyl quaternary ammonium salt; and the alkyl imidazoline and the N-(3-chlorine-hydroxypropyl)-N, N-dimethyl long chain alkyl quaternary ammonium salt react. When the salt is used as a carbon steel corrosion protection acid pickling inhibitor, the salt has the advantages of easy synthesis, low-priced and easily-bought materials; because the salt is the organic inhibitor, the salt is nontoxic and unharmful, and has wide biological activity and good water solubility and can effectively inhibit corrosion of metallic matrix in the acid and excessive consumption of acid liquor; moreover, the salt also has the advantages of low consumption, high efficiency, strong sustained action capability and good application prospect.

The present invention relates to preparation of a blattodea polypeptide, and uses of the blattodea polypeptide in anti-gram-positive bacteria and anti-gram-negative bacteria. Specifically the present invention relates to a blattodea extract effective fraction having a function of prevention and treatment of bacterial infection diseases, a preparation method and a medical use thereof. The Periplaneta Americana extract effective fraction of the present invention is a polypeptide substance with a molecular weight less than 5000 dalton, and is prepared by the following steps: carrying out solvent extraction and membrane separation on fresh polypide or dried polypide of Periplaneta Americana to obtain the product of the present invention, wherein the polypeptide active substance of the effective fraction has significant anti-gram-positive bacteria activity and anti-gram-negative bacteria activity, can be prepared into forms of a hydrogel, a cataplasm, lyophilized powder, a water agent, an aerosol, a suppository, a film agent, an external application liniment, and an ointment, and can be used for preparations of drugs for prevention and treatment of diseases related to gram-positive bacteria and gram-negative bacteria and infections, daily chemical products or medical devices.

The invention relates to a traditional Chinese medicine microecological preparation for improving the immunity of chicks, and a preparation method and usage of the traditional Chinese medicine microecological preparation, belonging to the technical field of veterinary traditional Chinese medicine microecological preparations. The traditional Chinese medicine microecological preparation is prepared by carrying out mixed fermentation on fermentation strains and the following raw materials: 15-20 parts of radix astragali seu hedysari, 5-10 parts of Chinese angelica, 5-10 parts of fructus ligustri lucidi, 5-10 parts of radix isatidis, 5-10 parts of folium isatidis, 15-20 parts of cortex eucommiae, 5-10 parts of fructus crataegi, 5-10 parts of malt, 1-2 parts of brown sugar, 1-3 parts of fructo-oligosaccharide and 1-3 parts of lysine; the fermentation strains comprise a bacillus subtilis strain, a lactobacillus plantarum strain, an enterococcus faecium strain and saccharomyces cerevisiae. The traditional Chinese medicine microecological preparation can enhance the immunity of the chicks and improve the digestion-absorption function of the intestinal tracts of the chicks, thus achieving the aims of increasing the disease resistance of the chicks as well as reducing the morbidity and mortality of the chicks on the whole, and increasing the economic benefit.

The invention provides a functional drink with a function of relieving visual fatigue and a preparation method thereof, belonging to the technical field of functional drinks. The functional drink with the function of relieving the visual fatigue is prepared from five raw materials of lutein, medlar, chrysanthemum, white granulated sugar and citric acid, wherein the lutein is extracted from marigold; the functional drink is prepared by adopting a series of steps of pretreating raw materials, extracting, blending and the like. The functional drink prepared by adopting the preparation method is safe and reliable in raw materials; meanwhile, no caffeine, preservative and artificial color are added, and the functional drink has functions of improving the eyesight and relieving the visual fatigue.

The invention provides an application of a mangiferin compound with a structure shown as a formula I as a pancreatic lipase inhibitor, an application of a mango extract containing the mangiferin compound with the structure shown as the formula I as a pancreatic lipase inhibitor, and an application of the mangiferin compound with the structure shown as the formula I or the mango extract containing the mangiferin compound with the structure shown as the formula I to preparation of a medicament or a food for treating and / or preventing obesity, and provides an application of the mangiferin compound with the structure shown as the formula I or the mango extract containing the mangiferin compound with the structure shown as the formula I to preparation of a medicament or a food for treating and / or preventing obesity. The mangiferin compound has the function of inhibiting the activity of pancreatic lipase, so that the occurrence of obesity can be avoided by inhibiting the accumulation of in-vivo fat. Meanwhile, the mangiferin compound has wide physiological activity, so that side effects on human bodies caused by the conventional weight-losing product can be avoided.

A seaweed sausage is prepared from seaweed, lean and fat pork, edible salt, starch, plant oil, spirit scallion, fresh ginger, powdered spices and sodium nitrite through cutting seaweed to become dust, mincing pork, proportionally mixing them with others, filling the mixture in sausage casing, drying, and fumigating at 75-85 deg.C for 50-90 min.

The invention discloses a combretastatin compound and a preparation method. The preparation method of the combretastatin compound of the invention comprises the following steps: dissolving 3'-amino combretastatin and N-(1-oxyl-2,2,6,6,-tetramethyl-oxygen-carbonyl)-L-amino acid in dried dichloromethane, uniformly stirring under argon protection, adding dicyclohexylcarbodiimide and 1-hydroxybenzotriazole, stirring and reacting under argon protection, filtering and removing white precipitates after the reaction, removing the solvent by distillation to obtain a crude product, purifying the crude product by column chromatography, and eluting the product by petroleum ether and ethyl acetate liquid with a volume ratio of 10:1-5:1 to obtain the target product. The combretastatin compound of the invention is applicable to the preparation of anticancer medicaments, and is especially applicable to the preparation of medicaments for treating leukemia, liver cancer, gastric cancer, and cervical cancer.

The invention provides sunscreen cream with double-isolation and post-basking repairing functions. The sunscreen cream comprises the following effective components in parts by weight, 45-55 parts of water, 10-14 parts of physical sunscreen agents, 8.1-11.9 parts of emollient, 3-5 parts of emulsifiers, 11.7-15.6 parts of chemical sunscreen agents, 1.5-6.1 parts of skin conditioners, 0.4-0.6 part of preservatives, 0.4-0.6 part of thickening agents, 2-3 parts of PH (potential of hydrogen) conditioning agents, 0-0.2 part of flavoring agents and 0.2-0.3 part of complexing agents. According to the sunscreen cream, the physical sunscreen agents and the chemical sunscreen agents are scientifically matched, UVA (ultraviolet A) and UVB (ultraviolet B) are effectively stopped, the sunscreen cream can prevent skins from being tanned and effectively protect the skins from sunburn, the skin conditioners are added into the sunscreen cream, and the post-basking repairing function is achieved.

The invention provides anapplication of chitosan oligosaccharide and a vaccine containing the chitosan oligosaccharide. The chitosan oligosaccharide is applied to the vaccine as an adjuvant, namely each single vaccine contains 2.5-10mg of chitosan oligosaccharide. The chitosan oligosaccharide provided by the invention is a chitosan oligosaccharidemixturewhich is formed by 2-10 glucosamines and of which the molecular weight is smaller than 3000, and is small in toxic or side effect, and is safe and reliable when being used in an immunizing dose range; antigen-specifichumoral immune responsecan be effectively induced; the induced humoral immune response effect is superior to that of a group free of an adjuvant; and the chitosan oligosaccharide is available in raw materialwhich is a commercially available product, is simple in preparation technology, low in cost, stable in performance, relatively high in biological potency, and free of a toxic or side effect, and can be add to a plurality of traditional vaccines or genetic engineering vaccines as the adjuvant.

The invention discloses an anti-acne composition containing epidermal growth factors and tea tree oil and a preparation method thereof. The anti-acne composition disclosed by the invention comprises the following components: epidermal growth factors, tea tree oil, metallothionein, chlorhexidine, humectant, tackifier, alpha-carboxylic acid, pH value regulator, surfactant and deionized water. The preparation method of the anti-acne composition disclosed by the invention comprises the following steps of: mixing and heating the epidermal growth factors, the tea tree oil, the metallothionein, the chlorhexidine and the surfactant to be used as an oil phase; dissolving and heating the humectant, the tackifier and the alpha-carboxylic acid in water to be used as a water phase; adding the oil phase into the water phase, and stirring uniformly; and adding the pH value regulator for regulating the pH value, thereby obtaining the anti-acne composition containing epidermal growth factors and tea tree oil. The epidermal growth factors contained in the anti-acne composition disclosed by the invention can accelerate the metabolism of skin cells, and the natural tea tree oil capable of resisting bacteria and diminishing inflammation can efficiently sterilize bacteria, diminish inflammation and remove acnes, thus the anti-acne composition has good moisture retention and small skin irritation.

The invention discloses an application of a mulberry polysaccharide extract in preparing a medicine or a health product capable of preventing or inhibiting chemical liver injury. The mulberry polysaccharide extract has relatively wide biological activity and high activity, and can obviously reduce the levels of alanine aminotransferase and aspartate transaminase in serum, effectively improve the activities of superoxide dismutase and glutathion peroxidase in the liver, and obviously reduce the content of glutathione and malonaldehyde. The mulberry polysaccharide extract can be used for preparing a medicine or a health product, in particular for preparing the medicine or the health product having an inhibiting or preventing effect for the chemical liver injury.

The invention relates to a walnut-tuckahoe oral liquid with function of providing assistance to improving memory and the production method thereof. The active ingredients in each 1000ml of the oral liquid comprise 100 to 180g of walnut kernel, 80 to 150g of rhizoma polygonati, 80 to 120g of tuckahoe, 80 to 150g of wheat, 30 to 60g of Chinese date, 4 to 6g of taurine, 1.5 to 2g of zinc gluconate, 0.05 to 0.1g of vitamin B1, 80 to 120g of white sugar and 0.2g of potassium sorbate. The oral liquid is prepared after procedures of extraction, condensation, ethanol precipitation, ethanol recovery, filtration, mixing, fine filtration, diluting to volume, encapsulation, sterilization, light inspection, packing, etc are carried out. The formula of the oral liquid is preferably selected according to the modern medical theory and treatment based on syndrome differentiation in the traditional Chinese medicine, thus the formula is scientific and reasonable, and the oral liquid can be conveniently taken, and is safe and reliable.

The invention relates to a 3'-phenylspiro[indoline-3,2'-pyrrolidin]-2-one derivative and its preparation method and application, and belongs to the technical field of heterocyclic compounds. The three components of acetyl coumarin compounds, indolequinone compounds and amino acids are used as the main raw materials. The three components undergo a cyclization reaction to form a spiro ring structure. The spiro ring structure then reacts with the corresponding nitrogen-containing fragment to form a spiro ring. Derivatives, namely 3'-phenylspiro[indoline-3,2'-pyrrolidin]-2-one derivatives. The present invention is applied to the preparation of anti-cancer drugs such as anti-non-small cell lung cancer and colon cancer drugs, and has the advantages of high yield, anti-tumor cell value-proliferating activity and other advantages.

The invention provides a method for preparing marine plant alginate polysaccharide enteric coated hard capsules. The method is characterized by comprising the following steps of: weighing sodium alginate, adding deionized water for stirring and dissolving the sodium alginate to prepare hydrosol with concentration of between 0.1 and 30% by mass percentage; adding glycerol with purity of more than 96% to ensure the concentration of the glycerol is between 0.1 and 10% by mass percentage; uniformly stirring, and defoaming; immersing die rods of capsule dies of between No.0 to 5 into the defoamed glycerol for 4 to 15 seconds; picking up the die rods, turning over the die rods for 3 to 5 times, and coagulating the die rods in a coagulating bath; drying under a constant-temperature constant humidity condition, and pulling out casings to prepare the alginate polysaccharide enteric coated hard capsules. The marine plant alginate polysaccharide enteric coated hard capsules prepared by the method have the advantages of excellent membrane forming property, sustained release property, safety, stability, biocompatibility, biodegradability and the like; and the quality and safety of the capsules are improved.

The invention discloses 3-(4-phenyl-1H-imidazolyl-5-yl)-1H-indole derivatives, and a preparation method and application thereof, belonging to the technical field of heterocyclic compounds. The N-piperidyl formacyl group with unique biological activity is creatively introduced into the imidazole-indole system, and the halogen atom with unique biological activity is introduced into the 3-(4-phenyl-1H-imidazolyl-5-yl)-1H-indole system, so that the synthesized 3-(4-phenyl-1H-imidazolyl-5-yl)-1H-indole derivatives have favorable anti-tumor cell proliferation activity. The 3-(4-phenyl-1H-imidazolyl-5-yl)-1H-indole derivatives can be further modified to be used as a novel antineoplastic drug, have favorable anti-non-small cell lung cancer (A549) proliferation activity, and can be used as a new drug for resisting non-small cell lung cancer and human colonic adenocarcinoma.

The invention relates to 5-(1H-indolyl-3-methylene)-1,3-thiazolidinyl-4-one derivatives, and a synthesis method and application thereof. By using ethanol and / or water as a solvent, substituted 2-substituted-imino-1,3-thiazolidinyl-4-one and 1H-indolyl-3-formaldehyde are subjected to reflux reaction under the catalytic condition of piperidine through intermolecular dehydration condensation reaction to form methylene linking group, thereby obtaining the 5-(1H-indolyl-3-methylene)-1,3-thiazolidinyl-4-one derivatives. The intermediate 2-substituted-iminothiazolidinyl-4-one is prepared by carrying out cyclization reaction on various monosubstituted ethyl thiocarbamide chloroacetates or chloroacetic acids in a low-boiling solvent under reflux conditions, and the intermediate 2-substituted-imino-3-substituted-1,3-thiazolidinyl-4-one is prepared by carrying out a green environment-friendly synthesis technique on various disubstituted symmetric thiocarbamides and chloroacetic acids. The bioactivity preliminary screening experiment result of all the target compounds on the enzyme molecular level indicates that the target products have certain inhibition activity on PTP1B and CDC25B to different degrees.

Relating to the daily chemical field and the pharmaceutical industry, the invention in particular provides a chitosan chlorogenic acid salt, a preparation method and application thereof. The chitosanchlorogenic acid salt has a structural formula shown as the specification, wherein the average degree of polymerization n is 10-3000. The preparation method includes: dissolving chlorogenic acid at high temperature, then carrying out salt forming reaction with chitosan directly to obtain the chitosan chlorogenic acid salt. And the molar quantity of chlorogenic acid is 1-2 times that of chitosan. The biological activity like antioxidant activity of the chitosan chlorogenic acid salt is greatly improved, the preparation raw materials are easily available, the preparation method is simple, the conditions are mild, and the chitosan chlorogenic acid salt can be developed into an antioxidant and widely applied in biology, medicine, food, chemical industry and other fields.

The invention discloses glucosamine chlorogenate as well as a preparation method and application thereof. The preparation method comprises the following steps of by taking glucosamine hydrochloride, sodium methoxide and chlorogenic acid as raw materials, performing neutralisation and desalination on the glucosamine hydrochloride with a sodium methoxide alkaline solution to obtain a glucosamine solution, and performing reaction on the glucosamine solution and the chlorogenic acid to obtain the glucosamine chlorogenate. The prepared glucosamine chlorogenate is greatly improved in the biologicalactivity such as antioxidant activity, available in preparation raw materials, simple to prepare and mild in condition, can be developed into an antioxidant, can be widely used for the field of biology, medicines, foods, chemical engineering and the like and lays a foundation of further application and development of the glucosamine.

Acidichromism is a color changing form which grows fast in recent years. Although more studies on acidichromism performance of polymers appear, and the relation between the acidichromism performance and solvent and the relation between the acidichromism performance and acid strength are reported, the relation between the acidichromism and a polymer structure are not reported yet. Therefore, according to the invention, a novel conjugated polymer is successfully compounded through Suzuki reaction by taking a pyrimidine monomer of a 2-amidogen substituent as a copolymer electron-deficiency group and taking a benzene ring as an electron-rich group, the polymer is subjected to structural characterization and performance study, the impact of a pyrimidine unit substituent on the acidichromism performance of pyrimidine and benzene alternating copolymers is discussed, further, the impact of an alternating unit (a benzene unit) on the acidichromism performance of pyrimidine copolymers is emphatically discussed, and therefore, the invention provides a novel polymer in the acidichromism performance and determines the acidichromism scope and the lowest responsive acid concentration, and the structure formula of the acid-doped representative polymer is shown in a diagram of the abstract.

The present invention aims to provide a lactoferrin fusion protein, which is configured to retain the biological activities of natural lactoferrin, to have a significantly prolonged in vivo lifetime, and to be more clinically useful than natural and gene recombinant lactoferrin, as well as a method for preparation thereof, etc. The present invention provides a fusion protein formed with a protein or peptide comprising an FcRn-binding region and lactoferrin or a biologically active fragment or peptide of lactoferrin, which is represented by:(LF-s-Y)n or (Y-s-LF)n [wherein LF represents lactoferrin or a biologically active fragment or peptide of lactoferrin, represents the protein or peptide comprising an FcRn-binding region, s represents ally amino acid sequence of 0 to 10 residues, and n represents an integer of 1 to 10], or a variant thereof.

The invention discloses dendrobium officinale polysaccharide / astragalus polysaccharide composite hydrogel as well as a preparation method and application thereof. The preparation prescription of the dendrobium officinale polysaccharide / astragalus polysaccharide composite hydrogel takes composite polysaccharide of dendrobium officinale polysaccharide and astragalus polysaccharide as a main medicine. The preparation comprises the following components in percentage by mass: 1%-2% of the main medicine, 0.5%-1.8% of a gel matrix, 5%-15% of a skin penetration promoting agent, 5%-10% of a humectant, 1%-5% of an antioxidant, 0.1%-0.2% of a preservative and the balance of water, and the pH value of the solution is regulated by a pH regulator. According to the composite hydrogel disclosed by the invention, polysaccharide extracted from natural plants dendrobium officinale and astragalus membranaceus is utilized, an anti-inflammatory and anti-aging skin care product external preparation is researched, and pharmacological experiments prove that the product disclosed by the invention has the curative effects of resisting bacteria, diminishing inflammation, whitening, moisturizing, resisting aging, nourishing skin and the like. A ball milling method is adopted to assist extraction, effective active ingredients of dendrobium officinale are reserved to the maximum extent, the cost is reduced, the method is easy to operate, and industrial production is easy to achieve.

The present invention relates to preparation of a blattodea polypeptide, and uses of the blattodea polypeptide in anti-gram-positive bacteria and anti-gram-negative bacteria. Specifically the present invention relates to a blattodea extract effective fraction having a function of prevention and treatment of bacterial infection diseases, a preparation method and a medical use thereof. The Periplaneta Americana extract effective fraction of the present invention is a polypeptide substance with a molecular weight less than 5000 dalton, and is prepared by the following steps: carrying out solvent extraction and membrane separation on fresh polypide or dried polypide of Periplaneta Americana to obtain the product of the present invention, wherein the polypeptide active substance of the effective fraction has significant anti-gram-positive bacteria activity and anti-gram-negative bacteria activity, can be prepared into forms of a hydrogel, a cataplasm, lyophilized powder, a water agent, an aerosol, a suppository, a film agent, an external application liniment, and an ointment, and can be used for preparations of drugs for prevention and treatment of diseases related to gram-positive bacteria and gram-negative bacteria and infections, daily chemical products or medical devices.

The invention relates to a compound recipe kidney-strengthening tablet for enlivening the spleen and strengthening kidney in the traditional technique field. The preparing method comprises the following steps: disintegrating rhubarb horsetails into the powder; collating after immersing; collecting the collation liquid; disintegrating radix salvia miltiorrhiza, astragalus root, extractum glycyrrhizae liquidum and hirudo into the powder; extracting with alcohol reflux; filtering; merging the filtering liquid and the collation liquid; mixing uniformly; concentrating into the dense plaster; getting the extract of five tastes medicine; adding fermenting Cordyceps sinensis bacterial powder, crystalline cellulose, sodium carboxymethylstarch, starch and dolomol; mixing uniformly; getting the product by pelleting. The invention can reduce BUN and SCr level, which improves plasma protein content, improves the nutrient state, accelerates the renovation and the reactivation of nephric tubule epithelial cell, corrects the metabolic disorder of amino acid, protein and lipid, adjusts the cell immunity, improves the immunity, protects and recovers the kidney function, and prevents secondary infection.

The invention provides a rabbit feed additive and rabbit feed using the additive, which can solve the problem that a traditional meat rabbit has strong offensive smell, and can avoid using antibiotics during a meat rabbit breeding process, so that the bred meat rabbit product is fresh, tender and firm in meat quality with high resilience. The rabbit feed additive is prepared from the following raw materials in parts by weight: 2000 to 2200 parts of folium artemisiae argyi, 2000 to 2200 parts of pine needle, 1500 to 2000 parts of folium isatidis, 1000 to 1500 parts of pericarpium citri reticulatae, 1000 to 1500 parts of herba houttuyniae, 800 to 1200 parts of herba andrographitis, 800 to 1200 parts of radix isatidis, 600 to 800 parts of radix sophorae flavescentis, 600 to 800 parts of radix astragalis, 600 to 800 parts of herba portulacae, 600 to 800 parts of herba taraxaci, 500 to 800 parts of radix astragalis, 500 to 1000 parts of radix glycyrrhizae, 500 to 1000 parts of folium perillae, 500 to 1000 parts of garlic, 500 to 800 parts of cassia bark, 250 to 500 parts of flos caryophylli, 250 to 500 parts of fructus anisi stellati, 300 to 400 parts of fresh ginger, 300 to 400 parts of radix angelicae sinensis,300 to 400 parts of herba menthae,300 to 400 parts of perilla nankinensis, 300 to 400 parts of cortex phellodendri, 300 to 400 parts of rhizoma atractylodis, 300 to 400 parts of radix sophorae flavescentis, 300 to 400 parts of herba portulacae, 300 to 400 parts of herba taraxaci, 300 to 400 parts of laminaria japonica root, 250 to 500 parts of fructus anisi stellati, and 200 to 250 parts of pepper.

The invention discloses a method for performing cyclized synthesis on multi-substituted indole through elemental-iodine promoted enaminone. The method comprises a step of performing intramolecular cyclization on the enaminone under conditions that low-cost and low-toxicity elemental iodine is used as a promoting agent and cesium acetate is alkali so as to generate the multi-substituted indole. According to the method disclosed by the invention, the use of a transitional metal catalyst is not needed, and addition of ligands is not needed, so that an operation is simple, a reaction product is single, and the atomic economy is high; and moreover, the product is only subjected to simple column chromatographic separation after the reaction is carried out, so that the yield of the product is high, and the obtained multi-substituted indole has wide biological activity and has a medicinal value.

The invention discloses a carbazole indolequinone derivative, which is a compound with a brand new structure and has good fluorescence characteristic and medicinal value. The invention further provides a preparation method of the carbazole indolequinone derivative. The derivative is synthesized from an indolequinone compound and aromatic amine in one step under the action of a solvent, a catalystand an alkali. The raw materials are easy to obtain, the operation is simple, the synthesis is convenient and rapid, and the industrial production is easy to realize. The invention also provides an application of the carbazole indolequinone derivative, and the carbazole indolequinone derivative is used as a fluorescent material or a medicinal material to be applied to various fields and has a goodapplication prospect.

The invention discloses a method for evaluating hair regeneration effect of lyophilized powder by measuring cytokine content, which relates to the field of biological engineering. The method comprisesthe steps of preparation of lyophilized powder, hair follicle-related cell culture, co-culture of the lyophilized powder and hair follicle-related cells, and the collection and detection of the cellsand a supernatant after culture. The evaluation cells selected by the invention are all related to the occurrence and development of hair follicles, the method is indirectly feasible and can be usedfor evaluating the hair regeneration effect of the product in vitro, has the advantages of simple operation and strong repeatability, and can be used as the effective evaluation method for quality inspection of subsequent products.

The invention discloses a method for preparing and identifying a recombinant bifidobacterium vaccine of a taenia solium TSO45W-4B gene. The taenia solium TSO45W-4B gene is synthetized by a complete genome, and directionally cloned to an escherichia coli-bifidobacterium expression vector pGEX-1lambdaT; a recombinant plasmid pGEX-TSO45W-4B is built; bifidobacterium longum (B.longum) is transformed in a manner of electroporation, and a taenia solium recombinant Bb(pGEX-TSO45W-4B) vaccine is built. Thus, a valuable novel vaccine is provided for prevention and treatment of cysticercosis cellulosae.

The invention provides a synthesis method of a benzoxepine compound. A diarylether compound is efficiently synthesized through a reaction of a diaryl cyclic iodonium salt compound and a substrate containing a hydroxyl group, and the benzoxepine compound is obtained through intramolecular cyclization. The reaction operation is simple, the post-treatment is convenient, and the problems of low efficiency, harsh reaction conditions and the like of a conventional synthesis method are solved; the atom economy of the substrate is fully utilized; and the diarylether compound is efficiently obtained byusing the hydroxyl-containing compound and the diaryl cyclic iodonium salt at a certain temperature under the catalysis of cheap metal copper, intramolecular cyclization is carried out through further reaction, the highest yield of the obtained diarylether compound is 99%, and the highest yield of the benzoxepine compound is 95%.