The invention belongs to the technical field of
plant tissue culture and particularly relates to a factory
seedling cultivating method of
actinidia arguta. The method is mainly technically characterized by comprising the five steps of: preparing culture media, selecting and disinfecting explants, inoculating, subculturing and
transplanting in a sand
bed. The factory
seedling cultivating method of
actinidia arguta is mainly provided on account of the conditions that
actinidia arguta is in a wild state in all northeast areas, and the traditional cultivating
modes which comprise
sowing propagation, cuttage propagation and
grafting propagation cause the problems that the variation of
offspring characters is serious, male plants are difficult to separate from female plants, resources are seriously damaged, the
seedling cultivating speed is low, the characters of the female plants are difficult to keep, the plants are easily infected with
bacteria, the
survival rate of the seedlings is low, and the like. Induced buds are subcultured and then directly transplanted in the sand
bed, the rooting culture process is omitted, the seedling cultivating period is shortened by 50-60 days, the seedling cultivating cost is greatly lowered, the
survival rate of the seedlings is obviously increased, the
plant infection rate is reduced, excellent female
plant characters are kept, and the variety degrading problem is solved.