Method for evaluating hair regeneration effect of lyophilized powder by measuring cytokine content
A technology of cytokines and freeze-dried powder, applied in the field of bioengineering, can solve the problems of no reference method, time-consuming and labor-consuming, unfavorable efficacy comparison, etc.
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[0031] Example: such as figure 1 , figure 2 with image 3 As shown, a method for evaluating the hair growth effect of freeze-dried powder by cytokine content assay, its steps are as follows:
[0032] Step 1: Preparation of freeze-dried powder
[0033] 1) Primary separation of ADSCs: Obtain adipose tissue by liposuction at 1500r / min, centrifuge at room temperature for 10min, remove the liquid part, and obtain solid fat particles. Add an equal volume of saline to wash and centrifuge. Add an equal volume of 200U / ml collagenase to the solid fat particles, digest at 37°C for 60-90min with constant temperature shaking, until no fat particles are visible to the naked eye, collect the digestive juice, centrifuge at 1000r / min for 10min at room temperature, remove the supernatant, and take Add 10ml of normal saline to the cell pellet, centrifuge at 1000r / min for 5min at room temperature, wash the cells, take the cell pellet, resuspend with 3ml of culture medium, count and inoculate...
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