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Preparation of blattodea polypeptide, and uses of the same in anti-gram-positive bacteria and anti-gram-negative bacteria

An application and technology of antibacterial active substances, applied to the effective parts of cockroach extract and the field of preparation thereof, can solve problems such as lack of broad-spectrum antibacterial, and achieve the effects of being beneficial to industrialization, potential social and economic benefits, and high yield

Inactive Publication Date: 2013-11-06
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After investigation, it was found that in the past, there were no related reports on the preparation of pharmaceutical preparations prepared from the extract obtained by the membrane separation method of the ethanol extract of Periplaneta americana as the main drug, and there were no literature reports on the use of the above preparations for broad-spectrum antibacterial

Method used

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  • Preparation of blattodea polypeptide, and uses of the same in anti-gram-positive bacteria and anti-gram-negative bacteria
  • Preparation of blattodea polypeptide, and uses of the same in anti-gram-positive bacteria and anti-gram-negative bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 : Preparation of Periplaneta americana aqueous extract enriched by ultrafiltration membrane separation method for effective fractions of polypeptides with a molecular weight less than 5KDa (sample A)

[0028] 1.1 Preparation of Periplaneta americana Water Extract

[0029] Add 3 kg of Periplaneta americana medicinal materials, add 10,000 ml of double distilled water, soak and extract once at 45°C for 24 hours; soak and extract once at 50°C for 24 hours; soak and extract once at 55°C for 24 hours. The extracts were combined, concentrated to dryness under reduced pressure, and dried in vacuum to obtain the aqueous extract of Periplaneta americana.

[0030] 1.2 Preparation of sample A by 5KDa ultrafiltration membrane filtration orthogonal test

[0031] After a single factor test on the factors affecting the membrane separation effect, it is determined that the influencing factors are rotational speed, time, pH value, concentration ratio, and finally determine th...

Embodiment 2

[0057] Example 2 : Preparation of Periplaneta americana ethanol extract by ultrafiltration membrane separation method to enrich effective fractions of polypeptides with a molecular weight less than 5KDa (sample C)

[0058] 2.1 Preparation of Periplaneta americana alcohol extract

[0059] Add 3 kg of Periplaneta americana medicinal materials, add 10,000 ml of 55% ethanol, soak and extract at room temperature, extract 3 times, each time for 24 hours. The extracts were combined, concentrated to dryness under reduced pressure, and dried in vacuum to obtain the alcoholic extract of Periplaneta americana.

[0060] 2.2 Preparation of peptide active substance (sample C) with molecular weight <5KDa

[0061] According to the extraction parameters optimized by the orthogonal test in Example 1, take 300 mg of the Periplaneta americana ethanol extract prepared in 2.1 and dissolve it in 1000 ml of warm water; In the ultrafiltration device of the ultrafiltration membrane, perform ultrafi...

Embodiment 3

[0066] Example 3 : The preparation of the acetone-water extract of Periplaneta americana to enrich the active parts of polypeptides with a molecular weight less than 5KDa by ultrafiltration membrane separation (sample D)

[0067] 3.1 Preparation of acetone-water extract of Periplaneta americana

[0068] Add 3 kg of Periplaneta americana medicinal materials, add 10,000 ml of 70% acetone aqueous solution, soak and extract at room temperature, extract 3 times, each time for 24 hours. The extracts were combined, concentrated to dryness under reduced pressure, and dried in vacuum to obtain the acetone-water extract of Periplaneta americana.

[0069] 3.2 Preparation of polypeptide sample (sample D) with molecular weight <5KDa

[0070] According to the extraction parameters optimized by the orthogonal test in Example 1, 300 mg of the Periplaneta americana acetone-water extract prepared in 2.1 was dissolved in 1000 milliliters of warm water; In an ultrafiltration device with a 5KD...

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Abstract

The present invention relates to preparation of a blattodea polypeptide, and uses of the blattodea polypeptide in anti-gram-positive bacteria and anti-gram-negative bacteria. Specifically the present invention relates to a blattodea extract effective fraction having a function of prevention and treatment of bacterial infection diseases, a preparation method and a medical use thereof. The Periplaneta Americana extract effective fraction of the present invention is a polypeptide substance with a molecular weight less than 5000 dalton, and is prepared by the following steps: carrying out solvent extraction and membrane separation on fresh polypide or dried polypide of Periplaneta Americana to obtain the product of the present invention, wherein the polypeptide active substance of the effective fraction has significant anti-gram-positive bacteria activity and anti-gram-negative bacteria activity, can be prepared into forms of a hydrogel, a cataplasm, lyophilized powder, a water agent, an aerosol, a suppository, a film agent, an external application liniment, and an ointment, and can be used for preparations of drugs for prevention and treatment of diseases related to gram-positive bacteria and gram-negative bacteria and infections, daily chemical products or medical devices.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular, the invention relates to an effective part of a cockroach extract capable of preventing and treating bacterial infectious diseases, a preparation method and a medical application thereof. The effective part polypeptide active substances can be prepared into forms such as hydrogel, cataplasm, freeze-dried powder, water, aerosol, suppository, film, external liniment, ointment, etc., for the preparation of treatment and prevention of Gram Drugs, daily chemical products or medical devices for diseases related to Gram-positive and Gram-negative bacteria infections. Background technique [0002] Bacteria are abundant in nature. Staphylococcus aureus (Staphylococcus aureus, referred to as "Staphylococcus aureus") is a Gram-positive coccus, which is the most common pathogenic bacteria that has a great impact on human health. The main infections caused by humans are boils, pus Blisters, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61K35/64A61K8/64A61P31/04A61Q19/10A61Q19/00A61L15/44A23K1/16A01N63/02A01P1/00
CPCY02A50/30
Inventor 钱金栿杨荣新高鹏飞巫秀美赵昱
Owner DALI UNIV
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