104 results about "Periplaneta" patented technology

An extract of American periplaneta and its medicinal composition with high content of general amino acids are disclosed. Its preparing process includes such steps as immersing American periplaneta in petroether for removing fat, extracting in water or alcohol, and mixing with pharmacologically acceptable additives.

The medicinal American giant roach extractum preparing process includes alcohol extraction process and water extraction process. The alcohol extraction process includes hot water killing of roach, drying, crushing, sieving; alcohol soaking for 40-50 hr; alcohol reflux extraction in a multifunctional extractor; recovering alcohol; recovering medicine liquid via oil-water separation; and filtering and concentrating medicine liquid to obtain extractum. The water extraction process includes extraction of the material after recovering alcohol in alcohol extraction process in distilled water; recovering medicine liquid and oil-water separation; filtering and concentrating medicinal liquid; and cooling to obtain extractum. The said preparing process has high yield, and the product has total amino acid content up to 50.4 %.

The present invention relates to Periplaneta Americana or its ethanol extract for the treatment of inflammation. Specifically, said inflammation includes cervical erosion, ulcerative colitis, skin ulcer, mucosal ulcer and postoperative condyloma acuminatum. Treating said disorders by Periplaneta Americana or its ethanol extract has the advantages of effectively reducing bleeding, shortering healing time, significant curative effect, no adverse reactions and low cost. Periplaneta Americana or its ethanol extract provides a new selection for the clinical treatment of cervical erosion, ulcerative colitis and postoperative condyloma acuminatum.

The invention discloses an artificial propagation method for periplaneta americana. The method comprises the following steps: a sack, an incubator, a breeder box and an egg tray are sealed and fumigated for one hour after being evenly mixed with disinfecting solution; the temperature in the incubator is controlled between 29 DEG C and 33 DEG C through an automatic temperature and humidity control device, the relative humidity is controlled between 80 percent and 85 percent, and the inside of the incubator is well ventilated; a layer of sack is arranged at the bottom part of the breeder box or a hatching bamboo sieve, a layer of ootheca with a thickness of 1 cm to 3 cm is placed on the sack, a layer of egg tray is transversely placed on the ootheca, and a layer of sack is also covered on the egg tray; the breeder box or the hatching bamboo sieve is placed on a partition board with a hole in the incubator or on a hatchery rack of a hatching house; and when 10 to 30 larvae are generated in each egg tray, the egg trays in which the larvae are hidden are gently transferred to breeding chips, and the larvae hidden in the egg trays naturally enter in the chips to grow. The method can overcome the problems of low hatchability and long hatching period, and is suitable for the large-scale artificial propagation of the periplaneta americana.

The medicinal American giant roach extractum preparing process includes alcohol extraction process and water extraction process. The alcohol extraction process includes hot water killing of roach, drying, crushing, sieving; alcohol soaking for 40-50 hr; alcohol reflux extraction in a multifunctional extractor; recovering alcohol; recovering medicine liquid via oil-water separation; and filtering and concentrating medicine liquid to obtain extractum. The water extraction process includes extraction of the material after recovering alcohol in alcohol extraction process in distilled water; recovering medicine liquid and oil-water separation; filtering and concentrating medicinal liquid; and cooling to obtain extractum. The said preparing process has high yield, and the product has total amino acid content up to 50.4 %.

The invention relates to a periplaneta americana medicinal material fingerprint quality determination method. The fingerprint obtained by the periplaneta americana medicinal material fingerprint quality determination method has a good chromatographic peak resolution degree. The determination method has reasonable detection time and good repeatability and stability. Compared with the existing reported periplaneta americana medicinal material fingerprint technology, the method improves a periplaneta americana medicinal material quality control level. The fingerprint technology effectively evaluates and controls periplaneta americana medicinal material quality, guarantees periplaneta americana extract quality stability under a certain preparation condition, further effectively controls quality of periplaneta americana extract-containing preparations such as Kangfuxin tablets, Kangfuxin capsules, a Kangfuxin liquid, Xinmailong, Ganlong capsules and the like.

The invention discloses a quality control method of Periplaneta americana freeze-dried powder. The quality control method comprises the steps of carrying out amino acid identification by virtue of a thin layer chromatography, carrying out content determination on amino acid and polypeptide by virtue of ultraviolet-visible spectrophotometry, and carrying out content determination on uracil, hypoxanthine and creatinine by virtue of a high performance liquid chromatography. According to the quality control method, the quality of the Periplaneta americana freeze-dried powder is detected and controlled from several aspects of the qualitative identification of amino acid, the content determination of amino acid, polypeptide and nucleoside components, the composition analysis of amino acid and the molecular weight determination of polypeptide. Powerful guarantee is provided for the quality of the Periplaneta americana freeze-dried powder, and the quality and the treatment effect of a later-period preparation taking the Periplaneta americana freeze-dried powder as an intermediate is guaranteed.

The invention relates to a multi-PCR primer and a method for quickly identifying a blattaria insect variety, belonging to the technical field of applied biology. The multi-PCR primer for quickly identifying the blattaria insect variety comprise a periplaneta americana primer, a periplaneta fuliginosa primer, a periplaneta australasiae primer, a periplaneta brunnea primer, a blattella germanica primer and a ground beetle primer. The identifying method comprises the following steps: extracting blattaria insect genome total DNA, performing multi-PCR amplification, and judging a variety of a blattaria insect to be detected according to PCR product size obtained by sepharose gel electrophoresis detection. PCR product strip sizes of periplaneta americana, periplaneta fuliginosa, periplaneta australasiae, periplaneta brunnea, blattella germanica and ground beetle are respectively 452bp, 125bp, 160bp, 284bp, 253bp and 380bp. The identifying primer and the multi-PCR method can be used for sensitively and quickly determining the variety of the blattaria insect sample to be detected, and the method has the advantages of simple operation, high repeatability, low cost and the like in comparisonto a traditional detection method.