61 results about "Insect sample" patented technology

The invention discloses a preparing method of insect sample, which comprises the following steps: fixing living insect, dehydrating through alcohol, replacing, permeating, solidifying or polymerizing to obtain the insect sample, fitting for studying microscopic structure and super-microscopic structure of insect cell.

The invention discloses a making method of an artificial cantharias specimen which is made by taking urea-formaldehyde resin as a main raw material and sodium hydroxide and glacial acetic acid as auxiliary raw materials. The making method comprises the following steps of: synthesizing a urea-formaldehyde resin raw monomer, pre-treating insects, preparing a ripe monomer, preparing a mould, fixing and embedding an insect sample, demoulding and polishing, wherein the raw monomer is formed by adding sodium hydroxide and glacial acetic acid into formaldehyde and urea as raw materials and then polymerizing, and the proportion of the formaldehyde to the urea is equal to 55:18 to 50:22; the urea-formaldehyde resin has the shrinking percentage of 5-20%, the hardening time of 2-5d and the bubbling number of 0-5; and the ripe monomer is prepared by mixing the liquid urea-formaldehyde glue of the raw monomer and an extension agent, adding glacial acetic acid and stirring, wherein the extension agent is selected from polyethylene glycol 600, DEHP (Di 2-Ethyl Hexyl Phthalate) and DINP (Diisononyl Phthalate). The making method disclosed by the invention is simple and rapid in process, low in cost and the raw materials are easy to purchase; and a sample is high in strength and good in toughness, glossiness and transparency.

The invention discloses a Conopomorpha sinensis Bradley forecasting method and solves the problems of the prior art that the forecasting time span is short and insect samples are hard to collect. The Conopomorpha sinensis Bradley forecasting method includes studying larva ages, maturing standard and developmental duration of the larva at different ages under different temperatures; calculating suitable control data for the Conopomorpha sinensis Bradley from the larva stage. In this way, the forecasting time span is expanded, the conventional method that the suitable control data only can be calculated from pupa stage is changed, the insect samples are easy to collect, and forecasting efficiency is greatly improved.

The invention discloses an insect quantity survey method which comprises the following steps: (1) beating a branch carrying insects to ensure that the insects drop into a collecting container arranged below the branch and provided with a piece of wet paper, thereby obtaining a piece of wet paper carrying the insects; (2) placing the wet paper carrying the insects in an insect collecting bag, and sealing the insect collecting bag to obtain a collecting bag filled with the insects, wherein the insect collecting bag contains a substance capable of killing or narcotizing insects; (3) calculating the quantity of the insects in the collecting bag filled with the insects, thereby achieving insect quantity detection. Proved by tests, the insect quantity survey method is accurate and reliable in data, simple and convenient to operate, and low in production cost, and facilitates further classification, identification, number representation, and preparation of insect specimens.

The invention discloses a ground surface insect separation device which comprises a collection section and a placement section, wherein a channel for passing of ground surface insects is arranged between the placement section and the collection section, and the channel comprises a transition section which is horizontally arranged and has a rough surface inside, a smooth section which is verticallyarranged and has a smooth surface inside, and a bracket which is locked tightly and can adjust the height the transition section, wherein an inlet of the smooth section is communicated to an outlet of the transition section; the placement section is provided with a storage container used for containing a plurality of ground surface insects and sundries, the bottom of the storage container is provided with a hood used for covering the ground surface insects and sundries, and the top of the storage container is provided with an inlet connected with the inlet of the transition section; the collection section is provided with a collection container for containing the plurality of ground surface insects, and the top of the collection container is provided with a narrow opening, and the narrowopening is communicated with an outlet of the smooth section. The ground surface insect separation device not only avoids the injury to human bodies caused by toxic and harmful substances, also avoiddamaging insect samples, and the application range is not limited.

The invention provides a device for measuring the walking and creeping characteristics of an insect on three planes. The device is characterized by comprising a creeping drum, a vibration force measuring mechanism, a three-position creeping force measuring mechanism, a three-position fixing base, a side-looking camera and a sound pick-up, wherein the creeping drum is used for accommodating the insect; the vibration force measuring mechanism is used for testing the sole creeping frequency of the insect; the side-looking camera is used for viewing the creeping posture of the insect; the sound pick-up is arranged in the creeping drum, and is connected with vibration force measuring mechanism; and the three-position creeping force measuring mechanism is arranged on the three-position fixing base. The device has the advantages and positive effects that during testing, treatment on a living insect sample is simple, the contact force and contact state of the sole of the insect with the creeping drum can be tested more accurately, the creeping force value of the insect at a flat position, a suspended position and a side position can be tested conveniently, and the range of measuring objects is wider.

The invention relates to a multi-PCR primer and a method for quickly identifying a blattaria insect variety, belonging to the technical field of applied biology. The multi-PCR primer for quickly identifying the blattaria insect variety comprise a periplaneta americana primer, a periplaneta fuliginosa primer, a periplaneta australasiae primer, a periplaneta brunnea primer, a blattella germanica primer and a ground beetle primer. The identifying method comprises the following steps: extracting blattaria insect genome total DNA, performing multi-PCR amplification, and judging a variety of a blattaria insect to be detected according to PCR product size obtained by sepharose gel electrophoresis detection. PCR product strip sizes of periplaneta americana, periplaneta fuliginosa, periplaneta australasiae, periplaneta brunnea, blattella germanica and ground beetle are respectively 452bp, 125bp, 160bp, 284bp, 253bp and 380bp. The identifying primer and the multi-PCR method can be used for sensitively and quickly determining the variety of the blattaria insect sample to be detected, and the method has the advantages of simple operation, high repeatability, low cost and the like in comparisonto a traditional detection method.

The invention relates to an air blowing type insect sucking device. Insect storage chambers of the insect sucking device comprise a front insect storage chamber and a back insect storage chamber, the outer diameter of one end of the front insect storage chamber is in interference fit connection with the inner diameter of one end of the back insect storage chamber, the other end of the front insect storage chamber is connected with a suction head, and the other end of the back insect storage chamber is connected with a rubber air blower. In the use process, a proper suction head is selected according to the size of insects, the air blower is pinched by the hand, and then, the air blower is fast released, so that the negative pressure is formed inside the air blower, and insect samples are sucked into the insect sucking device, and are blocked on a filter membrane. The suction head and the air blower are pulled down, an upper sealing head is covered, a label is pasted, and the mixed containing of different kinds of insects collected in different time can be avoided. During the sample encapsulation, the suction head can directly extend into a poison bottle for killing the insects. The air blowing type insect sucking device has the advantages that the structure is simple, the operation is convenient, the collection is fast, the direct batch storage can be realized, the recording is accurate, and the like.

The invention discloses an insect specimen display plate processing production line, which comprises a work table and a conveying belt, wherein the conveying belt is arranged at the front end of the work table and is used for conveying insect samples; a cavity structure is formed inside the work table; one end of the work table is provided with a conveying unit; a carrying plate is arranged above the other end of the work table; a cutting unit is arranged on the carrying plate; a sample installing hole punching unit and a lettering unit are sequentially arranged between the conveying unit and the cutting unit; a controller is arranged on the work table, and is electrically connected with the conveying belt, the conveying unit, the cutting unit, the lettering unit, a No.1 air cylinder, a No.1 rotating motor and a punching head. The insect specimen display plate processing production line has the beneficial effects that the structure is simple; the practicability is high.

The invention discloses a wheat yellow striate virus N gene recombination expression protein, a method for preparing polyclonal antibodies and application thereof. The method for preparing the polyclonal antibodies includes steps of synthesizing genes by optimization codons according to sequences of N genes of WYSV (wheat yellow striate viruses) for the first time and subcloning the genes into target vectors pET-30a for expressing Escherichia coli; carrying out prokaryotic expression on recombinant proteins and immunizing New Zealand white rabbits to obtain the WYSV-N protein polyclonal antibodies. The wheat yellow striate virus N gene recombination expression protein, the method and the application have the advantages that as shown by Western blot detection, the antibodies prepared by theaid of the method can be specifically bound with toxic insect samples, and accordingly the high specificity of the obtained antibodies is illustrated; whether vector insects carry the WYSV or not canbe quickly detected by the aid of immunofluorescence detection processes under laboratory conditions, and accordingly a foundation can be laid for research on WYSV prediction and forecast and psammotettix striatus L. and WYSV interaction mechanisms.

The invention discloses a barn worm gas and water detection unit with a self protection function. The barn worm gas and water detection unit comprises a control circuit board, a gas collection device,an air path control device, a detection element, an electromagnetic valve control circuit and a pump control circuit. The goal of counting pest attraction density, shooting pest images, and detectingthe phosphoretted hydrogen concentration, carbon dioxide concentration, oxygen concentration and grain pile moisture content of the grain pile can be realized through extracting insect samples and gas samples in a grain pile. The invention also discloses a detection method of the barn worm gas and water detection unit with the self protection function. During the application, the detection flow process can be conveniently controlled; the damage to an oxygen sensor, a carbon dioxide sensor and a temperature and humidity sensor by phosphoretted hydrogen gas can be prevented; the requirements ofthe intelligent warehouse storage can also be met.

Devices and methods for insect surveillance are disclosed. In particular, the invention relates to devices for collecting samples of salivary fluid from insects and multiplexed high-throughput methods of screening insect samples for specific insect species, genetically modified strains, and insect-borne pathogens. An attractant is used to induce insects to deposit salivary droplets in a 2-dimensional microfluidic array having media-filled pockets sized to collect saliva from a single insect. The array for sample collection can be integrated with a microfluidic device for high-throughput processing of insect samples. The microfluidic device can be designed to perform multiplexed PCR or immunoassays, for example, for insect genotyping or pathogen detection. Devices can be used for screening populations of insects for arthropod-borne diseases, studying genetically modified populations released into the wild, determining the presence and quantity of specific arthropod species and pathogens, and delivery of bioagents.

The invention relates to a photographic device used for photography of inset specimens and a photographic method thereof and belongs to the technical field of special operation devices for photography. The photographic device includes a connecting rod, a camera and a supporting rod; a connecting buckle is arranged on the connecting rod, one end of the connecting buckle sleeves the connecting rod and moves up and down along the connecting rod, and the other end of the connecting buckle is used for installing the camera to achieve fixation of the position of the camera; the supporting rod is positioned below the camera, a sample pile is arranged on the supporting rod and used for placing a sample to be photographed, and the spacing between the sample to be photographed and a lens of the camera is 15-50 cm. The photographic device is applied to photography of specimens or samples of animals and plants such as insects and butterflies and has the advantages of being simple, effective, clearin photography, clean in background and the like.

The invention belongs to the technical field of specimen making, and discloses an insect specimen making method, which comprises the following steps: stretching crimped whiskers, feet, antennae and the like from a collected or softened specimen in a softener by using a teasing needle, separately pressing down and resetting two wings, fixing an insertion needle, and making an insect specimen by using a wing-unspreading five-needle method and a wing-spreading five-needle method. The five-needle method provided by the invention mainly solves the problem that various large-batch insects such as butterfly, moth, bee, fly, cicada, dragonfly and the like need a specimen making technology for wing spreading, making, observing and researching characteristics, and has higher efficiency than the traditional groove and groove type wing spreading plate wing spreading making method with slower duty. By adopting the five-needle method, the insect specimen making efficiency can be greatly, quickly andwell improved according to local conditions.

The invention discloses a field multifunctional insect detaining and collecting test tube device. One end of an insect detaining tube is open, the other end of the insect detaining tube and the top end of an insect storage tube are in threaded connection, and the bottom end of the insect storage tube, a ventilating screen and a generator are in threaded connection. An opening and closing baffle is arranged in the insect detaining tube, the ventilating screen is arranged at the joint of the insect storage tube and the generator, a ventilating screen hole plate is arranged on the ventilating screen, small screen holes are formed in the plate, and cotton is placed in the generator. The device has the advantages that all the parts are convenient to detach and replace; re-escape of insects is effectively avoided; drop resistance is achieved, and it is avoided that the appearance integrity of small insects is damaged due to the fact that plastic materials generate static; the insects in the tube can be paralyzed through alcohol gas to be in a coma, the active degree of the insects is reduced, limb damage and deficiency caused by space competition fight among the insects are reduced, DNA of insect specimens saved after alcohol fumigation is not prone to degradation, molecular relevant experiments can be carried out, and meanwhile later insect shape classification work is also promoted.

The invention relates to a method for measuring the content of in vivo biotin of an insect. The method comprises the following steps: (1) extracting an insect sample; (2) preparing a biotin gradient solution; (3) preparing a biotin assay culture solution; and (4) determining biotin. The lactobacillus plantarum, various culture media and the like used in the detection method are common strains or consumables in the market, the cost is low, the biotin detection cost is remarkably reduced, and the culture method is simple; the detection time of the method is 22 hours and is reduced by 22 to 26 hours compared with that of a commercial kit, and the efficiency of insect vitamin detection is improved.

The invention relates to a touch-killing site experiment evaluating tool which comprises a storage container for storing insect samples, a cover type device detachably connected with the storage container, and a collecting container arranged at the end, away from the storage container, of the cover type device and detachably connected with the cover type device. The storage container, the cover type device and the collecting container are communicated. A piston pusher for pushing the insect samples into the cover type device and the collecting container is arranged in the storage container. The touch-killing site experiment evaluating tool can scientifically, effectively and accurately evaluate the insecticide staying and spraying effect and is convenient to clean and high in applicability. According to the touch-killing site experiment evaluating method, by means of the touch-killing site experiment evaluating tool, the staying and spraying effect of an insecticide can be rapidly andaccurately evaluated.

The invention discloses a method for detecting insect in-vivo juvenile hormone JH II based on chromatography-mass spectrometry and relates to application of analytical chemistry in entomology research. The method comprises the specific steps that an insect sample is taken, grinding balls, acetonitrile, a sodium chloride water solution and normal hexane are added for grinding, high-speed centrifugation is performed to obtain a normal-hexane-layer crude extract, then normal hexane is added to the sample for two times of grinding and extraction, the crude extract obtained through three times of grinding is added to a collecting bottle, and nitrogen blow-drying is performed; re-dissolution is performed with an acetonitrile-water solution, then the acetonitrile is eluted for three times by using a solid-phase extraction column, and eluant in a collecting box is taken out and subjected to nitrogen blow-drying; re-dissolution is performed with an acetonitrile-water solution, and analysis is performed by using a liquid chromatograph-mass spectrometer. The detecting method is high in sensitivity, strong in matrix interference resisting capability and good in repeatability and can provide help for research of in-vivo juvenile hormone JH II change laws of insects at all stages.

The invention discloses a cicada sample storage device. The cicada sample storage device comprises a supporting mechanism and a display mechanism. The supporting mechanism comprises a base, a supporting frame, a shaft sleeve bearing, a universal ball and an annular air bag with a gas inlet and outlet. The supporting frame is a cylindrical part made of a transparent material, the display mechanismcomprises a transparent cylinder, a partition plate, a cover plate and a bottom plate, the annular air bag is arranged in a middle hole of the shaft sleeve bearing and is fixedly connected with an inner ring of the shaft sleeve bearing, the shaft sleeve bearing is arranged over the supporting frame, an outer ring of the shaft sleeve bearing is fixedly connected with the upper end of the supportingframe, the lower end of the supporting frame is fixedly connected with an upper plate face of the base, the universal ball is embedded on an upper plate face of the base, the top end of a ball body of the universal ball is located above the upper plate face of the base, and a vertical ball axis of the ball body of the universal ball coincides with the middle axis of the shaft sleeve bearing. An insect sample can be circumferentially observed in a full-angle manner, contact with the sample is avoided when the sample is subjected to multi-angle observing, the damage to the sample can be reduced, and dismounting and mounting of the display mechanism and the supporting mechanism can be facilitated.

The invention provides a migratory insect yelk collagen protein detection test paper based on colloidal gold chromatography and a preparation method thereof. The test paper is characterized in that a nitrocellulose coating film overlapped with a gold-labelled combined pad, a sample pad and an absorbent pad is arranged on a PVC (polyvinyl chloride) plastic bottom plate; The nitrocellulose coating film is provided with a detection line coated with a polyclonal antibody and a quality control line coated with a goat anti mouse IgG; and the gold-labelled combined pad is coated with a colloidal gold grain labelled antibody. The preparation method comprises the following steps: firstly preparing a monoclonal antibody, the polyclonal antibody and the goat anti mouse IgG and utilizing the monoclonal antibody to prepare the colloidal gold grain labelled antibody; then sequentially overlapping and pasting the sample pad, the gold-labelled pad, the nitrocellulose coating film and the absorbent pad on the PVC plastic bottom plate, wherein the nitrocellulose coating film is provided with the detection line and the quality control line; and coating the detection line with the polyclonal antibody, coating the quality control line with the goat anti mouse IgG and coating the gold-labelled combined pad with the colloidal gold grain labelled antibody, thus obtaining the detection test paper. The test paper provided by the invention is convenient and efficient and can be used for semi-quantitatively detecting the content of the yelk collagen protein in a migratory insect sample within 5-10 minutes so as to determine the source property of the migratory insect.

The embodiment of the invention discloses a multifunctional insect specimen collecting device suitable for a wild environment. The device comprises an upper bottle cover, a bottle body and a lower bottle body, wherein the bottle body comprises an inner cavity; the inner cavity is divided into m isolated isolation cabins; the upper bottle cover comprises an inner cover and an outer cover, the innercover is fixed to the bottle body, an inner hole matched with each isolation cabin in position is formed in the inner cover, and the openings of the inner holes are uniformly distributed in the circumferential direction of the inner cover; the outer cover is connected to the outer side of the inner cover in a sleeving manner and can rotate relative to the inner cover, and an outer hole is formedin the outer cover; the lower bottle body for collecting and storing insect specimens in triangular paper is arranged at the bottom of the bottle body; inner transverse plates are arranged in one or more isolation cabins of the bottle body, the inner transverse plates are arranged in the isolation cabins and divide the isolation cabins into upper and lower parts isolated from each other, and volatile liquid is arranged in each lower part; and a ventilating liquid isolating hole is formed in each inner transverse plate, and a cotton roll is arranged on each inner transverse plate.

The invention relates to the technical field of specimens, and discloses a storage method of insect specimens, which comprises the following steps: adding an antibacterial composite pad with bacteriostatic action and drying and moisture-absorbing action into an insect specimen box, boxing the insect specimens according to normal steps, and then packaging the box in vacuum by using a transparent sealing bag. And insect specimens are protected as much as possible. According to the method, the food preservation technology is creatively applied to preservation of the insect specimens, the method has extremely high application value in storage and transportation of the insect specimens, and the preservation performance of the method is also very excellent through vacuum sealing preservation.

The invention discloses an insect specimen 3D shooting table which comprises a rotating platform device and a shooting platform device which are both arranged on a lifting table. The rotating platform device comprises a motor, a rotating table, a U-shaped arm, a rotator and a specimen objective table; the rotating table is connected with the motor and provided with a U-shaped arm, a specimen objective table is rotationally connected to the upper portion of the U-shaped arm, a rotator is installed on one side of the U-shaped arm, and the rotator is connected with the specimen objective table; the shooting platform device comprises a left-right guide rail, a front-back guide rail, a motor, a holder and a digital camera shooting table. The left-right guide rail is slidably connected with a front-back guide rail, the front-back guide rail is provided with a motor, the motor is connected with a holder, and a digital camera is fixed on the holder. During shooting, the angle and the direction of the specimen objective table can be changed through the motor, so that 3D shooting of insect specimens is achieved, and the device is convenient to operate, high in automation degree, capable of being manually and electrically operated and high in practicability.

The invention relates to the technical field of specimens, in particular to a vacuum storage method for insect specimens. According to the vacuum storage method, non-woven fabric adsorbed with an antibacterial agent is added into an insect specimen box to achieve the bacteriostatic effect, modified hydrogel with a humidifying function is heat-sealed in the non-woven fabric, and then the insect specimens are boxed. According to the vacuum storage method, a vacuum sealing technology is applied to preservation of the insect specimens, the method has extremely high application value in transportation and storage of the insect specimens, and the preservation performance of the insect specimens is very excellent when the insect specimens are subjected to vacuum sealing preservation.

The invention relates to a simplified anatomical model establishment method for insect dose rate estimation, which belongs to the technical field of non-human species evaluation, and comprises the following steps: dissecting an insect sample to obtain each tissue / organ sample, and measuring appearance parameters and quality of each part of sample; detecting the density and the total element content of each part of sample; geometric description is carried out on each tissue / organ, a curved surface related to each part is determined, and a curved surface equation and a composition relation between the curved surfaces are given; establishing a Monte Carlo simplified anatomical model of the insect according to the curved surfaces determined in the step S3 and the curved surface composition relation of the parts; and estimating the insect dose rate by using the Monte Carlo simplified anatomical model of the insect. According to the method provided by the invention, a model which is closer to a real situation and is used for distinguishing tissues / organs can be provided for radiation influence evaluation of insects, and excessive protection in nuclear industry environment evaluation caused by conservative estimation is avoided.

The invention discloses an extracting agent for DNA extraction and application thereof. The extracting agent for DNA extraction is composed of Tris-base, NaCl, PVP and Tween-20, and can rapidly extract a trace amount of DNA from insect materials, and only the extracting agent, instead of other liquid, needs to be disposed in the sample extraction process. The reagents adopted by the extracting agent are all commonly used chemical reagents in a laboratory, and are low in cost, so that the cost is reduced; and the extracting agent is stable in component, does not need to be prepared when needed,can be repeatedly used, and can be stored at room temperature for at least 3 months. At the same time, the process of extracting the trace amount of DNA of insect samples is simple and rapid, and theextraction can be completed within 30 s. The DNA extracted by the extracting agent has stable quality and can be directly used for subsequent experimental detection. The biggest advantage of the method is that only one extracting agent is used, the extraction process can be completed within 30 s, and the extracted DNA has stable quality and can be directly applied to subsequent experimental detection.

The invention discloses a remote intelligent identifying, counting and collecting insect specimen operation platform system, and belongs to the technical field of remote transmission insect conditionpredicting instruments. The following scheme is put forwards now, the remote intelligent identifying, counting and collecting insect specimen operation platform system comprises a collecting box; a circular opening part is formed in the top of the collecting box, and a rotating column is rotationally installed in the circular opening part of the collecting box; insect falling rotating platform motors are installed on the rotating column; a left insect falling rotating platform and a right insect falling rotating platform are fixed to the left side and the right side of the upper portion of therotating column correspondingly; and an insect body specimen recycling opening is formed by the rear portions of the left insect falling rotating platform and the right insect falling rotating platform and the rear portion of the circular opening part. The front portions of the left insect falling rotating platform and the right insect falling rotating platform and the front portion of the circular opening part form an insect falling rotating platform positioning fracture; an insect body shooting system is installed on the upper portion of the left side of the rotating column; and an insect falling rotating platform cleaning brush is installed above the insect body specimen recycling opening in a matched mode. The system is ingenious in design, reasonable in structure, accurate in identifying and counting and suitable for using and popularizing.

The present invention discloses an electric shock pest killing device for an automatic crop pest monitoring and reporting system. The electric shock pest killing device comprises a trapping lamp, an insulating platform, a pest receiving platform and a plurality of electric pole groups; each electric pole group is composed of two direct-current electric poles as a positive electrode and a negativeelectrode, and the plurality of electric pole groups are vertically arranged around the trapping lamp; the trapping lamp penetrates through the insulating platform and is fixed to the top of the monitoring and reporting system, each direct-current electric pole is vertically fixed on the insulating platform, and a gap is reserved between the bottom of each direct-current electric pole and the pestreceiving platform; and the insulating platform is provided with an eccentric shaft motor, so that the insulating platform can drive all the electric poles to vibrate leftwards and rightwards simultaneously. While automatic identification of insect sample image acquisition conditions by crop pests is facilitated, equipment is simplified and the energy consumption is reduced, so that the automaticcrop pest monitoring and reporting system can be popularized in a large area by utilizing solar energy or wind energy.