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Migratory insect yelk protein detection test paper based on colloidal gold chromatography and preparation method thereof

A vitellogenin and detection test paper technology is applied in the field of colloidal gold chromatography method for migratory insect vitellogenin detection test paper and its preparation, and can solve the problems that hinder the accuracy rate of rice leafroller forecasting, obvious human factors, and workload. Large and other problems, to achieve the effect of easy portability, high detection accuracy, and strong specificity

Inactive Publication Date: 2012-04-18
HUAZHONG AGRI UNIV
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Problems solved by technology

However, there are defects such as heavy workload, low accuracy, obvious human factors, and the need for professional equipment in the actual operation of dissecting the ovary, which hinders the accuracy of the rice leaf roller. It is necessary to develop a convenient, fast and specific method. Forecasting method

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  • Migratory insect yelk protein detection test paper based on colloidal gold chromatography and preparation method thereof
  • Migratory insect yelk protein detection test paper based on colloidal gold chromatography and preparation method thereof

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Embodiment Construction

[0025] The applicant has found through research that the ovary development of the rice leaf roller adult is closely related to vitellogenesis, and the continuous deposition of yolk (mainly vitellogenin) in the ovariole promotes the development and maturation of the ovary, so it can be achieved through the deposition of vitellogenin. To judge the progress of ovarian development. Based on this idea, the applicant prepared an antibody to vitellogenin in the rice leaf roller and developed a portable colloidal gold test strip to detect the content of vitellogenin in the ovary and quickly judge the ovarian development of the rice leaf roller progress, determine the nature of the insect source, and provide a new method for the accurate forecasting of the rice leaf roller.

[0026] The detection test strip of the present invention is that a nitrocellulose coating film 3 is placed in the middle of the PVC plastic base plate 1, and the left side of the nitrocellulose coating film is suc...

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Abstract

The invention provides a migratory insect yelk collagen protein detection test paper based on colloidal gold chromatography and a preparation method thereof. The test paper is characterized in that a nitrocellulose coating film overlapped with a gold-labelled combined pad, a sample pad and an absorbent pad is arranged on a PVC (polyvinyl chloride) plastic bottom plate; The nitrocellulose coating film is provided with a detection line coated with a polyclonal antibody and a quality control line coated with a goat anti mouse IgG; and the gold-labelled combined pad is coated with a colloidal gold grain labelled antibody. The preparation method comprises the following steps: firstly preparing a monoclonal antibody, the polyclonal antibody and the goat anti mouse IgG and utilizing the monoclonal antibody to prepare the colloidal gold grain labelled antibody; then sequentially overlapping and pasting the sample pad, the gold-labelled pad, the nitrocellulose coating film and the absorbent pad on the PVC plastic bottom plate, wherein the nitrocellulose coating film is provided with the detection line and the quality control line; and coating the detection line with the polyclonal antibody, coating the quality control line with the goat anti mouse IgG and coating the gold-labelled combined pad with the colloidal gold grain labelled antibody, thus obtaining the detection test paper. The test paper provided by the invention is convenient and efficient and can be used for semi-quantitatively detecting the content of the yelk collagen protein in a migratory insect sample within 5-10 minutes so as to determine the source property of the migratory insect.

Description

technical field [0001] The invention relates to a test paper for detecting vitellogenin (Vg) of migratory insects by colloidal gold chromatography and a preparation method thereof. Background technique [0002] Rice leaf roller (Cnaphalocrocis medinalis Guenée) is an important migratory pest widely distributed in Asia and East Africa. It is distributed in almost all rice producing areas in my country, but there are obvious regional differences in the degree of occurrence. The hazard area is south of the line from Taiyi Mountain to Qinling Mountains. Adult rice leaf rollers have long-distance migratory habits, and there are 5 northward migrations and 3 southward migrations in my country every year. The large-scale migration makes the harm of the rice leaf roller sudden, and the prediction is extremely difficult, and it is often caught off guard and causes heavy losses. Therefore, it is very important to predict it accurately, timely and accurately. [0003] Forecasting and f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/558G01N33/531
Inventor 牛长缨顾永征翟保平林拥军
Owner HUAZHONG AGRI UNIV
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