The invention relates to a sterilization and fresh-keeping agent containing the plant vinegar fluid and the application thereof, belonging to the sterilization and fresh-keeping agent technical field, which is characterized in that the sterilization and fresh-keeping agent comprises from 50% to 80% of plant vinegar fluid, from 10% to 19% of low residue germicide, from 4% to 12% of sticking agent, from 3% to 15% of mould proofing antisepsis color fixative, and from 3% to 6% of potentiating agent. The sterilization and fresh-keeping agent has the advantages of utilizing the purely natural and strong anti-pest activity vinegar as the active component, compositing the minor chemical agent, having functions of fresh-keeping, sterilization, color fixing, and strong inhibition to the bacteria, mould, and yeast, enabling to prevent the pathogeny objects from ingression, lowering the water in the fruit and vegetable to evaporate and avoiding to shrink, blocking the gas exchange of the fruit and the vegetable, decreasing the breathing intensity, restraining the activity of the enzyme inside the fruit and the vegetable, decelerating the lowering speed of the sugar acid content, extending the storage life of the fruit and vegetable. The processing method for the sterilization and fresh-keeping agent has the advantages of simple process, less equipment investment, rich material resource, higher yielding rate, energy saving, safe storage and transportation, and low production cost.