53 results about "Commercial kit" patented technology

An outdoor activity glove is detailed that includes a hand covering defining a volume adapted to receive a human hand therein. The instrument attachment module is secured to the exterior surface of the hand covering by way of a fastener. The instrument attachment module includes a side view mirror and at least one additional accessory of a clock, a compass, a light and a thermometer. A commercial kit is intended to retrofit a conventional hand covering. The commercial kit includes an instrument attachment module including a side view mirror and at least one recess adapted to receive an accessory such as a clock, compass, storage compartment, light, or a thermometer. A fastener is provided for securing the instrument attachment module to the exterior surface of a conventional hand covering.

The invention provides a fluorescence-labeled composite amplification kit for Y chromosome STR (short tandem repeat) gene loci capable of improving distinguishing capability. When the kit is used for detecting DNA (deoxyribonucleic acid) gene, not only can 17 STR gene loci of DYS391, DYS389I/II, DYS439, DYS438, DYS456, DYS458, DYS437, DYS635, DYS448, Y-GATA-H4, DYS19, DYS392, DYS393, DYS390 and DYS385a/b, which can be analyzed by the commercial kit, be amplified and analyzed, but also at least one of STR gene loci of DYS449, DYS527a/b, DYS522, DYS388, DYS447 and DYS444 can be simultaneously amplified and analyzed, so that the accumulative individual distinguishing capability and cumulative probability of exclusion of the system are improved, and the individual distinguishing capability is improved overall.

The invention discloses a soluble expression method of a recombinantpeste des petits ruminant virus H-F fusion protein. The solubleexpressionmethod comprises a step of expressing an encoding gene of a protein in a living thing, thereby obtaining the protein, wherein the living thing is a microorganism, a plant or a non-human animal; the protein is a protein of a) or b), namely a) a protein consisting of amino acid sequences of SEQ ID No.2, and b) a soluble protein generated by substituting and/or deleting and/or adding one or more amino acid residues into an amino acid sequence of SEQ ID No.2. The recombinantpeste des petits ruminant virus H-F fusion protein treated with the solubleexpression method of the recombinantpeste des petits ruminant virus H-F fusion protein disclosed by the invention is high in expression level and low in production cost, and a basis is made for furtherdevelopment of commercial kits.

The invention discloses a foot-and-mouth disease virus (FMDV) 2C3ABC recombinant protein as well as a preparation method and application thereof, and belongs to the field of pharmaceutical biotechnology. According to the invention, the mutation of the following sites is performed on the basis of an original FMDV 3C protease gene: Cys142-Ser, Cys163-Gly. FMDV recombinant protein 2C3ABC is expressed as an inclusion body in the bacterial cytoplasm, and is subjected to separation, denaturation, renaturation and multi-step purification, to obtain a complete and enzymolysis-free FMDV nonstructural protein mu2C3ABC, wherein the protein has solubility and complete antigenicity, and has a molecular weight of 72 kDa. The protein, as a diagnostic antigen, is prepared into chromatographic strips, has sensitivities of 98.4% and 100% respectively in the detection of FMDV experimentally infected pigs and naturally infection-free pigs, and has specificities of 100% and 98% respectively in the detection of naturally infection-free pigs and vaccine-immunized pigs. Compared with commercial kits Ceditest and UBI, the FMDV 2C3ABC recombinant protein has a high degree of consistency, can be used to distinguish infected animals and immunized animals, wherein K = 0.823 (p is smaller than 0.05).

The invention discloses a DNA methylation detection method of a specific gene segment. The method is characterized in that genome DNA of a biological sample is extracted through a commercial kit method, and the specific gene segment is obtained by using the polymerase chain reaction technology after the genome DNA is subjected to bisulfite conversion; a chemical splitting method or enzymolysis method is used to process the target gene segment product to allow the same to be split from a chain sequence into single basic groups or nucleosides; an internal standard method is used to detect the contents of cytosine and adenine or the nucleosides of the adenine through liquid-phase tandem mass spectrum (LC-MS/MS), and the methylation rate of the target gene segment is calculated according to a formula. By the method, the DNA methylation level of the specific gene segment can be detected fast, efficiently and accurately in a quantified manner.

The invention discloses a method for detecting the ABO blood-group genotype and a template for an allelic ladder of an ABO blood-group gene locus. The method comprises the following steps: taking a genome DNA or general DNA of a to-be-detected sample as a template to perform PCR amplification by adopting primer combination to obtain a PCR amplified product; then, determining which alleles are contained, and further determining the ABO blood-group genotype of the to-be-detected sample, wherein the primer combination consists of 6 primers having nucleotide sequences being sequence 1 to sequence 6 in the sequence table sequentially. Experiments prove that the precision rate for detecting the ABO blood-group genotype by adopting the method is 100 percent; the recombinant plasmid composition provided by the method, serving as a template of an allelic ladder of an ABO blood-group gene locus, can be amplified to obtain the allelic ladder of the ABO blood-group gene locus, and can be applied to various commercial kits. The method and the template have an important application value.

The invention provides a preparation method of amino magnetic nanoparticles and application thereof in DNA (desoxyribonucleic acid) extraction. The preparation method of the amino magnetic nanoparticles comprises the following steps: (1) preparing Fe3O4 magnetic nanoparticles; (2) preparing sodium bitartrate coated Fe3O4 magnetic nanoparticles; and (3) preparing ethidene diamine modified sodium bitartrate coated Fe3O4 magnetic nanoparticles. The amino magnetic nanoparticles prepared by using the preparation method is free of high temperature or high pressure in the synthesis process, are freeof waste, and thus have the characteristics of being green and environment and simple in process; compared with a conventional commercial kit, the nanoparticles are easy in raw material obtaining, lowin price, simple in synthesis step and easy to operate; and the nanoparticles have amino groups on surfaces, are at positive charge states and are beneficial to combination with DNA with negative charges, in the DNA extraction application, the magnetic nanoparticles have good DNA combination capabilities and high extraction efficiency, extraction steps are simple and convenient, and toxic reagents such as chloroform can be avoided.

The invention relates to the field of bioscience, in particular to a diagnostic kit and application of an RNF19 (ring finger protein 19) in preparation of a reagent for early diagnosis of gastric cancers. The diagnostic kit is used for early diagnosis of gastric cancers and comprises an ELISA (enzyme-linked immunosorbent assay) plate, a human protein RNF19, standard serums, an ELISA reagent, an enzyme substrate solution, confining liquid, sample diluent, washing liquid and stop liquid, wherein the human protein RNF19 is enveloped on the ELISA plate. Compared with the prior art, the diagnostic kit has the following advantages: 1. a sensitive, safe, reliable and easy-to-operate commercial kit is provided, is used for qualitatively determining the level of the IgA antibody against RNF19 in the human serums, and is conductive to early diagnosis of gastric cancers in an aided manner; and 2. the provided serum biomarker RNF19 has specificity of 86% and sensitivity of 86% and has the characteristics of high specificity and high sensitivity.

The invention discloses an application of a karyosome protein SP110 and a kit containing the karyosome protein SP110 to the preparation of an early diagnosis reagent for alcoholic cardiomyopathy (ACM). Found by analyzing related blood serum of a patient suffering from the ACM by virtue of the advantages of high throughput and high analysis speed of a human proteome chip and comparing the differences of samples of the patient suffering from the ACM, a patient suffering from dilated cardiomyopathy (DCM) and a healthy person within relatively short time, the expression level of the karyosome protein SP110 in the patient suffering from the ACM is obviously higher than that of each of the patient suffering from the DCM and the healthy person, which hints that the karyosome protein SP110 can beused as a candidate blood serum biomarker of the ACM to realize early diagnosis of the ACM. Experiments prove that the blood serum marker SP110 provided by the invention has the specificity of 80% andthe sensibility of 82% and has the characteristics of high specificity and high sensibility. Further, the invention provides a commercial kit which is sensitive, safe, reliable and easy to operate, and the commercial kit can be used for qualitatively testing the level of IgM antibodies resisting to SP110 in the human blood serum and is beneficial to the early diagnosis of the ACM.

The invention provides a method and a kit for detecting the blood-drug concentration of a PD-1 antibody. With the method provided by the invention, the concentration of the PD-1 antibody is detected by competition of a biotin-labeled PD-1 neutralizing antibody with the PD-1 antibody in a to-be-detected sample through a competitive ELISA method, wherein the biotin-labeled PD-1 neutralizing antibodycompetes with the PD-1 antibody in serum in binding to an antigen immobilized on an elisa plate, and horseradish peroxidase labeled streptavidin is used as a detection antibody to detect the biotin-labeled PD-1 neutralizing antibody, so the PD-1 antibody in the serum is quantified. The method provided by the invention has the advantages of good sensitivity, precision, repeated freeze-thawing stability, room-temperature storage stability, specificity, etc., and facilitates forming a commercial kit. The kit provided by the invention has the advantages of low background, good precision, batch-to-batch consistency, stability, etc.

The invention discloses a connective tissue disease-associated early interstitial lung disease diagnostic kit, comprising a detection module for detecting human peripheral blood MMP7, IFN-gamma and IP-10. The commercial kit provided by the invention is sensitive, safe, reliable and easy to operate; the kit is capable of quantificationally measuring the level of specific protein cytokines and chemotactic factors in human serum, and is helpful for early diagnosis of the connective tissue disease-associated interstitial lung disease.

The invention discloses a colloidal gold immunochromatographic test paper and a preparation and application thereof. The test paper comprises a colloidal gold labelled bovine bluetongue virus vp7 protein, a detection line and a quality control line. The test paper is suitable for detecting a bluetongue virus antibody, a preparation process is optimized through various conditions; the test paper isavailable even when bovine bluetongue standard positive serum is diluted 64 times, and has no cross reaction with bovine tuberculosis, bovine bluetongue, bovine virus diarrhea, bovine pasteurellosisand Enzootic Bovine Leukosis positive serums; and compared with an oversea commercial kit ELISA detection kit (IDEXX), coincidence rate is 98%. The test paper also has relatively better stability, cancomplete a whole detection process within 10 minutes, can quickly detect sample serum and is suitable for field fast check.

The invention relates to a DNA connector. 5' and/or 3' at non-connection tail ends of the DNA connector are sealed. By adopting the DNA connector provided by the invention, non-specific connection ofthe non-connection tail end with a DNA fragment can be avoided, the connection efficiency of the connector with the DNA fragment can be improved, and then the library conversion efficiency can be improved. A preparation method of the DNA connector provided by the invention is simple, a library construction method is simple, efficient and low in cost, and the high expense of transformation enzymesor commercial kits can be avoided.