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Colloidal gold immunochromatographic test paper and preparation and application thereof

A technology of immunochromatography test strips and colloidal gold, which is applied in the biological field, can solve the problems of few reports on colloidal gold immunochromatography test strips, and achieve good stability

Inactive Publication Date: 2019-01-22
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the diagnosis of animal diseases, there are few reports on colloidal gold immunochromatographic test strips for the detection of bluetongue virus antibodies

Method used

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  • Colloidal gold immunochromatographic test paper and preparation and application thereof
  • Colloidal gold immunochromatographic test paper and preparation and application thereof
  • Colloidal gold immunochromatographic test paper and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. Preparation of colloidal gold immunochromatographic test paper for bluetongue virus antibody detection

[0038] There are many factors affecting the quality of colloidal gold immunochromatographic test paper, such as the size of colloidal gold particles, the amount of colloidal gold-labeled antigen, the concentration of coated antibody, the type of NC membrane and glass fiber membrane, the preparation of buffer, etc. Due to factors such as steric hindrance, different labels require different particle sizes of colloidal gold. The size of colloidal gold particles, ionic strength, and molecular weight of proteins all affect the binding of colloidal gold to proteins.

[0039]In the rapid detection of colloidal gold immunochromatography, NC membrane is used as a solid support for coating antibodies, and its type selection is very important. It needs to be comprehensively screened from the aspects of chromatography speed, sensitivity and non-specific binding. An N...

Embodiment 2

[0060] Embodiment 2, the performance detection of colloidal gold immunochromatography test paper for bluetongue virus antibody detection

[0061] 1. Test strip specificity experiment

[0062] Detect bovine tuberculosis positive serum, bovine brucellosis positive serum, bovine viral diarrhea positive serum, bovine pasteurellosis positive serum with 50 times dilution of PBS buffer solution simultaneously with the test strip prepared in embodiment 1 step 5 With the positive serum of bovine leukemia, the standard negative serum of bovine bluetongue disease is used as negative control, and the standard positive serum of bovine bluetongue disease is used as positive control to observe the intersectionality of several diseases and judge the specificity of the test strip.

[0063] The result is as figure 2 Shown, show that bluetongue and tuberculosis, bovine brucellosis, bovine viral diarrhea, bovine pasteurellosis and bovine leukemia all have no cross-reactivity, illustrating that ...

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Abstract

The invention discloses a colloidal gold immunochromatographic test paper and a preparation and application thereof. The test paper comprises a colloidal gold labelled bovine bluetongue virus vp7 protein, a detection line and a quality control line. The test paper is suitable for detecting a bluetongue virus antibody, a preparation process is optimized through various conditions; the test paper isavailable even when bovine bluetongue standard positive serum is diluted 64 times, and has no cross reaction with bovine tuberculosis, bovine bluetongue, bovine virus diarrhea, bovine pasteurellosisand Enzootic Bovine Leukosis positive serums; and compared with an oversea commercial kit ELISA detection kit (IDEXX), coincidence rate is 98%. The test paper also has relatively better stability, cancomplete a whole detection process within 10 minutes, can quickly detect sample serum and is suitable for field fast check.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a colloidal gold immunochromatographic test paper for detecting bluetongue virus antibodies and a preparation method and application thereof. Background technique [0002] Bluetongue (BT) is an important insect-borne infectious disease caused by bluetongue virus (BTV), which mainly affects sheep or cattle and some deer populations. Hyperemia, swelling, and formation of petechiae, which later turn blue-purple, are associated with high morbidity and mortality. BTV was first discovered in 1876. At present, BTV has been isolated in many tropical and temperate countries, and the distribution of the disease is constantly expanding. It has become an international widespread animal disease, showing a global distribution. trend. According to the statistics of the World Organization for Animal Health (OIE), there are many countries in the world where animals have been infected and confirmed ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/569
CPCG01N33/56983G01N33/577G01N33/6893
Inventor 韩雪清王慧煜孔玉方梅琳林祥梅吴绍强
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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