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Diagnostic kit and application of RNF19 (ring finger protein 19) in preparation of reagent for early diagnosis of gastric cancers

A diagnostic kit and technology for gastric cancer, applied in the field of biological sciences, can solve problems such as the inability to achieve high-sensitivity and high-specificity diagnosis of gastric cancer, and achieve the effect of easy operation and high specificity

Active Publication Date: 2012-09-19
广州博翀生物科技有限公司
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Problems solved by technology

[0008] The first object of the present invention is to provide a diagnostic kit to solve the technical problem that the gastric cancer diagnostic kits in the prior art cannot realize early diagnosis of gastric cancer with high sensitivity and high specificity
[0009] The second object of the present invention is to provide an application of RNF19 in the preparation of early gastric cancer diagnostic reagents, so as to solve the technical problem that the gastric cancer diagnostic reagents in the prior art cannot achieve early diagnosis of gastric cancer with high sensitivity and high specificity

Method used

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  • Diagnostic kit and application of RNF19 (ring finger protein 19) in preparation of reagent for early diagnosis of gastric cancers
  • Diagnostic kit and application of RNF19 (ring finger protein 19) in preparation of reagent for early diagnosis of gastric cancers
  • Diagnostic kit and application of RNF19 (ring finger protein 19) in preparation of reagent for early diagnosis of gastric cancers

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Embodiment Construction

[0038] The present invention will be further described below in conjunction with the embodiments and the accompanying drawings.

[0039] 1. Expression, purification and identification of RNF19:

[0040] The protein RNF19 is obtained by overexpression induced by genetically engineered Saccharomyces cerevisiae using galactose, and then separated and purified by agarose affinity medium (glutathione), and the results of silver staining and Western-Blotting identification Respectively as figure 1 and figure 2 shown.

[0041] 2. Preparation of serum samples:

[0042] Centrifuge the whole blood sample at 1000g for about 20 minutes after standing at room temperature for 2 hours or overnight at 4°C, and take the supernatant for immediate detection; or aliquot and store the sample at -20°C or -80°C, but avoid Freeze and thaw repeatedly. Thawed samples should be centrifuged again prior to testing. The tested samples cannot contain NaN 3 , because NaN 3 Inhibits horseradish pero...

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Abstract

The invention relates to the field of bioscience, in particular to a diagnostic kit and application of an RNF19 (ring finger protein 19) in preparation of a reagent for early diagnosis of gastric cancers. The diagnostic kit is used for early diagnosis of gastric cancers and comprises an ELISA (enzyme-linked immunosorbent assay) plate, a human protein RNF19, standard serums, an ELISA reagent, an enzyme substrate solution, confining liquid, sample diluent, washing liquid and stop liquid, wherein the human protein RNF19 is enveloped on the ELISA plate. Compared with the prior art, the diagnostic kit has the following advantages: 1. a sensitive, safe, reliable and easy-to-operate commercial kit is provided, is used for qualitatively determining the level of the IgA antibody against RNF19 in the human serums, and is conductive to early diagnosis of gastric cancers in an aided manner; and 2. the provided serum biomarker RNF19 has specificity of 86% and sensitivity of 86% and has the characteristics of high specificity and high sensitivity.

Description

technical field [0001] The invention relates to the field of biological sciences, in particular to a diagnostic kit and the application of RNF19 in the preparation of early-stage diagnostic reagents for gastric cancer. Background technique [0002] Gastric cancer is a common malignant tumor that endangers human health. According to the statistics of the World Health Organization (WHO) in 2008 (World Health Organization Annual Statistics, 2008), the death rate of gastric cancer patients ranks second in the world. In 2006 WHO statistics showed nearly 950,000 new cases and about 700,000 deaths from this disease. In my country, there are more than 300,000 new gastric cancer patients each year, and about 160,000 people die from gastric cancer. The mortality rate ranks first among malignant tumors, which seriously threatens people's lives and health (Chen Zhu, the third national retrospective sampling survey report on causes of death, 2008) . In order to reduce the mortality rate...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 杨丽娜陶生策刘炳亚王靖方郭书娟朱正纲李建芳燕敏
Owner 广州博翀生物科技有限公司
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