Diarrhetic shellfish toxin high throughput detection device and method based on image analysis

Abstract

The invention discloses a diarrhetic shellfish toxic high throughput detection device and method based on image analysis. The device comprises a power supply adapter, a wide-angle lens, an intelligent mobile facility, a first darkroom, a second darkroom, a commercial reagent detection plate, a detection plate loading stage, and an electroluminescent panel. The method comprises the following steps: preparing a sample solution of diarrhetic shellfish toxin, then preparing a standard solution, using a commercial kit to prepare a detection plate to be detected; collecting and analyzing detection images to calculate the color ratio (CR) of R component of RGB space in a detection plate hole image; drawing a calibration curve of the commercial kit to detect the diarrhetic shellfish toxin through a least square method; obtaining the hole image CR value of the sample solution, and substituting the CR value of the sample solution into the calibration curve to calculate the concentration of the diarrhetic shellfish toxic of the sample solution. The provided device achieves the high throughput quantitative detection of diarrhetic shellfish toxic, and has the advantages of simple operation steps, low cost, and suitability for on-site detection.

Description

technical field [0001] The invention relates to a high-throughput detection device and technology for simultaneously detecting shellfish diarrheal toxins, in particular to a high-throughput detection device and method for shellfish diarrheal toxins based on image analysis. Background technique [0002] Shellfish diarrheal toxin is a kind of highly toxic non-protein toxin, which is a kind of highly toxic non-protein toxin, which can cause poisoning and even death after eating. At present, domestic shellfish diarrhea toxin detection methods are usually used in mouse biological detection method, liquid chromatography-mass spectrometry method and traditional Elisa kit detection method. The large individual variability of the mouse bioassay method makes it difficult to estimate the toxicity, and the liquid chromatography-mass spectrometry method is cumbersome to operate and has huge equipment, which cannot meet the requirements of on-site detection. The detection instrument used...

AI Technical Summary

Problems solved by technology

The large individual variability of the mouse bioassay method makes it difficult to estimate the toxicity, and the liquid chromatography-mass spectrometry method is cumbersome to operate and has huge equipment, which cannot meet the requirements of on-site testing

The detection instrument used in the traditional Elisa kit detection method is a microplate reader, which is still too large to adapt to on-site detection, and there are differences in the enzyme-linked reaction with different incubation times. The time-sharing detection method adopted by the microplate reader The method will cause the difference in the detection results between the wells

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