Chemiluminescence detection kit and chemiluminescence detection method thereof

Abstract

The present invention belongs to the field of biochemical experimental reagents, and particularly relates to a chemiluminescence detection kit and a chemiluminescence detection method thereof. The chemiluminescence detection kit is composed of an A solution and a B solution, wherein the A solution is a buffer containing 2.5 mM luminol and 0.396 mM p-coumaric acid, and the B solution is a buffer containing 0.02% hydrogen peroxide. According to the luminescence detection kit provided by the present invention, the protein of each concentration can be better detected, and the detection sensitivityand intensity are nearly 20 times that of the common commercial kit on the market, so that the effects of reducing the dosage of the antibody and saving the experiment cost are achieved.

Description

technical field [0001] The invention belongs to the field of biochemical experiment reagents, in particular to a chemiluminescence detection kit and a chemiluminescence detection method thereof. Background technique [0002] Western blotting (Western Blot) was proposed by Harry Towbin of the Friedrich Miescher Institute in Switzerland in 1979. A commonly used experimental method in science. The basic principle is to color the cells or biological tissue samples treated by gel electrophoresis through specific antibodies, and it is widely used in gene expression research at the protein level, antibody activity detection, and early diagnosis of diseases. [0003] Enhanced substrate chemiluminescence (ECL, Enhanced chemiluminescence) is a Western Blot chromogenic method that has developed very rapidly in the field of biotechnology in recent years, and is an ultra-high-sensitivity micro-measurement technology. It has the advantages of high sensitivity, wide detection range, simp...

AI Technical Summary

Problems solved by technology

However, the existing chemiluminescence detection kits on the market are not only expensive but also poor in sensitivity, and are not suitable for general use in laboratories.

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