46 results about "Spermatogenic Cell" patented technology

The invention discloses a method for predicting whether sperms exist in the testis of an azoospermia patient or not according to seminal plasma mRNA. The method comprises the following steps of dividing seminal fluid specimens of the azoospermia patient into a testicular sperm group and a testicular azoospermia group by taking a testicular biopsy as a gold standard, determining the difference of exRNA expression profiles of two seminal fluid groups, carrying out comparison with an existing spermatogenic cell transcriptome database, and searching for differentially expressed exRNA capable of judging spermatogenic cell types, which is used for judging whether haploid sperm cells exist in the testis of the azoospermia patient or not. The clinical application of the scheme provided by the invention can greatly improve the sperm taking success rate of the azoospermia patient, and is also helpful for guiding the azoospermia patient to select an economical, painless and effective treatment method to solve the problem of infertility of the azoospermia patient.

The invention relates to a radioprotective medicament, and particularly relates to a radioprotective medicament prepared by using traditional Chinese medicine as raw material herbs. The medicament is used to treat and prevent problems of reproductive system radiation hazard, and comprises the following herbs by mass: 90-150 parts of medlar, 90-150 parts of semen cuscutae, 40-70 parts of cistanche, 40-70 parts of raspberry, 25-40 parts of prepared rehmannia roots. The medicament of the invention can be prepared into any commonly used oral dosage form by routine traditional Chinese medicine preparation methods, such as pills, powder, tablets, capsules, oral liquid, etc. The medicament has the efficacy of tonifying kidney, replenishing essence, and tonifying qi, and has obvious effect on ionizing radiation resistance and spermatogenic cell protection.

The invention discloses a method for screening and identifying tumor/testis antigens based on monoclonal antibodies with spermatogenic cell specificities. Spermatogenic cells of human bodies are used as immune antigens, monoclonal antibodies are prepared by the aid of the lymphocyte hybridoma technology, the monoclonal antibodies with spermatogenic cell specificities are screened by the aid of immunohistochemistry, and then the expression distribution law of antigens identified on the screened monoclonal antibodies on tumor tissues and other normal tissues is researched on a protein level. The monoclonal antibodies are selected for the expression law of the tumor/testis antigens by the aid of immunohistochemical staining on the basis to be used as candidate antibodies for identifying the tumor/testis antigens, and new tumor/testis antigen molecules recognized by the monoclonal antibodies can be identified by the aid of proteomics technologies such as immunoprecipitation-mass spectrum analysis and the like.

The invention relates to an application of seabuckthorn fruit oil and/or whole fruit oil in preparing medicine promoting the formation of male sperms, preventing the apoptosis of spermatogenic cells or promoting the formation of female ovarian follicles and the maturation of germ cells and treating male and female sterility. By taking the preparation, kidney deficiency and infirmity and various chronic diseases can be recovered to the health status, more effectively, the invention has the functions of promoting the formation of the male sperms and protecting the quantity of the spermatogenic cells, and preventing the apoptosis of spermatogenic cells, and can obviously increase the quantity and the motor ability of the sperms, and increase the weight of spermary, and simultaneously has the functions of promoting the formation of the female ovarian follicles and the maturation of the germ cells, thereby being used as the medicine for the clinical treatment of male and female sterility. Compared with other medicines, the medicine has simple ingredients, belongs to target organ excitant, and has the reversible automatic regulation function of a second messenger, thereby being the medicine with high curative effect, security, and simplification.

The invention discloses a rapid typing detection kit for ejaculated sperm cells. The kit comprises a PBS solution, a calcium-free HBSS buffer solution, a fixing solution, a staining reagent and a quality control product. The PBS solution and the calcium-free HBSS buffer solution both comprise one or more of bovine serum albumin, fetal bovine serum, glycerol serum, protein and glycerol; the staining reagent comprises a cell permeabilizing agent and a fluorescently labeled antibody. The invention also provides a rapid typing detection method for ejaculated sperm cells by adopting the kit mentioned above. The rapid typing detection kit for ejaculated sperm cells and the rapid typing detection method for ejaculated sperm cells can be used for quickly determining the types and the quantity of spermatogenic cells in the ejaculated sperm cells, further determining whether the ejaculated sperm cells are obstructive or non-obstructive azoospermia, solve the problem that clinical evaluation aiming at the current semen cytology in China lacks a quick and accurate detection reagent in the current clinic, provide guidance for a clinician to select a treatment scheme, and practically solve the problem of a patient.

The invention provides a spermatogenic pill having the functions of warming yang, tonifying kidneys and strengthening the essence. The spermatogenic pill is mainly used for treating azoospermatism. The spermatogenic pill has excellent treatment effect for patients having spermatogenic cells without vas deferens obstruction. The traditional Chinese medicine composition is prepared from the following more than twenty medicinal raw materials by weight: herba epimedii, semen cuscutae, the fruit of Chinese wolfberry, desertliving cistanche, fish glue, goat testicle, male silk moth, human placenta, semen allii tuberose, red ginseng, fleece-flower root, rehmannia glutinosa, rhizoma curculiginis, rhizoma curculiginis, fructus psoraleae, cinnamon, cooked monkshood, angelica sinensis, the root of red-rooted salvia and the like. The spermatogenic pill targets man sterility, has the functions of warming yang, tonifying kidneys and strengthening the essence and is mainly used for treating azoospermatism. The spermatogenic pill can take effect quickly for patients having spermatogenic cells without vas deferens obstruction. Natural Chinese herbal medicines does not have side effects on the body. The formula has the functions of strengthening the essence and effectively increasing the number of sperms.

The invention provides application of geniposide in preparation of a medicine for treating asthenospermia. Experimental results show that the geniposide can significantly improve the sperm quantity and activity and reduce the sperm malformation rate, can significantly inhibit endoplasmic reticulum stress inside testis, increase mitochondrial membrane potential, regulate expression of related proteins in testis tissues, and improve excessive apoptosis of spermatogenic cells.

The invention provides a dissociation and separation method for shellfish spermary spermatogenic cells, and belongs to the technical field of the separation of ocean shellfish cells. The dissociationand separation method comprises the following steps of: the spermary of a shellfish during a proliferating phase is broken and is digested by a collagenase IV solution of which the mass concentrationis 0.1% and a trypsin solution of which the mass concentration is 0.25% in sequence, and spermary dissociation is carried out to obtain a single-cell suspension; and Percoll solutions of which the volume concentration is independently 10%, 22% and 35% is prepared, and the Percoll solutions are loaded in the same centrifuge tube in a descending order, the single-cell suspension of the shellfish spermatogenic cells is added into the top layer of the centrifuge tube, and centrifugation is carried out to independently collect three layers of cells. By use of a two-step enzymolysis method, spermatogenic cells in spermary tissues can be successfully dissociated into the single-cell suspension, a cell survival rate can be as high as 96.62%, and the cells can be normally subjected to in vitro normal culture. Meanwhile, high-purity spermatogonia and spermatocytes can be separated, an important material is provided for researching a shellfish gamete generating and breeding mechanism, and the dissociation and separation method has a high guidance meaning and a good application value.

The invention discloses application of a P substance in preparation of a drug for treating male infertility. Treatment of an azoospermia mouse model by the P substance finds that the P substance can effectively improve spermatogenic functions of the azoospermia mouse model caused by busulfan and further proves that the P substance improves the spermatogenic functions by promoting proliferation ofspermatogenic cells. The invention provides a new thought and theoretical support for treatment of male infertility.

A method for detecting the nucleoproteins of spermatogenic cells through utilizing indirect immunofluorescent staining comprises the following steps: separating convoluted seminiferous tubules from the testis tissues of an animal, utilizing a hypotonic solution to process spermatogenic cells, and detecting the nucleoproteins of the spermatogenic cells through adopting indirect immunofluorescent staining, wherein the hypotonic solution comprises 17mM of sodium citrate, 50mM of sucrose, 5mM of EDTA, 0.5mM of DTT, 0.5mM of PMSF and 30mM of Tris and having a pH value of 8.2. The method uses the hypotonic solution to carry out permeable treatment of the cells in order to rapidly expand the cells, disperse chromatins and fully release the proteins in cell nuclei, so the spermatogenic cells can well combine with primary antibodies, thereby the combination efficiencies of secondary antibodies are enhanced, fluorescent staining is complete and full, and the preparation of spermatogenic cell chromosomes and karyotyping are successfully carried out.

The invention provides a method for promoting the development of sexual organs and sperms of sires and feeds vitamin E to the sires from the weaning to the sexual maturity of the sires. The method of the invention can increase the weight of epididymides, the density of convoluted seminiferous tubules, the diameter of epididymides and the amount of spermatogenic cells, use the sires beforehand and improve the reproductive capacity and utilization years of herd sires. Additionally, the invention can also reduce tedious operations in keeping and improving semen quality and sperm motility in artificial insemination, improve the fresh precision quality used in artificial insemination and frozen semen so as to improve the quality of the artificial insemination and frozen semen, thus increasing the production efficiency from the source. The invention is easy and convenient in operation and significant in economic benefits.

The invention discloses application of composite amino acid in preparing a medicine for treating oligoasthenozoospermia. The composite amino acid comprises the following main medicine components: leucine, isoleucine, lysine hydrochloride, phenylalanine, threonine and the like. Test results show that (1) the composite amino acid has a remarkable protection function on dyszoospermia caused by GTW (Multiglycosides of Tripterygium wilfordii Hook.f.), oligoasthenozoospermia caused by GTW can be alleviated, morphological changes of testis and epididymis can be reduced, and spermatogenic cell apoptosis can be inhibited; (2) testis functions of rats suffering from oligoasthenozoospermia caused by GTW can be remarkably improved, a normal reproduction hormone level can be maintained, and normal functions of hypothalamus-hypophysis-testis gonad axes can be protected; (3) an energy metabolism level of a reproductive system of a rat suffering from oligoasthenozoospermia caused by GTW can be remarkably increased, energy metabolism of sperms can be improved, and sperm quality can be improved; (4) antioxidation levels of testis and epididymis can be increased, and sperm quality can be improved; the composite amino acid can be used as an active component to prepare medicines for treating oligoasthenozoospermia.

The invention discloses an in-vitro separation method for primary spermatogenic cells of rats. The method comprises the following steps: 1) collecting the testis on two sides of a fresh in-vitro rat, and stripping envelopes and blood vessels to obtain testis tissues; 2) digesting the testicular tissue to prepare a single-cell suspension; 3) culturing the single-cell suspension until non-spermatogenic cells are adhered to the wall, sucking out non-adhered suspension cells, and separating the suspension cells by adopting a Percoll method to obtain the spermatogenic cells. According to the method, a foundation is laid for in-vitro research on the reproductive function of the rat testis, in-vitro separation and culture of the rat testis spermatogenic cells are achieved, and it is proved that the spermatogenic cells separated and cultured through the method are high in in-vitro survival rate and long in survival time. Normal rat testicular spermatogenic cells can be obtained by using the method, the cost is low, the operation is simple, the repeatability is high, various experimental requirements can be met, and the method is worthy of large-scale popularization.

The invention is suitable for the technical field of biological cryopreservation, and provides a bovine testis tissue serum-free cryopreservation protective agent and a cryopreservation method and application thereof. The serum-free cryopreservation protective agent comprises the following components: a Knockout DMEM medium, a KSR serum substitute, dimethyl sulfoxide and mycillin. According to theinvention, the Knockout DMEM culture medium which does not contain serum is used as a substrate, the kSR serum substitute, dimethyl sulfoxide and mycillin are used as low-temperature protective agents in a combined manner, the cost of the cryopreservation protective agent can be reduced, the risk of exogenous pathogen and virus pollution is avoided, in addition, the bovine testis tissue can be preserved at low temperature for a long time, cell apoptosis is reduced, the tissue form is maintained, spermatogenic cells and testis cells are well protected, the testis tissue after cryopreservationand resuscitation is complete in structure, the activity of various testis cells is high, and normal proliferation and development can be achieved.

The invention provides an antisense RNA sequence combination and method for inhibiting gonad development of pelteobagrus fulvidraco and application, and belongs to the technical field of molecular biology and breeding biology. The antisense RNA sequence combination for inhibiting gonad development of the pelteobagrus fulvidraco provided by the invention comprises antisense RNA for inhibiting an FSH receptor. The antisense RNA sequence combination can inhibit mRNA transcription of the FSH receptor of the pelteobagrus fulvidraco, thereby interfering with the generation and development of oocytesand spermatogenic cells, inhibiting gonad development of the pelteobagrus fulvidraco, and greatly shortening the marketing period of the pelteobagrus fulvidraco; and the antisense RNA sequence combination provided by the invention can also realize effective and accurate regulation and control of expression of a target gene, has extremely small damage to ova, is high in success rate and stable inbred offspring phenotype, and has an extremely good application prospect.

The invention discloses an in-vitro culture method for cryopreserved bovine spermatogenic cells, which comprises the following steps: performing programmed freezing or vitrification freezing on fresh bovine testicular tissues; resuscitating bovine testis tissues, and then preparing a single-cell suspension; preparing feeder layer cells by using the prepared single-cell suspension; and co-culturing supporting cells and spermatogenic cells by using the prepared feeder layer cells. Compared with the prior art, the method has the advantages that sufficient samples can be effectively collected at a time and can be stored and transported under the low-temperature condition, and the spermatogenic cells with high motility rate can be obtained by performing enzyme digestion on the resuscitated samples. The spermatogenic cells subjected to resuscitation can be subjected to long-term culture and passage in vitro, and a foundation is laid for subsequent cell experiments. The method has the characteristics of simplicity in operation and no limitation of time and distance, and can reduce sampling times and cost.