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In-vitro separation method for primary spermatogenic cells of rats

A technology of germ cells and cells, which is applied in the field of in vitro separation of primary rat germ cells, can solve the problems of small number of germ cells, difficulty in meeting experimental requirements, time-consuming and labor-intensive problems, and achieve high repeatability, low cost, Simple operation effect

Pending Publication Date: 2021-11-16
XI AN JIAOTONG UNIV
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Problems solved by technology

The number of spermatogenic cells obtained in a single experiment using this extraction method is small, it is difficult to meet the experimental needs, and it is time-consuming and laborious

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  • In-vitro separation method for primary spermatogenic cells of rats

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Embodiment Construction

[0023] In order to enable those skilled in the art to better understand the solutions of the present invention, the following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only It is an embodiment of a part of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0024] It should be noted that the terms "first" and "second" in the description and claims of the present invention and the above drawings are used to distinguish similar objects, but not necessarily used to describe a specific sequence or sequence. It is to be understood that the data so used are interchangeable under appropriate circums...

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Abstract

The invention discloses an in-vitro separation method for primary spermatogenic cells of rats. The method comprises the following steps: 1) collecting the testis on two sides of a fresh in-vitro rat, and stripping envelopes and blood vessels to obtain testis tissues; 2) digesting the testicular tissue to prepare a single-cell suspension; 3) culturing the single-cell suspension until non-spermatogenic cells are adhered to the wall, sucking out non-adhered suspension cells, and separating the suspension cells by adopting a Percoll method to obtain the spermatogenic cells. According to the method, a foundation is laid for in-vitro research on the reproductive function of the rat testis, in-vitro separation and culture of the rat testis spermatogenic cells are achieved, and it is proved that the spermatogenic cells separated and cultured through the method are high in in-vitro survival rate and long in survival time. Normal rat testicular spermatogenic cells can be obtained by using the method, the cost is low, the operation is simple, the repeatability is high, various experimental requirements can be met, and the method is worthy of large-scale popularization.

Description

technical field [0001] The invention belongs to the technical field of cell culture and separation, and relates to a method for separating primary rat spermatogenic cells in vitro. Background technique [0002] Nearly 10%-15% of couples of childbearing age in the world are troubled by infertility, of which about 50% are caused by male factors. Multiple studies have shown that despite regional and ethnic differences, human sperm concentration has declined substantially over the past few decades, and sperm quality and fertility have significantly decreased. A number of studies have shown that related genetic material disorders may be the main cause of spermatogenesis disorders. Therefore, the study of genetic and molecular mechanisms related to abnormal spermatogenesis will lay the foundation for elucidating the etiology of idiopathic male infertility and promoting the development of related diagnosis and treatment techniques. [0003] Spermatogenesis is a complex and unique...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/076
CPCC12N5/061C12N2509/00C12N2509/10
Inventor 周党侠葛攀张健杨妍琪吕茉琦
Owner XI AN JIAOTONG UNIV
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