229 results about "Male fertility" patented technology

Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants. In one such method, the homozygous recessive condition of male sterility causing alleles is maintained after crossing with a second plant, where the second plant contains a restoring transgene construct having a nucleotide sequence which reverses the homozygous condition. The restoring sequence is linked with a hemizygous sequence encoding a product inhibiting formation or function of male gametes. The maintainer plant produces only viable male gametes which do not contain the restoring transgene construct. Increase of the maintainer plant is also provided by self-fertilization, and selection for seed or plants which contain the construct.

A kit for testing male fertility comprises a vessel, a base unit, a liquid supply containing liquid and two filters. The first filter is a sample separation filter which forms a hindrance to transmission of spermatozoa. The second filter of the kit is a spermatozoa detection filter comprising a reagent for identifying spermatozoa. Activation of the kit is prevented until a transport medium, such as the liquid, fills a gap allowing spermatozoa to transmit to a detection zone. The kit may be of one-piece construction and utilizes a thin piece of filter material to separate motile from non-motile spermatozoa.

Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.

Test kits for assessing male fertility include a sample holder defining an object plane, a lens, and a two dimensional light sensor defining an image plane arranged along a common linear axis. The distance between the object plane and the image plane may be no more than 50 mm, and may be no more than 30 mm. A lens aperture may have an area of 1-10 mm2. The test kit may have a housing with a maximum linear dimension of no more than 100 mm. Processing circuitry may be provided that is configured to produce a sperm count and / or sperm motility measurements by processing image data from the two-dimensional light sensor.

The invention discloses a thermo-sensitive male fertility gene and an application thereof and also discloses a genetic marker of the thermo-sensitive male fertility gene, and provides a protein coded by the thermo-sensitive male fertility gene and a carrier containing the thermo-sensitive male fertility gene and a recombinant microorganism. The invention also discloses the application of the thermo-sensitive male fertility gene in cultivating a thermo-sensitive male sterile line and an application of the genetic marker in rice breeding. The thermo-sensitive fertility gene TSSNR is acquired through the map-based cloning technique of Annong S-1 thermo-sensitive sterility gene locus tms5, functions of the gene are validated through transgene experiments, and the transgene technique of RNAi or antisense RNA or the over-expression dominant negative principle is provided, functions of the TSSRZ gene in normal rice varieties are incapable, so as to cultivate the thermo-sensitive male sterile line, and the application value is significant.

The present invention relates to isolated male tissue-preferred regulatory sequences from Ms45 gene. The invention also relates to the use of Ms45 gene male tissue-preferred regulatory sequences in mediating male fertility in a plant, and in producing hybrid seed. The Ms45 male tissue-preferred regulatory region can be operably linked to exogenous genes encoding cytotoxins or inhibitory molecules. The invention also relates to plant cells, plant tissue and plants that contain the MS45 gene regulatory sequences.

Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants.

Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants.

Nucleotide sequences of a Msca1 gene, critical to male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in impacting male fertility in plants.

The present invention relates to methods for enhancement of naturally occurring cytoplastic male sterility and for restoration of male fertility and uses thereof in hybrid crop production. There is also disclosed a method for restoration of male fertility to cytoplasmic male sterile plants; which comprises the steps of: a) introducing into the nucleus of a plant cell a gene construct essentially consisting of a sequence encoding a mitochondrial transit peptide fused upstream of and in frame with an edited form of a normal mitochondrial gene that is co-transcribed with an usual CMS-associated mitochondrial gene; b) selecting for plant cells that have acquired the gene construct in step a); and c) inducing regeneration of selected plant cells to produce a mature plant.

The invention relates to the field of plant breeding, and particularly relates to the fertility restoration of a homozygous recessive genic male sterility rice plant. Furthermore, the invention relates to a method for establishing a plant male sterile line and a maintainer line and a genetic transformation material, and more particularly relates to a structure, a rice cell, tissue or organ, a method for establishing a rice male sterile line and a maintainer line, a method for restoring the male fertility of a rice sterile plant, a method for preparing rice seeds, a genetic transformation material, a method for preparing hybrid rice and an application of the rice male sterile line in preparation of hybrid rice.

The invention relates to a preparation method of an MACA extract. The preparation method comprises following steps: (1) extracting MACA with a solvent to obtain a primary MACA extract as a purification raw material; and (2) continuously performing purification to obtain the MACA extract being high in the content of MACAmide, wherein the purification is carried out in a column chromatography manner. The column chromatographic purification comprises following steps: (1) performing impurity washing to remove impurities; and (2) performing elution to obtain the MACAmide. The MACA extract is high in the content of the MACAmide so that the MACA extract is excellent in effects of enhancing male fertility and treating male sexual dysfunction. The preparation method is simple in extraction technology, is easy to operate, has a strong adaptability in production equipment and is easy to achieve industrialized production.

The invention discloses a gene MS33 related to maize male nuclear sterility and application thereof in cross breeding. The invention provides MS33 protein related to maize male nuclear sterility, which is shown in (a) or (b): (a) protein composed of an amino acid sequence shown in a sequence 1 in a sequence table; or (b) protein which is derived from the sequence 1 by substituting and / or deleting and / or adding one or more amino acid residues on the amino acid sequence of the sequence 1, and related to plant male fertility. MS33 gene mutation causes maize complete male sterility, characters are complete, and environmental influence is avoided. The gene has application potential in maize hybrid seeds.

The invention discloses a plant male fertility related protein and a coded gene and application thereof. The protein is of (a) or (b): (a) the protein formed by an amino acid sequence indicated in SEQ ID NO.1; and (b) the protein related to plant male fertility and derivate from the SEQ ID NO.1 by using one or a plurality of amino acid residues to replace and / or delete and / or add in the amino acid sequence 1. The plant male fertility related protein influences the anther cracking process of plants. Plant male fertility can be reduced and maturing rate can be affected by restraining expression of the coded gene of the protein, and accordingly transgenic plants with male anther non-cracking variation and plant male sterile line transgenic plants can be cultured. Male fertile transgenic plants can be cultured by introducing the coded gene of the protein into male sterile plants. The protein and the coded gene of the protein can be applied to plant genetic improvement.

The invention provides a rice temperature-sensitive nuclear sterility gene tms3 mutant and its molecular marker and an application thereof. The rice temperature-sensitive nuclear sterility gene tms3 is subjected to map-based cloning, and the function of the gene is verified by genetic transformation. The nucleotide sequence of the temperature-sensitive male fertility gene tms3 mutant provided by the present invention is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. A Crispr technology is used to induce the loss of tms3 gene function in normal rice varieties to obtain mutant plants, and a thermosensitive male sterility line can be cultivated, which proves that the gene has important application value. The invention also provides the molecular marker closely linked to the tms3 gene, and the molecular marker can be used to detect tms3, and the application in the cultivation of the temperature-sensitive nuclear sterility and the rice two-line sterile line can be realized.

The invention provides a genetic intelligent seed breeding and production system for crossbreeding and production of crops and application thereof. The system contains a GAT system vector, and the vector comprises five functional element expression cassettes: a plant male fertility restoration gene element expression cassette used for restoring male fertility of recessive genic male sterility mutants, a plant pollen abortion gene element expression cassette used for removing pollen containing GAT and keeping a heterozygotic state or a hemizygotic state of a GAT maintainer line, a chemical herbicide positive selection expression cassette used for gene transformation and GAT maintainer line impurity removal and purification, a chemical herbicide negative selection expression cassette used for removing herbicide-sensitive GAT maintainer line pollen and seed escape and removing impurities and purifying a GAT sterile line, and a seed screening element expression cassette used for mechanically sorting seeds. The system can be used for crossbreeding and hybrid seed production of plant recessive genic male sterility materials, so that new varieties of plants with high quality, high yield, wide applicability and high resistance and seeds of the plants are obtained.

A method and device for assaying sperm motility in a forward direction and density of active sperm in a semen sample are disclosed. The device includes a microfluidics structure having a sample reservoir, a downstream collection region and a microchannel extending therebetween. The microchannel is dimensioned to confine sample sperm to single-direction movement within the channel, such that sperm in a semen sample placed in the sample reservoir enter and migrate along the microchannel toward and into the collection region. Also included is a detector for detecting the presence of labeled sperm in the microchannel or collection region, and an electronics unit operatively connected to the detector for (i) receiving detector signals, (ii) based on the detector signals received, determining sperm motility and density in the sperm sample, and (iii) displaying information related to sperm motility and density.

The invention relates to a rice temperature sensitive male sterility gene, a coding protein and application thereof. The rice temperature sensitive male sterility gene has one of the following nucleotide sequences, i.e. 1, a nucleotide sequence shown in SEQ IN NO1, and 2, a NDA sequence which is shown in SEQ IN NO3, has highest homology with a base sequence of an arabidopsis gene and an expected value of 1.8e-27 and is used for coding same biological functional proteins. The transcription expression of the gene OsATMS1 has a characteristic of being sensitive to the environment temperature, can ensure that the plant male fertility has a temperature sensitive characteristic, is expected to have an application value on two aspects of crop crossbreeding and yield increasing.

Nucleotide sequences of a Msca1 gene, critical to male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in impacting male fertility in plants.

Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants. In one such method, the homozygous recessive condition of male sterility causing alleles is maintained after crossing with a second plant, where the second plant contains a restoring transgene construct having a nucleotide sequence which reverses the homozygous condition. The restoring sequence is linked with a hemizygous sequence encoding a product inhibiting formation or function of male gametes. The maintainer plant produces only viable male gametes which do not contain the restoring transgene construct. Increase of the maintainer plant is also provided by self-fertilization, and selection for seed or plants which contain the construct.

This disclosure provides a method for determining male fertility status. The method comprises determining GM1 localization patterns following induced sperm capacitation, identifying the percentage of various patterns, particularly the ratio of [(AA+APM) / total number of GM1 localization patterns] and determining if the percentage of certain GM1 localization patterns in response to induced capacitation is altered. Based on the change in the percentage of localization patterns of certain patterns in response to induced capacitation, alone or in combination with other sperm attributes, male fertility status can be identified.

A 22 kD sperm protein, SP22, correlates with fertility and predicts fertility in males. The protein can be assayed to detect decreases in fertility resulting from exposure to toxicants and pollutants which are known or suspected to decrease fertility. In an antibody is generated to this protein, the antibody recognition by sperm in an epididymal sperm sample or ejaculate would reflect the fertility of the sample. This antibody can be used as a contraceptive to inactivate sperm, screen for toxicity, select animals for artificial insemination, and select men for assisted reproductive technologies. The protein itself can be inactivated by gene knockout, which is another approach to contraception, or the protein can be added to sperm from infertile men to make fertility techniques more feasible.

The invention discloses a plant male fertility-related protein, its coding gene and application. The protein provided by the present invention is the protein of the following (a) or (b): (a) a protein composed of the amino acid sequence shown in Sequence 1 in the sequence listing; (b) the amino acid sequence of Sequence 1 is processed by one or more A protein derived from SEQ ID NO: 1 that has substitution and / or deletion and / or addition of amino acid residues and is associated with plant male fertility. By inhibiting the expression of the gene encoding the protein in the target plant, male sterile transgenic plants can be obtained. The coding gene of the protein can be used to breed various male sterile transgenic plants, and has great significance and value to agriculture.

The invention discloses a high-efficiency seed labeling method which can be used for propagation of a plant male sterile line, and brings convenience for hybrid seed production. Furthermore, the invention provides a new method for high-efficiency propagation of the plant male sterile line by utilizing nucleotide sequences that controls plant male fertility and special grain shapes, e.g. size, length, width, thicknesst, etc.; and a transgene technology. According to the invention, the wild-type nucleotide sequence for controlling the male fertility and the nucleotide sequence for controlling the grain shapes are linked and transferred into conventional corns, and then a back cross into a homozygous recessive male sterile line is carried out; and the obtained transgene plant is hybridized with the homozygous recessive male sterile line to simultaneously obtain a lot of seeds of the sterile line and the maintainer line. Due to the effect of the nucleotide sequence for controlling the grain shapes, the seeds can be distinguished into the sterile line and the maintainer line through the grain shapes, wherein the seeds with normal shapes are the sterile line (without transgene sequences), and the seed with abnormal shapes (e.g. small grains) are the maintainer line.

The invention discloses a cucumber male sterility gene related SNP (Single Nucleotide Polymorphism) marking method and application. The marking method comprises the steps of taking a YL-5 male sterile line as a female parent to hybridize with a distant related variety D37-1, so as to obtain a hybrid F1, performing selfing by the hybrid F1 to obtain an F2 population, and two phenotypes of male sterility and male fertility exist in an F2 segregation population, then utilizing a method of combination of re-sequencing and BSA, screening an SNP locus unrelated with the male sterility, and developing a male sterility gene related molecular marker. The marking method disclosed by the invention has the advantages of short period, low cost and high efficiency. Meanwhile, an important development approach is provided for molecular markers for molecular breeding, phyletic evolution and germplasm resource identification.