The invention discloses a pain-relieving, blood-invigorating and tendon-relaxing plaster and a preparation method thereof. The pain-relieving, blood-invigorating and tendon-relaxing plaster comprises the following components in percentage by weight: 0.1-30% of rhizoma zingiberis, 0.1-20% of rhizoma kaempferiae, 0.1-20% of radix angelicae, 0.1-15% of rhizoma nardostachyos, 0.1-15% of rheum officinale, 0.1-10% of divaricate saposhnikovia roots, 0.1-10% of radix clematidis, 0.1-10% of lumbricus, 0.1-10% of resina draconis, 0.1-10% of coptis chinensis, 0.1-10% of garden balsam stems, 0.1-10% of lycopodium clavatum, 0.1-10% of caulis Spatholobi, 0.1-10% of myrrh, 0.1-10% of frankincense, 0.1-10% of borneol, 0.1-10% of peach kernels, 0.1-10% of angelica sinensis, 0.1-10% of syzygium aromaticum, 0.1-10% of pepper, 0.1-10% of cortex periplocae, 0.1-10% of Schizonepeta, 0.1-10% of cassia twig, 0.1-10% of magnolia flowers, 0.1-10% of notopterygium roots, 0.1-10% of ligusticum wallichii, 0.1-10% of radix angelicae pubescentis, 0.1-10% of moutan barks, 0.1-10% of pepper, 0.1-10% of rhizoma Atractylodis, 0.1-10% of menthol, 0.1-15% of methyl salicylate, 0.1-10% of ground beeltles, 0.1-15% of raw pinellia ternate, 0.1-10% of safflower carthamus and 0.1-10% of camphor. The pain-relieving, blood-invigorating and tendon-relaxing plaster disclosed by the invention has good anti-inflammatory and analgesic effects and an effect of promoting blood circulation to remove blood stasis, and not only can thoroughly relieve osteoproliferation, arthralgia and myalgia and arthralgia, but also has good curative effect on tuberculosis of spermary and epididymis, particularly on muscular paralysis.