The invention provides a culture medium for culturing akkermansia muciniphila and a use method thereof, and belongs to the technical field of microorganism culture mediums. Components of the culture medium include soy peptone, threonine, glucose, N-acetylglucosamine, sodium chloride and disodium hydrogen phosphate. By adopting the culture medium, the akkermansia muciniphila quantity required by functional studying of the akkermansia muciniphila can be reached through culture, the components of the culture medium have safe sources, and potential biological hazards do not exist.