The invention provides a natural organic-inorganic combined antifreeze preparation and a production method thereof. According to the invention, phosphorus-and-potassium-containing substances, seaweedextract, fish protein, chitosan, proline, multivitamins, boron-containing substances and zinc-containing substances are used as raw materials; the phosphorus-and-potassium-containing substances, the seaweed extract, the fish protein, the chitosan and the proline can improve the cold resistance of crops; the multivitamins improve the stress resistance of crops by protecting cell membranes from damage, improving photosynthesis efficiency and reducing respiratory capacity loss; the boron-containing substances and the zinc-containing substances are used for supplementing trace elements to crops; and the combination, scientific compatibility, complementation and coordination of recombinant organic raw materials and inorganic raw materials of the raw materials can be guaranteed at low temperature, and the biological activity of crops and the resistance of the crops to freezing injury can be organically combined, so the adaptability of the crops to a low-temperature stress environment is enhanced, the growth and development of the crops in the low-temperature environment are promoted, and production cost is low.