Application of akkermansia muciniphila or prevotella copri to preparation of drugs for enhancing anti-tumor immune function

A technology of anti-tumor immunity and myxobacteria, applied in the field of biomedicine, can solve the problem of no T cell depletion molecules and achieve the effect of inhibiting growth

Active Publication Date: 2020-01-03
REVAISSANT SHENZHEN BIOSCIENCES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, there is currently no research on the use of intestinal bacteria including Akkermansia and / or Prevotella to inhibit the expression of T cell exhaustion molecules, enhance the infiltration of CD8+ T cells in the tumor microenvironment, and enhance the anti-tumor function of T cells in the body. Reports to achieve better cancer prevention and / or treatment

Method used

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  • Application of akkermansia muciniphila or prevotella copri to preparation of drugs for enhancing anti-tumor immune function
  • Application of akkermansia muciniphila or prevotella copri to preparation of drugs for enhancing anti-tumor immune function
  • Application of akkermansia muciniphila or prevotella copri to preparation of drugs for enhancing anti-tumor immune function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 Ackerman's myxobacteria culture

[0051] training method

[0052] Step 1: Take a freeze-dried strain of Akkermansia muciniphila (purchased from the ATCC official website), add 200 μL of TSB medium, dissolve, draw 200 μl of blood on the petri dish, and then pump air from the gas control system of the anaerobic tank , cultivated under anaerobic conditions at 37°C for 48 hours in a biochemical incubator;

[0053] Step 2: Pick a single clone colony in 10mL TSB medium, and culture it under anaerobic conditions at 37°C for 48h;

[0054] Step 3: Take 500mL TSB medium, inoculate strains at 1% (v / v), and culture under anaerobic conditions at 37°C for 48 hours;

[0055] Step 4: Collect the bacteria solution and centrifuge at 6000rpm for 10min. After washing twice with normal saline, the bacteria sludge was reconstituted for use and counted viable bacteria.

Embodiment 2

[0056] Embodiment 2 Prevotella culture

[0057] training method

[0058] Step 1: Take a freeze-dried Prevotella copri strain (purchased from the ATCC official website), add 200 μL of PYG medium, dissolve, absorb 200 μl of blood on the plate, and biochemically after the gas control system of the anaerobic tank is pumped Anaerobic cultivation at 37°C for 48 hours in an incubator;

[0059] Step 2: Pick a single clonal colony in 10mL PYG medium and culture it under anaerobic conditions at 37°C for 48h;

[0060] Step 3: Take 500mL of PYG medium, inoculate strains at 1% (v / v), and culture under anaerobic conditions at 37°C for 48 hours;

[0061] Step 4: Centrifuge the harvested solution at 6000rpm for 10min. After washing twice with normal saline, the bacteria sludge was reconstituted for use and counted viable bacteria.

Embodiment 3

[0062] Example 3 Akkermansia Myxobacteria Enhance T Cell Anti-tumor Immunity and Akkermansia Myxobacteria Treats Tumor Experiments

[0063] figure 1 To detect that Akkermansia or inactivated Akkermansia enhance T cell anti-tumor immune function and promote the accumulation of CD8+ T cells in the tumor microenvironment and Akkermansia or inactivated Akkermans prevents and treats tumors Schematic diagram of the experimental procedure.

[0064] 1. Training method

[0065] Akerman's myxobacteria culture method is the same as embodiment 1.

[0066] 2. Sample preparation

[0067] 1) Preparation of Ackermannian myxobacteria live cells

[0068]Step 1: Take a freeze-dried strain of Akkermansia muciniphila (purchased from the ATCC official website), add 200 μL TSB medium, dissolve, draw 200 μl of blood on the petri dish, and pump air from the gas control system of the anaerobic tank Cultivate for 48 hours under anaerobic conditions at 37°C in a post-biochemical incubator;

[0069]...

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Abstract

The invention provides application of akkermansia muciniphila or prevotella copri to preparation of drugs for enhancing the anti-tumor immune function of CD4+T cells and / or CD8+T cells and / or for enhancing the anti-tumor immune function of CD8+T cells in the tumor microenvironment. The akkermansia muciniphila or the prevotella copri can significantly inhibit PD-1 expressed by systematic CD4+T cells and / or CD8+T cells, can also promote infiltrating and / or accumulating of the CD8+T cells in the tumor microenvironment, inhibits expression of PD-1 molecules, and significantly inhibits tumor growth.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to enhancing T cells (including: CD4+T cells, CD8+T cells, etc.) ) anti-tumor immune function and its application in drugs for preventing and / or treating tumors. Background technique [0002] Malignant tumors are the main cause of death in humans. The "Global Cancer Report 2014" issued by the World Health Organization (WHO) predicts that global cancer cases will show a rapid growth trend, from 14 million in 2012 to 19 million in 2025, and will reach 24 million in 2035 . In 2015, 8.8 million people died of cancer worldwide, accounting for one-sixth of global deaths. For example, lung cancer is one of the most common tumors in China, and surgery and chemotherapy are currently the main treatments. However, surgery and chemotherapy have a poor prognosis. In particular, while chemotherapy drugs kill cancer cells, they also destroy the immune function of the human body. [0003] O...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P35/00A61P37/04A23L33/135
CPCA23V2002/00A61K35/74A23L33/135A61P35/00A61P37/04A23V2200/308
Inventor 曾谷城
Owner REVAISSANT SHENZHEN BIOSCIENCES CO LTD
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