65 results about "Pseudomonas aeruginosa exotoxin A" patented technology

Methods and compositions relating to recombinant anti-CD22 antibodies with high binding affinity, and immunoconjugates comprising the anti-CD22 antibody linked to a therapeutic agent such as a Pseudomonas exotoxin or a detectable label. The invention provides diagnostic methods, and means to inhibit the growth of malignant B cells.

The invention provides a chimeric CNS targeting polypeptide having a BBB-receptor binding domain and a payload polypeptide domain. The chimeric CNS targeting polypeptide can have a BBB-receptor binding domain consisting of a receptor binding domain from ApoB, ApoE, aprotinin, lipoprotein lipase, PAI-1, pseudomonas exotoxin A, transferrin, α2-macroglobulin, insulin-like growth factor, insulin, or a functional fragment thereof. Nucleic acids encoding a chimeric CNS targeting polypeptide are also provided. Further provided is a method of delivering a polypeptide to the CNS of an individual. The method consists of administering to the individual an effective amount of a chimeric CNS targeting polypeptide, said chimeric CNS targeting polypeptide comprising a BBB-receptor binding domain and a payload polypeptide domain. The method also can deliver a polypeptide to the lysosomes of CNS cells.

The invention provides mutated, cytotoxic forms of Pseudomonas exotoxin A (PE) comprising a furin cleavage sequence conjugated or fused directly to residues 395-613 of PE or variants of that sequence. These minimal forms of PE are smaller than previous cytotoxic forms of PE, reduce non-specific toxicity, and reduce immunogenicity due to domain II or domain Ib of PE. The invention further provides nucleic acids encoding said PEs, chimeric molecules containing them, and methods of use thereof.

The invention provides chimeric proteins comprising a non-toxic Pseudomonas exotoxin A sequence and a Type IV pilin loop sequence, wherein the Type IV loop sequence is inserted within the non-toxic Pseudomonas exotoxin A. The invention also provides polynucleotides encoding the chimeric proteins, and compositions comprising the polynucleotides or the chimeric proteins. The invention also provides methods for using the chimeric proteins, polynucleotides and compositions of the invention.

The present invention relates to polymer conjugates of SS1P, and methods of making using the same.

The present invention provides PE CD4+T-cell epitopes, as well as novel variants that exhibit reduced immunogenic responses, as compared to the parental PE. The present invention further provides DNA molecules that encode novel PE variants, host cells comprising DNA encoding novel PE variants, as well as methods for making PEs less immunogenic. In addition, the present invention provides various compositions that comprise these PE variants that are less immunogenic than wild-type PEs.

The invention is directed to an immunotoxin directed at fetal AchR, wherein the immunotoxin may comprises a human Fab fragment based on a human autoantibody or human combinatorial cDNA library and may be a pseudomonas exotoxin A-based single chain Fv IT (35-scFV-ETA). The invention is further directed to method of treating a patient with soft tissue tumour comprising the step of exposing the patient to the immunotoxin of the invention.

The invention discloses target specific double mutant fusion protein, which is established by human gonadotropin releasing hormone mutant (mGnRH) and recombinant pseudomonas aeruginosa exotoxin A mutant (PE38m4a) fusion protein. The mGnRH-PE38m4a protein provided by the invention has obvious specific binding activity and cytotoxicity to the tumor cell lines which comprise colon cancer HT-29 cells, oophoroma OVCAR3 cells, cervical adenocarcinoma HeLa cells or liver cancer HepG-2 cells, and the like; the normal cells basically have no lethal function; the activity of the fusion protein is enhanced by approximate nine to ten times compared with the activity of natural PEA; the cost is low, the method is simple, therefore the target specific double mutant fusion protein is more suitable for industrialized production.

The invention provides a fusion protein, a fusion protein-containing vaccine composition for mycoplasmal pneumonia of swine (MPS) and an application of the vaccine composition. The fusion protein comprises pseudomonas exotoxin A structural domains I and II, an antigenic protein for MPS and a carboxyl terminal part, wherein the antigenic protein for MPS comprises any one of an NrdF protein, a P102 protein, a P97R1 protein, a P97R2 protein, a P97R1-NrdF protein and a P97R2-NrdF protein. The invention also discloses the vaccine composition containing immune dosage of fusion protein and the application of the vaccine composition to preparation of drugs for preventing and/or treating MPS. The vaccine composition has the advantages that recombinant expression can be carried out on the components of the vaccine composition in quantity by means of genetic engineering, thus not only consuming short time but also facilitating large-scale production; the vaccine composition can generate humoral immunity and cellular immunity and can gain better immune effects.

The invention provides a fusion protein and a preparation method and application thereof, an expression system and a vaccine, and relates to the technical field of biology. The fusion protein providedherein includes Pseudomonas exotoxin A domain section, porcine circovirus type 2 Cap protein dominant epitope section, porcine circovirus type 3 Cap protein dominant epitope section and carboxy terminus. The epitopes of PCV2 (porcine circovirus type 2) and PCV3 Cap proteins are screened out; the epitopes have good antigenicity and are easy to highly express; the epitopes are fused to partial functional fragments of PEA (Pseudomonas exotoxin A); carboxy terminal sequence and enhanced immunogenicity are imparted to C end of the fusion protein; therefore, the fusion protein is more soluble. Thefusion protein herein has the advantages of targeted positioning, good antigenicity, and high expression quantity. The fusion protein is advantageous in good safety, stable titer, zero toxic and sideeffects and cellular immune response activation when applied as an antigen to prepare vaccines.

The invention relates to a targeted tumor-resistant recombinant protein and a preparation method thereof, and belongs to a targeted tumor-resistant recombinant protein medicament. A truncated cell factor hIL6 is connected to a derivative PE38KDEL of pseudomonas aeruginosa exotoxin A through a joint, and an amino acid mutation derivative of hIL6 serving as a guide vector comprises 1-28 amino acidsof truncated hIL6 amino ends. The invention further relates to application of the recombinant targeted protein to tumor resistance. The targeted tumor-resistant recombinant protein hIL6 (T23)-PE38KDEL is constructed by gene modification and codon optimization, the vacant site resistance between two ligands is reduced, and the activity is improved; and efficient soluble expression is realized in escherichia coli, the bottleneck of low expression of natural genes is broken, and large-scale preparation of the recombinant toxin becomes possible.

The invention provides a fusion protein, a highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS) vaccine composition containing the fusion protein and an application of the vaccine composition. The fusion protein comprises pseudomonas exotoxin A structural domains I and II, an HP-PRRS antigenic protein and a carboxyl terminal part, wherein the HP-PRRS antigenic protein comprises an Nsp9 protein and an Nsp10 protein. The invention also discloses the vaccine composition containing immune dosage of fusion protein and the application of the vaccine composition to preparation of drugs for preventing and/or treating HP-PRRSs. The vaccine composition has the advantages that recombinant expression can be carried out on the components of the vaccine composition in quantity by means of genetic engineering, thus not only consuming short time but also facilitating large-scale production.

The invention discloses a recombinant S1 protein of a novel mutant strain of a porcine epidemic diarrhea virus and a subunit vaccine of the recombinant S1 protein. The protein is obtained by the steps of cloning a fusion gene fragment PE(delta III)-S1(m)-KDEL3 containing a KDEL3 gene sequence, a gene sequence of III-region-deleted pseudomonas aeruginosa exotoxin A and an S1(m) gene sequence to a baculovirus vector to obtain a recombinant vector; carrying out recombinant vector site specificity transposition on a seroconversion DH10Bac competent cell of the recombinant vector to obtain a recombinant bacmid; transfecting the recombinant bacmid to an Sf9 cell under the mediation of a liposome to obtain a recombinant Sf9 cell; expressing by using the recombinant Sf9 cell. According to the invention, a PE(delta III)-S1(m)-KDEL3 fusion protein is firstly expressed by using a baculovirus expression system on the basis of optimizing the predilection of a codon of mammalian with S1 genes, so that the recombinant baculovirus for efficiently and accurately expressing the fusion protein is obtained. Proved by IFA and Western blot, the recombinant baculovirus can be used for accurately expressing the fusion protein, and the fusion protein has favorable reactionogenicity.

The invention relates to a gastric cancer targeted recombinant toxin and a preparation method thereof, and belongs to a targeted recombinant toxin and a preparation method thereof. The gastric cancer targeted recombinant toxin is formed by connecting human gastrin G17 with recombinant toxin PE38KDEL derived from pseudomonas exotoxin A, and particularly the recombinant toxin is prepared by using G17 expressed by a prokaryotic expression system and translated reversely as a guiding unit through gene engineering expression; and a unique fermentation condition for producing a soluble protein in a large scale is established, and the recombinant toxin targeting gastric cancer and having high selective killing activity is provided.

Pseudomonas exotoxin A or “PE” is a 66 kD, highly potent, cytotoxic protein secreted by the bacterium Pseudomonas aeruginosa. Various forms of PE have been coupled to other proteins, such as antibodies, to generate therapeutically useful cytotoxin conjugates that selectively target cells of a desired phenotype (such as tumor cells). In the present invention, peptides spanning the sequence of an approximately 38 kD form of Pseudomonas exotoxin A protein were analyzed for the presence of immunogenic CD4+ T cell epitopes. Six immunogenic T cell epitopes were identified. Residues were identified within each epitope for introduction of targeted amino acid substitutions to reduce or prevent immunogenic T-cell responses in PE molecules which may be administered to a heterologous host.