67 results about "Ricin" patented technology

The invention relates to methods and kits for detecting and / or monitoring biological molecules in a test sample. For example, the invention relates to methods and kits for detecting and / or monitoring HIV p24 antigen in human body fluid, biological toxins such as ricin or botulism in an environmental or biological sample, and prion protein from human, deer or bovine, such as PrPSC, in a biological sample. The antigen detection signal is boosted by amplification of a polynucleotide linked to a detector molecule using methods for nucleic acid amplification technology.

The disclosure provides compositions relating to thermostable vaccines and methods of preparing same. Specifically, the disclosure provides for methods of preparing thermostable vaccines based on a recombinant ricin neurotoxin protein and uses of co-adjuvants to develop a composition capable of eliciting an immune response in a subject.

Ricin A-chain is an N-glycosidase that attacks ribosomal RNA at a highly conserved adenine residue. Crystallographic studies show that not only adenine and formycin, but also pterin-based rings can bind in the ricin active site. For a better understanding of the recognition mode between ricin, and adenine-like rings, the interaction energies and geometries were calculated for a number of complexes. Shiga toxin, a compound essentially identical to the protein originally isolated from Shigella dysenteriae, has an active protein chain that is a homologue of the ricin active chain, and catalyzes the same depurination reaction. The present invention is drawn to identifying inhibitors of ricin and Shiga toxin, using methods molecular mechanics and ab initio methods and using the identified inhibitors as antidotes to ricin or Shiga toxin, or to facilitate immunotoxin treatment by controlling non-specific cytotoxicity.

Compositions and methods for treatment of focal muscle spasms. Immunotoxin conjugates comprise a toxin conjugated to an antibody reactive to a muscle specific antigen.

A method of preparing molecules of interest for delivery to eukaryotic cells is shown, wherein a ricin B chain subunit not having a ribosome inactivating subunit and retaining lectin activity is modified by modifying the first cysteine residue to be absent or substituted with an amino acid other than cysteine, or removing a protease sensitive site at the N-terminal of the subunit, or adding an endoplasmic reticulum retrieval signal, and operatively associating the subunit with a molecule of interest. Methods of operatively associating the subunit and molecule of interest include chemical conjugation at primary amines, conjugation with N-glycans of the subunit, a disulfide bond, and assembly of immunoglobulin domains. The invention provides for operatively associating multiple molecules of interest with a ricin B chain subunit, and delivery into targeted cells, cell components, and combination of cells.

The present invention relates to anti-ricin antibodies and uses thereof. More specifically, the invention relates to anti-ricin antibodies and fragments thereof as well as their use in therapy or prophylaxis.

The invention relates to a protein suspension chip of ricin and methods for preparing, quantifying and detecting the same. The detecting method comprises the following steps: adding a working solution and a sample to be tested into a hole; adding a detection antibody; adding SA-PE; adding a detection buffer solution and mixing the mixture evenly; and reading numerical values, analyzing data and judging the detection result. The quantifying method comprises the following steps: adding a positive test sample or a standard product for dilution through gradient multiple proportions, detecting a series of diluted samples in a suspension chip system, and reading a corresponding fluorescence value (MFI); making a dosage-reaction curve; and fitting the dosage-reaction curve and an equation. The suspension chip comprises a coded microsphere, a ricin capturing antibody coated on the microsphere, a biotin-marked ricin detection antibody, a streptavidin-phycoerythrin and the buffer solution. The methods have the advantages of good detectability, high sensitivity, strong specificity and wide dynamic range.

The invention provides a haplometrotic ricin asexual propagation method belonging to the crop heterosis utilization field. The invention is characterized in that: A. transplant and preserve the whole plant of single female ricin material which can not be sexually reproduced into the sunlight greenhouse before frost; B. cut hard branches with three leaves when the temperature is up to 20 DEG C in the next spring, dip the lower part of the ricin cutting into 2000 times solution of 20% a-NAA powder in 2 hours; C. root the hard branch of ricin in nursery bed containing fine sand or fine soil, the depth is 10-15cm; D. cultivate the cutting at 20-28 DEG C, under 70-80% humidity, with the moist substrate and scattered light; E. train the branches 5-10 days before being planted into field, increase the light and ventilation time. The invention not only has the merits of retaining precious ricin single female breeding material, but also breeding lots of single female ricin material many times using branches of single plant.

The invention discloses a high-sensitivity novel bio-barcode detection method for ricin, which is used for the detection of ricin protein. According to the novel bio-barcode detection method, a marked bar code DNA strand is improved to be a single strand on the basis of the conventional bio-barcode detection method; the marked bar code DNA strand is prolonged until the marked bar code DNA strand can be detected by a fluorescent quantitative PCR / TaqMan fluorescent probe; and a bar code DNA strand in a sandwich reaction compound does not need to be dissociated and is directly used for performing qualitative detection on the bar code RNA strand in the sandwich reaction compound by a TaqMan fluorescent probe-based real-time fluorescent quantitative PCR method or the conventional PCR method. The method has great practical significance for the high sensitivity detection of the ricin protein and has wide application prospect.

A vegetative rodenticide is prepared from the vegetative toxin chosen from ricin, ricinine, and castor allergen, and attractant. Its advantages are high attraction and killing power, high palatability, and no environmental pollution.

A method of detecting N-glycosylase activity in a sample involves incubating an oligodeoxyribonucleotide substrate containing a deoxyadenosine or deoxyuridine residue with the sample to be tested such that the N-glycosylase, if present, hydrolyzes the deoxyadenosine or deoxyuridine residue to result in an N-glycosylase product having an abasic site. A primer is annealed to the N-glycosylase product, and the primer is extended with a DNA polymerase, such as Taq DNA polymerase, that pauses at abasic sites. The resulting extension products are melted from the N-glycosylase product, allowed to form hairpins due to self-complementarity, and further extended in the presence of labeled precursors to result in labeled products. Extension products synthesized from undigested substrate as template do not result in labeled products. Thus, detection of labeled products results in detection of N-glycosylase activity. Oligodeoxyribonucleotide substrates, primer, and positive controls and a kit for N-glycosylase assay are also disclosed.

The invention relates to a novel application of ricin B chain protein in the aspect of immunoloregulation, which belongs to the scope of medicine field. An application of ricin B chain protein in the preparation of immunoregulators, and an application of ricin B chain protein in the preparation of agents for tumor adjuvant therapy are provided. Ricin B chain protein can activate mouse peritoneal macrophages, promotes mouse spleen T and B lymphocyte proliferation, improve NK cell activity, has the effect of immunoloregulation, can be used as an immunoregulator, and can be used in tumor adjuvant therapy.

The invention discloses an immunity chromatographic test paper for detecting ricin and a preparation method thereof. The immunity chromatographic test paper for detecting the ricin provided by the invention comprises an absorbent paper washer, a fiber membrane which is closely connected to the absorbent paper washer and coated with anti-ricin A-chain protein antibodies, a gold conjugate pad which is closely connected on the fiber membrane, consists of anti-ricin B-chain protein antibodies and is immunized from gold colloid probes, and a sample washer which is closely connected to the gold conjugate pad. Moreover, a backboard is also closely connected under the absorbent paper washer for convenience in use. By adoption of the immunity chromatographic test paper for detecting the ricin, laypeople can operate according to specifications; treatment processes of specimen is simple, and results can be observed within 5 minutes; at least 20 nanogram per milliliter ricin can be detected, and detection sensibility is not affected by specimen sources. The ricin immunity detection test paper has the advantages of simplicity, convenience, sensitivity, specificity and quickness and is suitable for clinical and field use.

The present invention provides a protein having an A chain of a ricin-like toxin, a B chain of a ricin-like toxin and a heterologous linker amino acid sequence, linking the A and B chains. The linker sequence contains a cleavage recognition site for a disease specific protease such as a cancer, fungal, viral or parasitic protease. The invention also relates to a nucleic acid molecule encoding the protein and to expression vectors incorporating the nucleic acid molecule. Also provided is a method of inhibiting or destroying mammalian cancer cells, cells infected with a virus, a fungus, or parasite; or parasites utilizing the nucleic acid molecules and proteins of the invention and pharmaceutical compositions for treating human cancer, viral infection, fungal infection, or parasitic infection.

The invention establishes a fast detection method of ricin by utilizing immuno-chromatographic technology of colloidal gold labeling and double antibody sandwich. The established colloidal gold immunochromatographic method for detecting ricin can detect the ricin in a sample fast, sensitively, specifically and accurately, can realize quantitation and is applicable to field fast detection.

Plasmids encoding anti-HIV and anti-anthrax therapeutic agents are disclosed. Plasmid pWKK-500 encodes a fusion protein containing DP178 as a targeting moiety, the ricin A chain, an HIV protease cleavable linker, and a truncated ricin B chain. N-terminal extensions of the fusion protein include the maltose binding protein and a Factor Xa protease site. C-terminal extensions include a hydrophobic linker, an L domain motif peptide, a KDEL ER retention signal, another Factor Xa protease site, an out-of-frame buforin II coding sequence, the lacZα peptide, and a polyhistidine tag. More than twenty derivatives of plasmid pWKK-500 are described. Plasmids pWKK-700 and pWKK-800 are similar to pWKK-500 wherein the DP178-encoding sequence is substituted by RANTES- and SDF-1-encoding sequences, respectively. Plasmid pWKK-900 is similar to pWKK-500 wherein the HIV protease cleavable linker is substituted by a lethal factor (LF) peptide-cleavable linker.

Plasmids encoding anti-HIV and anti-anthrax therapeutic agents are disclosed. Plasmid pWKK-500 encodes a fusion protein containing DP178 as a targeting moiety, the ricin A chain, an HIV protease cleavable linker, and a truncated ricin B chain. N-terminal extensions of the fusion protein include the maltose binding protein and a Factor Xa protease site. C-terminal extensions include a hydrophobic linker, an L domain motif peptide, a KDEL ER retention signal, another Factor Xa protease site, an out-of-frame buforin II coding sequence, the lacZa peptide, and a polyhistidine tag. More than twenty derivatives of plasmid pWKK-500 are described. Plasmids pWKK-700 and pWKK-800 are similar to pWKK-500 wherein the DP178-encoding sequence is substituted by RANTES- and SDF-1-encoding sequences, respectively. Plasmid pWKK-900 is similar to pWKK-500 wherein the HIV protease cleavable linker is substituted by a lethal factor (LF) peptide-cleavable linker. Plasmid pWKK-21 is similar to pWKK-500 wherein the highly truncated ricin B chain is substituted by a one-domain ricin B chain. Oligonucleotide cassettes encoding an HIV protease-cleavable peptide linker and a method of making modified plasmids encoding modified fusion proteins are also described.

The invention relates to a mucosal immunoadjuvant inducing Th1 immune response. A bioinformatics technology is applied in the invention, the spatial conformation of a ricin B-chain monoclonal anti-3E1 and Ricin-B compound which has obtained a Chinese invention patent is determined through the action of an antibody-antigen compound, and a molecular stimulation technology is utilized to reasonably determine four Ricin B identifying active regions of 3E1 and derive four nucleotide sequences. Four GFP-nRTB fusion proteins are obtained through a molecular cloning technology. Results of experiments that the four proteins are used to immunize BALB / C mice through a sublingual way three times show that a P1-GFP protein in the four proteins excites Th1 immune response, and a high-level IgG2a antibody is generated. The capability and the possible molecular mechanism of the targeting GFP mucosal immune response of the P1-GFP protein are discussed. The mucosal immunoadjuvant inducing Th1 immune response, which is screened through the above scheme, can be used for preparing vaccines, immunomodifiers or treatment medicines.

The invention provides a two-line hybrid ricin seed production method, which belongs to the heterosis utilization field. It includes: ricin female line selecting, breeding, two-line hybrid seed cross-pairing and two-line hybrid seed producing. The invention overcomes the problem of artificial extracting amphoteric plants during blooming period in the process of amphoteric female line (that is about 50% of the plants are female, 50% are amphoteric) hybrid seed production, simplifies ricin hybrid producing procedures and realizes two-line single cross seeds production. At the same time the invention has the merits of simple seed producing method, high seed purity, big F1 heterosis, high yield production, good uniformity, good quality and so on. The method is suitable for wide spread.

A method of detecting N-glycosylase activity in a sample involves incubating an oligodeoxyribonucleotide substrate containing a deoxyadenosine or deoxyuridine residue with the sample to be tested such that the N-glycosylase, if present, hydrolyzes the deoxyadenosine or deoxyuridine residue to result in an N-glycosylase product having an abasic site. A primer is annealed to the N-glycosylase product, and the primer is extended with a DNA polymerase, such as Taq DNA polymerase, that pauses at abasic sites. The resulting extension products are melted from the N-glycosylase product, allowed to form hairpins due to self-complementarity, and further extended in the presence of labeled precursors to result in labeled products. Extension products synthesized from undigested substrate as template do not result in labeled products. Thus, detection of labeled products results in detection of N-glycosylase activity. Oligodeoxyribonucleotide substrates, primer, and positive controls and a kit for N-glycosylase assay are also disclosed.

The present invention provides a protein having an A chain of a ricin-like toxin, a B chain of a ricin-like toxin and a heterologous linker amino acid sequence, linking the A and B chains. The linker sequence contains a cleavage recognition site for a disease specific protease such as a cancer, inflammatory, fungal, viral or parasitic protease. The invention also relates to a nucleic acid molecule encoding the protein and to expression vectors incorporating the nucleic acid molecule. In addition, the invention relates to a method for producing the recombinant protein in yeast, to nucleic acid molecules for use in yeast and to yeast transformed with such nucleic acid molecules. Also provided is a method of inhibiting or destroying mammalian cancer cells, inflammatory cells, cells infected with a virus, a fugus, or parasite, or parasites utilizing the nucleic acid molecules and proteins of the invention and pharmaceutical compositions for treating human cancer, inflammatory disease, viral infection, fugal infection, or parasitic infection.

The invention provides a plant protein foam fire extinguishing agent based on ricin hydrolysate. The plant protein foam fire extinguishing agent is characterized in that the ricin hydrolysate is used as a raw material, dilution is achieved after a hydrocarbon surface active agent and an additive are added, and a finished product can be obtained. The plant protein foam fire extinguishing agent has the beneficial effects of being high in foam expansion, good in foam stability, long in storage time, free of sediment, small in viscosity, capable of enabling protein not prone to going bad, easy and convenient to prepare and the like.

The invention relates to a targeted antitumor complex based on ricin, a preparing method thereof and applications of the complex. The complex is prepared by compositing carbon quantum dots and a ricinA chain to form a carbon quantum dot-ricin A chain complex, then encapsulating the complex with the lipidosome, and modifying the lipidosome with a nucleolin nucleic acid aptamer. The prepared complex can enter cells more easily, acts on ribosome through a Golgi apparatus path, has higher cytotoxicity, can effectively protect the A chain from being enzymatically decomposed by lysosome, utilizes specificity of the nucleolin nucleic acid aptamer, can be targeted on a tumor cell, reduces toxic and side effects on normal cells, and is of great importance for targeted treatment on tumor cells.

A medicine for making mouse sterile is prepared from castor seed through extracting castor oil and ricin, preparing their micro capsules, and proportionally mixing them with antiseptic and attractant.

The present invention relates to anti-ricin antibodies and uses thereof. More specifically, the invention relates to anti-ricin antibodies and fragments thereof as well as their use in therapy or prophylaxis.

The invention discloses peptide of specific banded ricin, nucleic acid for encoding the same, and method for detecting and restraining the ricin. Preferably, the peptide of the invention has all showed amino acid sequences of SEQ ID NO: 1 to 12.

The present invention provides a protein having chain of a ricin-like toxin, a B chain of a ricin-like toxin and a novel heterologous linker amino acid sequence, linking the A and B chains. The linker sequence contains a cleavage recognition site for a specific protease such as those found in inflammatory cells and cancer cells. The invention also relates to a nucleic acid molecule encoding the protein and to expression vectors incorporating the nucleic acid molecule. Also provided is a method of inhibiting or destroying cells having a specific protease, such as cancer cells or inflammatory cells utilizing the nucleic acid molecules and proteins of the invention and pharmaceutical compositions for treating human inflammation and cancer.