The invention belongs to the field of measurement of
plant hormones, and provides a measuring method of immune PCR of
abscisic acid. The method comprises the steps of usingglutaraldehyde to pretreat the inner surface of a PCR tube / plate, using a single anti-abscisic-acid
antibody to cover the surface of the treated PCR tube / plate to synthesize a probe mixture, adding the probe mixture into the treated PCR tube / plate, then adding an
abscisic acid sample to be measured into the PCR tube / plate to be reacted, conducting amplification in real-time PCR equipment, and according to the amplification result, determining the content ofabscisic acid. A comparison experiment shows that by utilizing
glutaraldehyde to pretreat the inner surface of the PCR tube / plate, the
antibody covering capability can be improved by 20-30%; the three substances of a
biotinylation ABA polyclonal
antibody,
biotinylation DNA and
avidin are further utilized to synthesize the probe mixture, the
repeatability of ABA measurement is improved, the detection
lower limit of the immune
PCR method can reach 10 ng / L, close to the detection result of liquidchromatography
mass, and the problem that
organism in-vitro
plant hormones aredifficult to directly react with biomacromolecules is solved.