The invention relates to a microaerophilic separation culture method of
Campylobacter jejuni, which adopts the combination of a general
candle-cylinder method and a pyrogallic acid method to create a microaerophilic environment. A directive streak cultivation method is adopted against the separation culture of the
Campylobacter jejuni, a small amount of animal tissue detection samples are picked and directly inoculated on the selected culture medium by using connection annulus, the concentration of
oxygen in the air is reduced by the
candle-cylinder method with the combination of pyrogallic acid and
anhydrous sodium carbonate mixture, an alkaline environment is formed by using
anhydrous sodium carbonate so as to lead the pyrogallic acid to react with
oxygen for absorbing the
oxygen, therefore the
culture environment meets the microaerophilic conditions required by the
Campylobacter jejuni. Typical colonies obtained from separation culture are picked for PCR identification so as to further determine the
Campylobacter jejuni. The microaerophilic separation culture method of
Campylobacter jejuni has the advantages of low cost, simple operation, high detectable rate, good culture efficiency and the like, thus improving the separation culture efficiency of the
Campylobacter jejuni and resolving the difficulty for cultivating the Campylobacter jejuni under microaerophilic condition.